Correlation of structure and echogenicity of nanoscaled ultrasound contrast agents in vitro

Brüßler, Jana; Marxer, Elena Eva Julianne; Becker, Andreas; Schubert, Rolf; Schümmelfeder, J.; Nimsky, Christopher; Bakowsky, Udo

doi:10.1016/j.colsurfb.2014.02.029

Cited by 18 publications

(10 citation statements)

References 26 publications

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“…have studied in detail the effect of increasing concentration of PEG40S on the structure of lipid formulations. [ 26,27 ] The single chained PEG (40) stearate in phospholipid bilayer has a small portion of the hydrophobic part, therefore it tends to be at the interface of aqueous/lipid phases of liposomes. Thus, the air which gets entrapped during the agitation process remains intact inside the core of tightly packed vesicle, giving ultrasound contrast.…”

Section: Resultsmentioning

confidence: 99%

Development and Characterization of Ultrasound Activated Lipopolyplexes for Enhanced Transfection by Low Frequency Ultrasound in In Vitro Tumor Model

Shah

Tariq

Engelhardt

et al. 2020

Macromolecular Bioscience

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This work focuses on the development of ultrasound contrast vesicles for ultrasound‐mediated enhanced transfection of nucleic acids in the cancer cells and projects its application as a tool for diagnostic imaging. The ultrasound contrast vesicles are stable, anionic, nanoscaled vesicles with ultrasound contrast equivalent to the commercially available SonoVue. These anionic lipid vesicles establish electrostatic interaction with cationic polyplexes based on linear polyethylenimine (22kDa) forming lipopolyplexes with ultrasound contrast. The lipopolyplexes are characterized regarding shape, size, and zeta potential. When exposed to low frequency ultrasound, these carriers show elevated transfection efficiency and reduced cytotoxicity. The effect of post‐transfection ultrasound on cellular uptake of lipopolyplexes is also evaluated. An analogous transfection is also observed in the tumor mimicking multicellular 3D spheroid culture of ovarian cancer cells. The emergence of tumor imaging and enhanced gene delivery by medical ultrasound, a noninvasive imaging modality, is considered paving the way for efficient theranostic gene therapy.

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Section: Resultsmentioning

confidence: 99%

Development and Characterization of Ultrasound Activated Lipopolyplexes for Enhanced Transfection by Low Frequency Ultrasound in In Vitro Tumor Model

Shah

Tariq

Engelhardt

et al. 2020

Macromolecular Bioscience

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“…21 Because of its excellent biocompatibility, biodegradability, avirulence, non-immunogenicity, antibacterial activity, etc., chitosan has become noticed in the field of pharmacy and medicine. It has been widely used as drug carrier, gene, drugs and other bioactive molecules.…”

Section: Discussionmentioning

confidence: 99%

Experimental Study on PEG10 Antisense Gene Therapy for Hepatocellular Carcinoma Mediated by Nanoscale Ultrasound Contrast Agent

Mei¹,

Lü

et al. 2016

j comput theor nanosci

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Objective: To explore the application of nanoscale ultrasound contrast agent mediating PEG10 antisense gene transfection in the treatment of hepatocellular carcinoma. Methods: The optimal concentration and ultrasound parameters of nanoscale ultrasound contrast agent were screened via cell viability assay. PEG10 antisense oligodeoxynucleotide (ASODN) and fluorescein-labeled specific ligand galactose terminal glycol-poly-L-lysine (G-PLL) were combined with nanoscale ultrasound contrast agent. Then PEG10 ASODN was transfected into human HepG2 cells under ultrasonic irradiation. RT-PCR was used to detect the expression of PEG10. MTT assay was conducted to assess the proliferation inhibition of hepatocellular carcinoma cells. And the tumor inhibition rate in vivo was analyzed by establishing the nude mice transplantation tumor model. Results: Of all the conjugates, the optimal molar ratio of PEG10 ASODN to G-PLL was 1:2. The best parameters after screening showed that nanoscale ultrasound contrast agent of 15% microbubble concentration and ultrasound exposure at 1.5 MHz, 1.0 W/cm 2 , and 10% duty cycle for 60 s had no significant influence on the viability of HepG2 cells (P > 0 05). Compared with other groups, PEG10 ASODN+G-PLL+microbubble group had the weakest PEG10 expression, the most significant cell growth inhibition, and the highest tumor inhibition rate up to 38.5%. Conclusion: Under ultrasonic irradiation, G-PLL conjugating nanoscale microbubble ultrasound contrast agent and antisense gene can effectively enhance the inhibition of antisense gene on PEG10 expression, which has targetedly inhibitory effect on hepatoma cells. This is expected to provide an efficient means for the treatment of hepatocellular carcinoma.

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“…The samples were diluted 1:200 with PBS (pH 7.4). Samples were measured according to a previous protocol with slight modifications . The instrument performs 100 runs per sample.…”

Section: Methodsmentioning

confidence: 99%

Multilayer Bacteriostatic Coating for Surface Modified Titanium Implants

Vögeling

Duse

Seitz

et al. 2018

Physica Status Solidi (a)

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One of the serious problems occurring after the insertion of implants is the formation of biofilms. This leads to implant loss or sepsis. The multilayer coating developed in this study addresses the problems pertaining to bacterial growth and biofilm formation by two different approaches viz. preventing bacterial adhesion by nanostructured surface modification and by modification of the titanium surface by creating an ultra-thin poly(lactic-coglycolic acid) (PLGA)/norfloxacin (NFX) nanoparticle coating. In this study, NFX loaded PLGA nanoparticles are used to coat titanium surfaces using the layer by layer method alternating with chitosan. The advantages of biodegradable polymers like PLGA and chitosan to create an anti-adhesive and antibacterial coating are utilised. The polymers in this study show a high biocompatibility and are completely degraded within 10 weeks which makes them an excellent choice for use in dental implants and stents.

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Correlation of structure and echogenicity of nanoscaled ultrasound contrast agents in vitro

Cited by 18 publications

References 26 publications

Development and Characterization of Ultrasound Activated Lipopolyplexes for Enhanced Transfection by Low Frequency Ultrasound in In Vitro Tumor Model

Development and Characterization of Ultrasound Activated Lipopolyplexes for Enhanced Transfection by Low Frequency Ultrasound in In Vitro Tumor Model

Experimental Study on PEG10 Antisense Gene Therapy for Hepatocellular Carcinoma Mediated by Nanoscale Ultrasound Contrast Agent

Multilayer Bacteriostatic Coating for Surface Modified Titanium Implants

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