Correlations between changes in intestinal microbiota composition and performance parameters in broiler chickens

Abstract

Growing male Cobb broiler chickens were fed on diets supplemented with additives reported as able to influence intestinal microbiota composition. The diets used were a balanced commercial diet (no additive), inulin (20 g/kg), fructose caramel (FC, 20 g/kg) and the garlic derivative PTS-O (propyl propane thiosulfonate, 45 and 90 mg/kg diet). The composition of the intestinal microbiota was analysed by qPCR at different points of the intestinal tract, and a number of nutritional parameters were also determined. The relative amounts of bacteroides (bacteroides/total bacteria) in the ileal contents correlated (p < 0.05) positively with faecal NDF, ADF, hemicellulose and cellulose digestibility. The relative amounts of Escherichia-Shigella (Escherichia-Shigella/total bacteria) in the crop contents correlated (p = 0.05) negatively with weight gain of broilers. Faecal N digestibility correlated (p < 0.05) negatively with total bacteria in the ileal contents of chickens. The relative amounts of Escherichia-Shigella (Escherichia-Shigella/total bacteria) in the caecal contents correlated (p = 0.05) negatively with faecal fat digestibility of broilers. Total bacteria in ileal or caecal contents of growing chickens correlated (p < 0.05) negatively with ileal N digestibility. The results here reported suggest that positive or negative correlations can be found between performance parameters and changes in intestinal microbiota composition of growing broiler chickens.

Intestinal microbiota of broilers submitted to feeding restriction and its relationship to hepatic metabolism and fat mass: Fast‐growing strain

“…For example, Clostridium cluster IV counts include butyrate‐producer strains like Faecalibacterium prausnitzii (Rinttilä & Apajalahti, ), which is an energy source for colonocytes, and also affect host metabolism, especially through insulin and leptin pathways (van de Wouw et al, ). The lack of correlation between performance and Clostridium cluster XIV in our study is in discordance with previous reports (Chambers et al, ; Rubio et al, ). The high genetic diversity within the Clostridium cluster XIV group could explain these differences, and should be further investigated.…”

“…An interesting result was that Enterobacteriaceae family copy numbers increased in the energetic restricted broilers. It is known that a high proliferation of enterobacteria is associated with mucosa damage, which impair nutrient absorption in the jejunum and ileum (Rubio et al, ), and with metabolic deteriorations in host via bacterial endotoxins (Fei & Zhao, ). Similar patterns of microbiota changes were found in mice after weaning (Lutgendorff et al, ), and in caecum of broilers feeding a high fibre diet (Walugembe et al, ).…”

The Genus Allium as Poultry Feed Additive: A Review

“…In addition, PTS-O was able to significantly increase and modulate the composition of bifidobacteria in growing broilers; which are considered as excellent candidates of probiotics in broilers [108]. Another study involving PTS-O supplementation indicated negative correlations between relative abundances of Escherichia-Shigella or enterobacteria (crop, ileum and caeca) and growth performance as well as fat digestibility in PTS-O fed broilers [109]. When garlic extract (0.04 or 0.06 g/kg feed) was gavaged to broilers reduced number of E. coli and Staphylococcus aureus in the ileo-caecal digesta and improved nutrient digestibility were observed [110].…”

In vitro effects of inulin and soya bean oligosaccharide on skatole production and the intestinal microbiota in broilers

“…It was shown that skatole production was decreased in the large intestine of pigs by the feed added NDC (Jensen & Hansen, 2006;Lösel, Lacorn, Büttner, & Claus, 2006;Øverland, Kjos, Fauske, Teige, & Sorum, 2011). Non-digestible carbohydrates reaching the large intestine are available for microbial fermentation and affect the microbial community (Knudsen, Hedemann, & Laerke, 2012;Rubio et al, 2015). It has been reported that the prebiotic effects of inulin-derived fructans on humans (Salazar et al, 2015) and broilers (Aead, Ghareeb, & Böhm, 2011;Kareem, Loh, Foo, Akit, & Samsudin, 2016;Pruszynska-Oszmalek et al, 2015).…”