Correlations between Histological and Array Comparative Genomic Hybridization Characterizations of Wilms Tumor

Chiang, Ming-Ru; Kuo, Chi-Wen; Wang, Wen Chung; Hou, Tai-Cheng; Kuo, Chen‐Yun; Lu, Meng‐Yao; Lai, Yen‐Chein

doi:10.1007/s12253-019-00601-8

Cited by 6 publications

(9 citation statements)

References 33 publications

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“…The 6 subjects have been described elsewhere 11–13 . Their paraffin‐embedded Wilms' tumour tissue samples were provided by the Department of Pediatrics of National Taiwan University Hospital.…”

Section: Methodsmentioning

confidence: 99%

Correlations between histological characterizations and methylation statuses of tumour suppressor genes in Wilms' tumours

Lai

Wang

et al. 2022

Int J Experimental Path

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Wilms' tumour, also known as nephroblastoma, is an embryonal malignant tumour of the kidney. It is the most commonly occurring solid tumour in children, excluding brain tumours. [1][2][3] The aetiology of Wilms' tumour is still poorly understood, although a number of associated genes and loci have been identified. 4,5 The genetic changes in Wilms' tumours are diverse and involve approximately 40 genes. 6 Genes that have been previously implicated include WT1,

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Section: Methodsmentioning

confidence: 99%

Correlations between histological characterizations and methylation statuses of tumour suppressor genes in Wilms' tumours

Lai

Wang

et al. 2022

Int J Experimental Path

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“…Genomic DNA was puri ed from para n-embedded tissues with the DNA FFPE Tissue Kit (Qiagen), according to the manufacturer's instructions and dissolved in 100 µl of TE buffer (10 mM Tris-HCl, pH 8.0, and 1 mM EDTA) as previously described [11,13]. UV-Vis measurements of DNA concentration of each sample were obtained using NanoDrop UV-VIS Spectrophotometer (Thermo Scienti c Nano-Drop 2000c).…”

Section: Dna Extractionmentioning

confidence: 99%

“…The six subjects have been described elsewhere [11][12][13]. Their para n-embedded Wilms tumor tissue samples were provided by the Department of Pediatrics of National Taiwan University Hospital.…”

Section: Study Subjectsmentioning

confidence: 99%

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Identification of Molecular Biomarkers in Taiwanese Patients with Wilms Tumor Using a Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA)-Based Approach

Wang

Hou

et al. 2020

Preprint

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Background: Wilms tumor is a solid tumor that frequently occurs in children. Genetic or epigenetic aberrations in WT1 and WT2 loci are implicated in its etiology. Moreover, tumor suppressor genes are frequently silenced by methylation in this tumor. Methods: In the present study, we analyzed the methylation statuses of promoter regions of 24 different tumor suppressor genes using a methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA)-based approach in six Wilms tumors. Results: All six Wilms tumors showed methylation of RASSF1 specific to tumors, not in normal tissues. Moreover, methylated HIC1 was identified in stromal type Wilms tumors and methylated BRCA1 was identified in epithelial type Wilms tumors. Unmethylated CASP8, RARB, MLH1_167, APC, and CDKN2A were found only in blastemal predominant type Wilms tumors. Conclusions: Our results indicated that methylation of RASSF1 is the essential event in the tumorigenesis of Wilms tumor, which may inform its clinical and therapeutic management. In addition, mixed type Wilms tumors may be classified according to epithelial, stromal, and blastemal components via MS-MLPA-based approach.

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“…Recently, much attention was switched to the roles of SIX1 in tumorigenesis. Reports showed that SIX1 participates in various cancers initiation, including hepatocellular carcinoma ( 7 ), breast cancer ( 8 ), ovarian cancer ( 9 ), cervical cancer ( 10 ), osteosarcoma ( 11 ), colorectal cancer ( 12 ), and Wilms tumors ( 13 ). Meanwhile, SIX1 has also been verified in promoting cancer progression, accelerating cancer cell metabolism and progression.…”

Section: Introductionmentioning

confidence: 99%

The Significance of SIX1 as a Prognostic Biomarker for Survival Outcome in Various Cancer Patients: A Systematic Review and Meta-Analysis

et al. 2021

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Sine Oculis Homeobox Homolog 1 (SIX1) is reported to promote cancer initiation and progression in many preclinical models and is demonstrated in human cancer tissues. However, the correlation between SIX1 and cancer patients’ prognosis has not yet been systematically evaluated. Therefore, we performed a systematic review and meta-analysis in various human cancer types and extracted some data from TCGA datasets for further verification and perfection. We constructed 27 studies and estimated the association between SIX1 expression in various cancer patients’ overall survival and verified with TCGA datasets. Twenty-seven studies with 4899 patients are include in the analysis of overall, and disease-free survival, most of them were retrospective. The pooled hazard ratios (HRs) for overall and disease-free survival in high SIX1 expression patients were 1.54 (95% CI: 1.32-1.80, P<0.00001) and 1.83 (95% CI: 1.31-2.55, P=0.0004) respectively. On subgroup analysis classified in cancer type, high SIX1 expression was associated with poor overall survival in patients with hepatocellular carcinoma (HR 1.50; 95% CI: 1.17-1.93, P =0.001), breast cancer (HR 1.31; 95% CI: 1.10-1.55, P =0.002) and esophageal squamous cell carcinoma (HR 1.89; 95% CI: 1.42-2.52, P<0.0001). Next, we utilized TCGA online datasets, and the consistent results were verified in various cancer types. SIX1 expression indicated its potential to serve as a cancer biomarker and deliver prognostic information in various cancer patients. More works still need to improve the understandings of SIX1 expression and prognosis in different cancer types.

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Correlations between Histological and Array Comparative Genomic Hybridization Characterizations of Wilms Tumor

Cited by 6 publications

References 33 publications

Correlations between histological characterizations and methylation statuses of tumour suppressor genes in Wilms' tumours

Correlations between histological characterizations and methylation statuses of tumour suppressor genes in Wilms' tumours

Identification of Molecular Biomarkers in Taiwanese Patients with Wilms Tumor Using a Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA)-Based Approach

The Significance of SIX1 as a Prognostic Biomarker for Survival Outcome in Various Cancer Patients: A Systematic Review and Meta-Analysis

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