Correlations between microRNA-146a and immunoglobulin and inflammatory factors in Guillain–Barré syndrome

Abstract

To study correlations between expression of microRNA-146a (miR-146a) and immunoglobulin and inflammatory cytokines in serum of patients with Guillain-Barr e syndrome (GBS). Methods: Eighty-four patients with GBS were selected as the experimental group and 50 healthy individuals as controls. Reverse transcription-PCR was used to detect expression of miR-146a in peripheral blood of all participants. Immunoturbidometric assay was used to detect immunoglobulins (Ig)G, IgA, and IgM in peripheral blood of all participants. The levels of interleukin-6 (IL-6), C-reactive protein (CRP), and tumor necrosis factor a (TNF-a) were measured by ELISA in peripheral blood. The expression of miR-146a was compared between the two groups and Pearson correlation analysis was used to analyze correlations between miR-146a and immunoglobulin and inflammatory factors. Results: Expression of miR-146a was higher in the GBS group than in controls. Expression of IL-6, CRP, TNF-a, and IgG was significantly higher in the GBS group than in controls. miR-146a was significantly and positively correlated with IL-6, CRP, TNF-a, and IgG but not with IgA and IgM. Conclusion: The expression profile of miR-146a in patients with GBS differs from that in healthy individuals. Thus, miR-146a may participate in the pathogenesis of GBS by regulating immune and inflammatory responses.

Fulminant Guillain–Barré Syndrome and Spontaneous Intraventricular Hemorrhage: A Case Report and Literature Review

“…Moreover, other inflammatory cytokines, like interferon-γ, IL-4, IL-17, IL-23, and IL-8, are increased after GBS ( Debnath et al, 2018 ; Breville et al, 2019 ; Sun et al, 2019 ). Recent study also reported that the expression of microRNA-146a, which was correlated with IL-6 and TNF-a, was higher in the GBS ( Huang et al, 2020 ). These inflammatory cytokines ultimately induce demyelination, nerve lesions, and axonal degeneration and result in the development of GBS.…”

Expression Analysis of Protein Inhibitor of Activated STAT in Inflammatory Demyelinating Polyradiculoneuropathy

“…Several miRNAs including miR-146a have been shown to affect expression of PIAS, STAT and other components of this pathway ( 26 ). Meanwhile, expression of a number of these miRNAs including miR-146a has been found to be dysregulated in GBS patients ( 27 ). Besides, miR-18a has been found to negatively regulate PIAS3 expression and therefore influencing expression of STAT3 target genes ( 28 ).…”

Network pharmacology analysis of the mechanism of our hospital’s experiential prescription in the treatment of Guillain Barré syndrome

“…For example, the relationship between TNF and GBS has been reported in a number of studies, showing that it is highly related to the inflammatory response of GBS, and there have been case reports claiming that anti-TNF therapy may trigger the occurrence of GBS (22). All types of GBS have been reported to have increased serum levels of TNF-α (23,24). There are some evidences that EGFR and ITGAL are directly involved in GBS; in particular, the EGFR signal plays an important role in demyelination and remyelination e, which may underpin a part of the mechanism of GBS development.…”