Correspondence: Revisiting the theoretical cell membrane thermal capacitance response

Plaksin, Michael; Kimmel, Eitan; Shoham, Sharon

doi:10.1038/s41467-017-00435-5

Cited by 29 publications

(23 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5(a)), as would be typically expected from the TRPV channels [41,42], this class of ion channels is unlikely to play a role in the results shown here. Further, it has been suggested that IR irradiation of the cell membrane can generate capacitive currents which in turn can influence the membrane potential [10][11][12][13]. However, membrane potential changes associated with IR mediated capacitive currents typically exhibit a rapid rising phase at the onset of the IR irradiation, which was not observed here ( Fig.…”

Section: Mechanisms Underlying Infrared Induced Inhibition and Actionmentioning

confidence: 55%

“…It has been suggested that thermal transients induced by water absorption of pulsed IR light could be the underlying mechanism for neuronal excitation [9]. Changes in the membrane capacitance caused by spatiotemporal temperature gradients have been demonstrated to depolarize cells and elicit action potentials [10][11][12][13]. Pulsed IR light irradiation has also been shown to modulate intracellular calcium dynamics in neonatal ventricular cardiomyocytes [14], in astrocytes and neurons in vivo [15], and in spiral and vestibular ganglion neurons [16].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Infrared inhibition and waveform modulation of action potentials in the crayfish motor axon

Zhu¹,

Lin²,

Sander³

2019

Biomed. Opt. Express

View full text Add to dashboard Cite

The infrared (IR) inhibition of axonal activities in the crayfish neuromuscular preparation is studied using 2 µm IR light pulses with varying durations. The intracellular neuronal activities are monitored with two-electrode current clamp, while the IR-induced temperature changes are measured by the open patch technique simultaneously. It is demonstrated that the IR pulses can reversibly shape or block locally initiated action potentials. Suppression of the AP amplitude and duration and decrease in axonal excitability by IR pulses are quantitatively analyzed. While the AP amplitude and duration decrease similarly during IR illumination, it is discovered that the recovery of the AP duration after the IR pulses is slower than that of the AP amplitude. An IR-induced decrease in the input resistance (8.8%) is detected and discussed together with the temperature dependent changes in channel kinetics as contributing factors for the inhibition reported here.

show abstract

Section: Mechanisms Underlying Infrared Induced Inhibition and Actionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Infrared inhibition and waveform modulation of action potentials in the crayfish motor axon

Zhu¹,

Lin²,

Sander³

2019

Biomed. Opt. Express

View full text Add to dashboard Cite

show abstract

“…Although the pioneering study by Shapiro et al [16] also sought to unravel their results' underlying biophysical mechanism, upon further scrutiny we found that their fixed-geometry GCS peripheral capacitances (bulk and Stern) decrease with temperature [28,29]. Our new model leads to a diametrically different qualitative story, where the underlying phenomenon is now instead attributed to the following two completely novel mechanisms.…”

Section: Discussionmentioning

confidence: 88%

Thermal Transients Excite Neurons through Universal Intramembrane Mechanoelectrical Effects

et al. 2018

Self Cite

View full text Add to dashboard Cite

Modern advances in neurotechnology rely on effectively harnessing physical tools and insights towards remote neural control, thereby creating major new scientific and therapeutic opportunities. Specifically, rapid temperature pulses were shown to increase membrane capacitance, causing capacitive currents that explain neural excitation, but the underlying biophysics is not well understood. Here, we show that an intramembrane thermal-mechanical effect wherein the phospholipid bilayer undergoes axial narrowing and lateral expansion accurately predicts a potentially universal thermal capacitance increase rate of ∼0.3%=°C. This capacitance increase and concurrent changes in the surface charge related fields lead to predictable exciting ionic displacement currents. The new MechanoElectrical Thermal Activation theory's predictions provide an excellent agreement with multiple experimental results and indirect estimates of latent biophysical quantities. Our results further highlight the role of electro-mechanics in neural excitation; they may also help illuminate subthreshold and novel physical cellular effects, and could potentially lead to advanced new methods for neural control.

show abstract

“…These coupled equations describe the relation between the mobile (Q) and fixed (

) membrane charge densities and the potential drops of the bulk regions (

). We used the notation according to Plaksin and co-workers ( Plaksin et al, 2017a , Plaksin et al, 2017b ).

2

2

…”

Section: Methodsmentioning

confidence: 99%

A label-free approach to detect ligand binding to cell surface proteins in real time

Burtscher

Hoťka

et al. 2018

eLife

View full text Add to dashboard Cite

Electrophysiological recordings allow for monitoring the operation of proteins with high temporal resolution down to the single molecule level. This technique has been exploited to track either ion flow arising from channel opening or the synchronized movement of charged residues and/or ions within the membrane electric field. Here, we describe a novel type of current by using the serotonin transporter (SERT) as a model. We examined transient currents elicited on rapid application of specific SERT inhibitors. Our analysis shows that these currents originate from ligand binding and not from a long-range conformational change. The Gouy-Chapman model predicts that adsorption of charged ligands to surface proteins must produce displacement currents and related apparent changes in membrane capacitance. Here we verified these predictions with SERT. Our observations demonstrate that ligand binding to a protein can be monitored in real time and in a label-free manner by recording the membrane capacitance.

show abstract

Correspondence: Revisiting the theoretical cell membrane thermal capacitance response

Cited by 29 publications

References 12 publications

Infrared inhibition and waveform modulation of action potentials in the crayfish motor axon

Infrared inhibition and waveform modulation of action potentials in the crayfish motor axon

Thermal Transients Excite Neurons through Universal Intramembrane Mechanoelectrical Effects

A label-free approach to detect ligand binding to cell surface proteins in real time

Contact Info

Product

Resources

About