Correlations of IL-17 and NF-κB gene polymorphisms with susceptibility and prognosis in acute respiratory distress syndrome in a chinese population

Abstract

The present study was performed to investigate the association between interleukin-17 (IL-17) and nuclear factor κB (NF-κB) gene polymorphisms and the risk and prognosis of acute respiratory distress syndrome (ARDS) in a Chinese population. A total of 210 Chinese patients with ARDS were selected as the study group, 210 individuals who were identified as at-risk patients but did not meet criteria for ARDS were recruited as the control group. Three single nucleotide polymorphisms (SNPs) of IL-17, including rs763780 (A>G), rs2275913 (G>A), rs8193036 (C>T) and NF-κB1 gene rs3774934 (G>A) loci were examined by Sanger sequencing technique in the peripheral blood of all subjects. Patients were followed for 30-day survival. The IL-17 rs763780 and NF-κB1 rs3774934 SNPs had no impact on ARDS risk and prognosis of ARDS (P>0.05). Compared with individuals carrying the wild-type GG genotype of rs2275913 at IL-17, the AA-homozygous and GA- heterozygous individuals were protected from the development of ARDS. Consistently, a decreased 30-day mortality risk was found among A-allele carriers of rs2275913 at IL-17 (p<0.05). For IL-17 rs8193036 SNP, the homozygote TT genotype and heterozygote CT genotypes were associated with increased ARDS susceptibility and 30-day mortality risk (P<0.05). Besides, decreased IL-17 levels were found in A-allele carriers of IL-17 rs2275913, whereas individuals carrying T-allele of IL-17 rs8193036 were found to have significantly increased levels of IL-17 (P<0.05). Our results suggested that two functional polymorphisms of IL-17, rs2275913 and rs8193036 were associated with ARDS risk and prognosis, indicating that the two genetic variants might act as possible markers for the prediction of ARDS risk and development.

Genetic polymorphisms in PXR and NF-κB1 influence susceptibility to anti-tuberculosis drug-induced liver injury

“…Therefore, the NF-κB1 gene is one of the key genes in the NF-κB signalling pathway. There have been many studies on the association of genetic polymorphisms of the NF-κB1 gene with immune-related diseases such as inflammatory bowel disease[24], infectious diseases[25] and tumours[17, 23, 26]. There is evidence that NF-κB1 may directly interact with the DNA-binding domain of PXR to suppress its expression and inhibit its transactivation[27].…”

From sepsis to acute respiratory distress syndrome (ARDS): emerging preventive strategies based on molecular and genetic researches

“…The release of many cytokine and the regulation of inflammatory pathways are regulated by some transcription factors such as NF-κB, thus, any changes in their structure and expression might influence cytokine production and ARDS progress [61]. Since increased IL-17 may serve as a biomarker to indicate the severity of ARDS in patients with sepsis-induced ARDS [62], researchers have estimated the IL-17 (rs763780, rs2275913, and rs8193036) and NF-κB1 (rs3774934) polymorphisms in a Chinese population that were associated with ARDS prediction and prognosis [63]. This conclusion further shows that the detection of related gene expression and variants has an important role in the prediction and prevention of the occurrence and development of ARDS.…”

Correlations of IL-6, IL-6R, IL-10 and IL-17 gene polymorphisms with the prevalence of COVID-2019 infection and its mortality rate&nbsp;

“…63,64 A retrospective analysis of IL-17 gene polymorphisms in patients with acute respiratory distress syndrome (ARDS) revealed that patients with a polymorphism that resulted in attenuated IL-17 production had an increased 30-day survival, whereas a genetic polymorphism that resulted in producing more IL-17 correlated with decreased survival. 65 Mikacenic et al measured circulating IL-17A in ARDS and showed that elevated circulating and alveolar levels of IL-17A are associated with increased percentage of alveolar neutrophils, alveolar permeability and organ dysfunction in ARDS. 66 However, there is no detailed information about the association between frequency of polymorphisms of IL-17A and IL-17F genes of COVID patients among the populations.…”