Correlation of μXRF and LA-ICP-MS in the analysis of a human bone-cartilage sample

Turyanskaya, Anna; Smetaczek, Stefan; Pichler, Vanessa; Rauwolf, Mirjam; Perneczky, Lukas; Roschger, Andreas; Roschger, Paul; Wobrauschek, P.; Limbeck, Andreas; Streli, Christina

doi:10.1039/d1ja00007a

Cited by 13 publications

(4 citation statements)

References 37 publications

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“…Deliver ablated particles to the ICP‒MS instrument’s secondary excitation source for digestion and ionization. then analyze the plasma torch’s excited ions by mass spectrometry 25 μm Quantitative measurement that detects elements spatially Relatively low accuracy and spatial resolution Ca, P, S, Zn, Na, Mg, K 106 , 181 Reflects the spatial expression of elemental distribution in the OA tissue SR-μXRF Utilizing synchrotron radiation as an excitation source. An inner shell electron of the atom is struck and ejected by an X-ray.…”

Section: Different Scales and Different Techniques For Deep Spatial P...mentioning

confidence: 99%

Spatial analysis of the osteoarthritis microenvironment: techniques, insights, and applications

Fan,

Sun,

Young

et al. 2024

Bone Res

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Osteoarthritis (OA) is a debilitating degenerative disease affecting multiple joint tissues, including cartilage, bone, synovium, and adipose tissues. OA presents diverse clinical phenotypes and distinct molecular endotypes, including inflammatory, metabolic, mechanical, genetic, and synovial variants. Consequently, innovative technologies are needed to support the development of effective diagnostic and precision therapeutic approaches. Traditional analysis of bulk OA tissue extracts has limitations due to technical constraints, causing challenges in the differentiation between various physiological and pathological phenotypes in joint tissues. This issue has led to standardization difficulties and hindered the success of clinical trials. Gaining insights into the spatial variations of the cellular and molecular structures in OA tissues, encompassing DNA, RNA, metabolites, and proteins, as well as their chemical properties, elemental composition, and mechanical attributes, can contribute to a more comprehensive understanding of the disease subtypes. Spatially resolved biology enables biologists to investigate cells within the context of their tissue microenvironment, providing a more holistic view of cellular function. Recent advances in innovative spatial biology techniques now allow intact tissue sections to be examined using various -omics lenses, such as genomics, transcriptomics, proteomics, and metabolomics, with spatial data. This fusion of approaches provides researchers with critical insights into the molecular composition and functions of the cells and tissues at precise spatial coordinates. Furthermore, advanced imaging techniques, including high-resolution microscopy, hyperspectral imaging, and mass spectrometry imaging, enable the visualization and analysis of the spatial distribution of biomolecules, cells, and tissues. Linking these molecular imaging outputs to conventional tissue histology can facilitate a more comprehensive characterization of disease phenotypes. This review summarizes the recent advancements in the molecular imaging modalities and methodologies for in-depth spatial analysis. It explores their applications, challenges, and potential opportunities in the field of OA. Additionally, this review provides a perspective on the potential research directions for these contemporary approaches that can meet the requirements of clinical diagnoses and the establishment of therapeutic targets for OA.

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Section: Different Scales and Different Techniques For Deep Spatial P...mentioning

confidence: 99%

Spatial analysis of the osteoarthritis microenvironment: techniques, insights, and applications

Fan,

Sun,

Young

et al. 2024

Bone Res

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Section: Imaging With Ms X-rays and Libsmentioning

confidence: 99%

“…Turyanskaya and co-workers 165,166 focussed on the analysis of bone by LA-ICP-MS and X-ray techniques for different purposes in two papers. In the rst, 165 a human bone-cartilage sample was used to directly compare LA-ICP-MS and mXRF spectrometry. Quantitative results were obtained for both techniques using the CRM IAEA H-5 (animal bone) and an in-house prepared cartilage standard.…”

Section: Imaging With Ms X-rays and Libsmentioning

confidence: 99%

Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages

Patriarca

Barlow

Cross³

et al. 2022

J. Anal. At. Spectrom.

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“…18 Matrix-matched synthetic hydroxyapatite materials doped with analyte metals were developed to improve quantication of calcied tissues. 15,[19][20][21] However, issues arose from being unable to obtain matched ablation behaviour between the calibration material and the sample, 15 with neither ablation yield or normalised sensitivity reported. Previous hydroxyapatite standards suffered from elemental inhomogeneity with relative standard deviation (RSD) up to 32% due to grain size and poor uptake into the crystal lattice.…”

Section: Introductionmentioning

confidence: 99%

Preparation of matrix-matched standards for the analysis of teeth via laser ablation-inductively coupled plasma-mass spectrometry

et al. 2023

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Correlation of μXRF and LA-ICP-MS in the analysis of a human bone-cartilage sample

Abstract: Determining the local distribution of major, minor and trace elements in biological tissues provides fundamental information on the chemical composition and often allows conclusions on the underlying biochemical mechanisms. Within...

Cited by 13 publications

References 37 publications

Spatial analysis of the osteoarthritis microenvironment: techniques, insights, and applications

Spatial analysis of the osteoarthritis microenvironment: techniques, insights, and applications

Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages

Preparation of matrix-matched standards for the analysis of teeth via laser ablation-inductively coupled plasma-mass spectrometry

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