Correlation of stylar ribonuclease zymograms with incompatibility alleles in sweet cherry

Bošković, R.; Tobutt, K. R.

doi:10.1007/bf00023865

Cited by 171 publications

(167 citation statements)

References 8 publications

Supporting

Mentioning

153

Contrasting

Unclassified

Order By: Relevance

“…From a cross-pollination study, Matthews and Dow (1969) assigned cultivars to 13 incompatibility groups and a Group O, which includes cultivars that are SI but able to pollinate cultivars in all other groups (universal pollen donor). Bošković and Tobutt (1996) reported that RNase zymograms were associated with S-alleles. On the basis of zymograms of stylar proteins, S-alleles of some cultivars were assigned and new S-alleles were detected.…”

Section: Subgenus Cerasus (Cherries) 1) Identification Of S-rnase Allmentioning

confidence: 99%

Molecular Basis of Self-(in)compatibility and Current Status of S-genotyping in Rosaceous Fruit Trees

Yamane

Tao

2009

J. Japan. Soc. Hort. Sci.

View full text Add to dashboard Cite

Most rosaceous fruit tree species exhibit gametophytic self-incompatibility (GSI). During the last decade, much progress has been made in identifying and characterizing the S-locus genes that control the specificity of the selfincompatible (SI) reaction in the Rosaceae. The pistil and pollen determinants of S-specificity in the Rosaceae are a ribonuclease and an F-box protein, respectively. From allele-specific sequence polymorphism of S-specificity genes, molecular S-genotyping systems have been established in various rosaceous fruit tree species. Molecular characterization of S-determinants in self-compatible (SC) cultivars has revealed defects in the S-determinants that cause SC and enabled the development of molecular markers for SC. Marker-assisted selection of SC offspring is now being successfully incorporated into breeding programs. In this paper, we summarize the current knowledge of the molecular basis of self-(in)compatibility, S-genotyping methods, and molecular markers for SC in rosaceous fruit tree species.

show abstract

Section: Subgenus Cerasus (Cherries) 1) Identification Of S-rnase Allmentioning

confidence: 99%

Molecular Basis of Self-(in)compatibility and Current Status of S-genotyping in Rosaceous Fruit Trees

Yamane

Tao

2009

J. Japan. Soc. Hort. Sci.

View full text Add to dashboard Cite

show abstract

“…A maioria das cultivares de P. salicina são autoinférteis, devido ao sistema de autoincompatibilidade gametofítica, determinado pela presença de um loco multialélico, contendo os denominados alelos-S, que codificam para enzimas que interrompem a formação do tubo polínico no processo de polinização (KAUFMANN et al, 1992;NEWBIGIN et al, 1993;BOSKOVIC;TOBUTT, 1996;DE NETTANCOURT, 1997, 2000SAPIR et al, 2004). Como resultado, existe a necessidade do uso de outras cultivares de ameixeira, que servirão como polinizadoras das cultivares produtoras, possibilitando a fecundação, frutificação e boa produção de frutos.…”

Section: Introductionunclassified

Caracterização molecular dos alelos-S de incompatibilidade gametofítica em Prunus salicina Lindl.

