Correlations between platelet-derived microvesicles and thrombin generation in patients with coronary artery disease

Soßdorf, Maik; Konig, Victoria; Gummert, Jan; Marx, Gernot; Lösche, Wolfgang

doi:10.1080/09537100802210337

Cited by 9 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It measures the rate of thrombin generation and inhibition in citrated plasma, and has been used to quantify procoagulant activity in several diseases including VTE and coronary artery disease. 16-18 In our previous pilot study, we performed native thrombin generation assays, as initially described by Dunbar et al 19 , in which no agonist such as tissue factor or phospholipid source (PCPS) were added. The role of CAT in clinical practice has yet to be defined largely due to lack of official standardization of the assay with its associated large interlaboratory variability.…”

Section: Discussionmentioning

confidence: 99%

Thrombin generation and procoagulant microparticle profiles after acute trauma

Park

Xue

Spears

et al. 2015

Journal of Trauma and Acute Care Surgery

View full text Add to dashboard Cite

Objective The two sides of Trauma Induced Coagulopathy (TIC), the hypo- and the hyper- coagulable states, are poorly understood. To identify potential mechanisms for venous thromboembolism and bleeding after acute trauma, we estimated changes in circulating procoagulant MPs and thrombin activity during hospitalization for trauma. Methods Whole blood was collected by venipuncture into 3.2% trisodium citrate at 0, 6, 12, 24 and 72 hours after injury, and discharge. Platelet poor plasma was harvested and stored at −80°C until analysis. Thrombin generation was determined using the calibrated automated thrombogram (CAT), reported as lagtime (minutes), peak height (nM thrombin) and time to reach peak height (ttPeak - minutes). The concentration of total procoagulant MPs (number/uL]) was measured by flow cytometry. Data are presented as median [interquartile range]. Results Among 443 trauma patients (1734 samples; ISS=13.0 [6.0, 22.0], hospital LOS=4.0 [2.0, 10.0] days, age=48 [28, 65] years, 70.7% male, 95% with blunt mechanism, mortality 3.2%), no discernable patterns in thrombin generation or MP concentration were observed over time. The peak height and MPs were significantly different from healthy volunteers and were 337 [285, 395] nM and 400 [211, 772] per uL plasma, respectively. Extreme (defined as highest or lowest 5%) values reflecting a possible “hypercoagulable state” (lagtime ≤ 1.98, peak height ≥ 486.2, ttPeak ≤ 3.61, and total procoagulant MP ≥ 2278) were reached within 12 hours after acute trauma, while extreme values representing a possible “hypocoagulable state” (lagtime ≥ 18.6, peak height ≤ 17.8 and ttPeak ≥ 29.45) were not reached until 1-3 days. Conclusion Although there was no predictable pattern of coagulopathy observed in each patient after trauma, those who reached extreme values did so relatively early after injury. These findings should be taken into account when designing risk model tools involving coagulation laboratory parameters.

show abstract

Section: Discussionmentioning

confidence: 99%

Thrombin generation and procoagulant microparticle profiles after acute trauma

Park

Xue

Spears

et al. 2015

Journal of Trauma and Acute Care Surgery

View full text Add to dashboard Cite

show abstract

“…Our data on MP and their procoagulant activity after moderate exercise may support the phenomenon of an exercise-induced increase in the haemostatic potential. In a previous study with coronary artery diseased patients we observed positive correlations between the number of circulating PMP and thrombin generation [18]. Numerous epidemiological and prospective cohort studies confirm an important beneficial role of moderate endurance exercise in the prevention and treatment of cardiovascular diseases [9].…”

mentioning

confidence: 96%

Release of pro-coagulant microparticles after moderate endurance exercise

et al. 2010

View full text Add to dashboard Cite

“…First is the Calibrated Automated Thrombogram (CAT), a plate-based assay that measures the rate of thrombin generation and inhibition in citrated plasma, and has been used to quantify procoagulant activity in several diseases including VTE and coronary artery disease. [8][9][10] The clinical utility of in vitro thrombin generation however, remains to be determined in trauma patients. The objective of this study was to characterize and quantify procoagulant activity and MP in plasma obtained from individuals after traumatic injury.…”

Section: Discussionmentioning

confidence: 99%

Microvesicle Production After Trauma and Its Clinical Impact on Venothromboembolism

Park¹

2011

View full text Add to dashboard Cite

Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS.

show abstract

Correlations between platelet-derived microvesicles and thrombin generation in patients with coronary artery disease

Cited by 9 publications

References 8 publications

Thrombin generation and procoagulant microparticle profiles after acute trauma

Thrombin generation and procoagulant microparticle profiles after acute trauma

Release of pro-coagulant microparticles after moderate endurance exercise

Microvesicle Production After Trauma and Its Clinical Impact on Venothromboembolism

Contact Info

Product

Resources

About