Correlations of follicular fluid oxidative stress biomarkers and enzyme activities with embryo morphology parameters during in vitro fertilization

Fujimoto, Victor Y.; Bloom, Michael S.; Huddleston, Heather G.; Shelley, Wendy B.; Ocque, Andrew J.; Browne, Richard W.

doi:10.1016/j.fertnstert.2011.09.032

Cited by 37 publications

(28 citation statements)

References 44 publications

(64 reference statements)

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“…Eicosanoid pathways, either through COX or LOX, add molecular oxygen that generates reactive oxygen species [39,40]. Depletion of the scavenging capacity of antioxidant defense systems is hazardous to cells and leads to oxidative damage.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, in view of confusing data, a recent robust study by Fujimoto et al evaluated the relevance of a vast panel of antioxidant enzyme activities and specific lipid peroxidation end products by correlational analyses with early embryo morphology parameters, embryo fragmentation, and embryo cell number, using a 1 follicle—1 oocyte/embryo design, analogous to our study [39]. Although the authors did not find correlations between lipid peroxidation derivatives or antioxidant enzyme activities and embryo quality, post hoc power analysis indicated possible undetected associations between the embryo fragmentation score and 13-hydroxy octadecatrienoic acid (13-HOTE) and 13-hydroperoxy octadecadieneoic acid (13-HPODE).…”

Section: Discussionmentioning

confidence: 99%

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Arachidonic and Linoleic Acid Derivatives Impact Oocyte ICSI Fertilization – A Prospective Analysis of Follicular Fluid and a Matched Oocyte in a ‘One Follicle – One Retrieved Oocyte – One Resulting Embryo’ Investigational Setting

et al. 2015

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ObjectiveTo evaluate human oocyte ability to undergo fertilization and subsequent preimplantation embryonic development in relation to a wide panel of follicular fluid (FF) arachidonic acid derivatives (AAD) and linoleic acid derivatives (LAD) of prospectively selected patients undergoing intracytoplasmic sperm injection (ICSI).MethodologyStudy was designed as a two center (a university clinic and a private clinic) prospective study. 54 women of 181 consecutive couples undergoing ICSI were prospectively found to be eligible for analysis. 'One follicle – one retrieved oocyte – one resulting embryo' approach was used. Each individual follicle was aspirated independently and matched to an oocyte growing in this particular follicular milieu. FF samples were assessed for AAD and LAD by high-performance liquid chromatography; additionally, activity of secretory phospholipase A (sPLA2) was determined by enzyme-linked immunosorbent assay.Principal FindingsIncreased activity of sPLA2 and significantly higher AAD and LAD levels were found in FF of oocytes that did not show two pronuclei or underwent degeneration after ICSI in comparison to oocytes with the appearance of two pronuclei. Receiver operating characteristics curve analysis identified acids with the highest sensitivity and specificity: 5oxo-hydroxyeicosatetraenoic, 16-hydroxyeicosatetraenoic, 9-hydroxyoctadecadieneoic and 12-hydroxyeicosatetraenoic. No significant differences between AAD and LAD related to embryo quality were found.Conclusions/SignificanceOur study demonstrates for the first time that elevated concentrations of AAD and LAD in FF at the time of oocyte retrieval significantly decrease the ability of oocytes to form pronuclei after ICSI. This may serve as a new tool for non-invasive assessment of oocyte developmental capacity. However, levels of AAD and LAD are not associated with subsequent embryo quality or pregnancy rate, and therefore more studies are needed to determine their usefulness in human IVF procedure.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Arachidonic and Linoleic Acid Derivatives Impact Oocyte ICSI Fertilization – A Prospective Analysis of Follicular Fluid and a Matched Oocyte in a ‘One Follicle – One Retrieved Oocyte – One Resulting Embryo’ Investigational Setting

et al. 2015

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“…Reactive species are commonly generated as part of normal cellular metabolism or as a consequence of exposure to exogenous toxic molecules or stressful processes [12]. In addition to their destructive actions, these species play important roles as second messengers in cellular functions through the activation of cell signaling cascades, such as those involving mitogen-activated protein kinases and regulation of transcription factors [13].…”

Section: Reactive Species and Melatoninmentioning

confidence: 99%

Essential actions of melatonin in protecting the ovary from oxidative damage

Cruz¹,

Leal²,

Cruz

et al. 2014

Theriogenology

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“…Investigators have characterized endogenous human FF constituents including hormones, proteins, reactive oxygen species, amino acids and sugars, as well as dissolved O 2 (5). Several groups described concentrations of HDL-particle associated lipids, proteins, micronutrients and enzyme activities in the human ovarian follicle (3, 4, 6–10). Interest in the use of FF as a source of biomarkers predictive of IVF outcomes has gained tremendous momentum, although with limited success to date (5, 11).…”

Section: Introductionmentioning

confidence: 99%

Variability in the components of high-density lipoprotein particles measured in human ovarian follicular fluid: a cross-sectional analysis

Bloom

Kim

Fujimoto

et al. 2014

Fertility and Sterility

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Objective Assess the variability of follicular fluid (FF) high-density lipoprotein (HDL) particle components. Design FF specimens were collected from two contralateral follicles on the day of oocyte retrieval and analyzed for HDL components. We characterized analytes by age, body mass index (BMI), race and smoking using a cross-sectional design. Biological variability was assessed using two-stage nested analysis of variance. Setting Reproductive health center. Patients One-hundred eighty in vitro fertilization (IVF) patients. Interventions None. Main Outcome Measures Nineteen HDL components including HDL-cholesterol and free (unesterified) and esterified forms, phospholipids, triglycerides, apolipoproteins A-1 and A-2, paraoxonase 1 (PON1) activities, and seven lipophilic vitamins and micronutrients. Results For some analytes, a majority of total measurement variability was attributed to sources between-follicles, suggesting an important role for the integrity of the blood-follicle-barrier and in situ remodeling of plasma derived constituents. For other analytes, variability was mostly attributed to sources between-women, likely indicative of plasma levels. Variability between-follicles decreased with increasing age, differed by BMI and smoking, and generally were lower for Asians and women with diminished ovarian reserve. Conclusions Substantial variability in FF HDL components exist between-follicles among women undergoing IVF, as well as between-women by age, BMI, race, smoking and by infertility diagnosis.

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Correlations of follicular fluid oxidative stress biomarkers and enzyme activities with embryo morphology parameters during in vitro fertilization

Cited by 37 publications

References 44 publications

Arachidonic and Linoleic Acid Derivatives Impact Oocyte ICSI Fertilization – A Prospective Analysis of Follicular Fluid and a Matched Oocyte in a ‘One Follicle – One Retrieved Oocyte – One Resulting Embryo’ Investigational Setting

Arachidonic and Linoleic Acid Derivatives Impact Oocyte ICSI Fertilization – A Prospective Analysis of Follicular Fluid and a Matched Oocyte in a ‘One Follicle – One Retrieved Oocyte – One Resulting Embryo’ Investigational Setting

Essential actions of melatonin in protecting the ovary from oxidative damage

Variability in the components of high-density lipoprotein particles measured in human ovarian follicular fluid: a cross-sectional analysis

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