et al. 2010

View full text Add to dashboard Cite

RESUMO-Pomares comerciais de ameixeira-japonesa devem conter pelo menos duas cultivares para obter boa fertilização, devido à presença do sistema de incompatibilidade gametofítica, que inibe a autofecundação da grande maioria das cultivares. No presente trabalho, buscou-se identificar e caracterizar molecularmente os alelos-S de 11 cultivares de ameixeira-japonesa (Prunus salicina Lindl.) e verificar a compatibilidade entre os genótipos avaliados. As cultivares Santa Rosa, Santa Rita, Reubennel, Pluma 7, América, Rosa Mineira, Amarelinha, The First, Gulfblaze (Clone São Paulo), Gulfblaze (Clone Guaiba) e Harry Pickstone foram analisadas por meio de Reação em Cadeia da Polimerase (PCR) com três pares de iniciadores específicos para alelos-S. As condições da PCR utilizadas, bem como as combinações de iniciadores, permitiram a identificação de alelos-S nas cultivares de P. salicina estudadas, bem como a indicação dos polinizadores mais compatíveis com algumas das principais cultivares utilizadas na produção de frutas. O sequenciamento de alguns dos alelos-S amplificados revelou elevada similaridade com sequências de nucleotídeos já identificados em outros trabalhos com Prunus spp.. Entretanto, a obtenção de sequências completas de maior número de alelos-S faz-se necessária para o estabelecimento de uma relação de identidade precisa entre os mesmos. Termos para Indexação: Ameixeira-japonesa, autoincompatibilidade gametofítica, PCR, alelos-S. MOLECULAR IDENTIFICATION OF S-ALLELES OF GAMETOPHYTIC INCOMPATIBILITY IN Prunus salicina Lindl.ABSTRACT -Commercial plum orchards must contain at least two cultivars, in order to ensure good fruit set and thus high economic yields, because its carries the gametophytic incompatibility system, that inhibits the self-pollination in a great number of cultivars. The aim of this work was to identify by PCR (Polymerase Chain Reaction) the S-alleles related to gametophytic incompatibility in eleven Prunus salicina (Lindl.) cultivars. The Santa Rosa, Santa Rita, Reubennel, Pluma 7, America, Rosa Mineira, Amarelinha, The First, Gulfblaze (Clone São Paulo), Gulfblaze (Clone Guaíba) e Harry Pickstone cultivars were analysed using three pairs of specific primers for amplifying the S-alleles. The PCR conditions and primers set allowed a characterization of S-alleles in the japonese plum and the identification of the most compatible pollinators to some commercial cultivars. Sequencing analysis of some amplified S-alleles showed high similarity to nucleotides sequences already identified in other studies with Prunus spp.. However, complete sequences of a large number of S-alleles it is necessary to get a more precisely identity of relationship among this S-alleles.

show abstract

“…Studies with RAPD and SCAR markers revealed that only the marker OPI07 750 , closely linked to the S 2 allele, was useful for marker-assisted selection of individuals with the S 2 allele [27]. In apple and almond, with gametophytic incompatibility, S-allele genotyping can be performed using a protein-based method that looks at differences in S-RNase migration through a protein gel [28] or by PCR amplification of genomic DNA with "allele-specific" primers [29,30]. Van Nerum et al [31] reexamined the self-incompatibility genotypes of apple cultivars containing putative "new" S-alleles using S-allelespecific PCR and sequence analysis.…”

Section: Discussionmentioning

confidence: 99%

Pollen Incompatibility in Portuguese Hazelnut Landraces

Martins¹,

Rovira²,

Silva³

et al. 2012

Acta Hortic.

View full text Add to dashboard Cite

In many higher plants, selffertilization and genetically related individuals are prevented by pollen-stigma incompatibility. In the genus Corylus, incompatibility is of the sporophytic type and controlled by a single locus with multiple alleles. The objective of this study is to identify the S-alleles present in a collection of Portuguese landraces in order to select the most appropriate landraces for establishment of future orchards and for breeding programmes. Ten major Portuguese hazelnut landraces were submitted to controlled pollinations in the field, with 18 genotypes whose S-alleles are known. The pollen tubes were observed at 100X under a florescence microscope to evaluate their development. Three landraces were revealed to have S 2 allele, two have S 5 , and four have one of the S 3 , S 5 , S 10 , and S 18 alleles. One landrace was compatible with the 18 S-alleles tested and for two landraces, it was possible to identify both alleles. The information of the self-incompatibility relationship between these old cultivars is obviously useful for selecting the most suitable pollinators for planning new orchards and for new cultivars development.

show abstract

Correlation of stylar ribonuclease zymograms with incompatibility alleles in sweet cherry

Cited by 171 publications

References 8 publications

Molecular Basis of Self-(in)compatibility and Current Status of S-genotyping in Rosaceous Fruit Trees

Molecular Basis of Self-(in)compatibility and Current Status of S-genotyping in Rosaceous Fruit Trees

Caracterização molecular dos alelos-S de incompatibilidade gametofítica em Prunus salicina Lindl.

Pollen Incompatibility in Portuguese Hazelnut Landraces

Contact Info

Product

Resources

About