Corrigendum: Significant improvement of intestinal microbiota of gibel carp (<i>Carassius auratus gibelio</i>
) after traditional Chinese medicine feeding

Wu, Zhenbing; Gatesoupe, François-Joël; Li, T.T.; Wang, X.H.; Zhang, Q.Q.; Feng, Dongyue; Feng, Yuqing; Chen, H.; Li, A.H.

doi:10.1111/jam.13893

Cited by 6 publications

(3 citation statements)

References 1 publication

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“…The microbial communities present in fish rearing water are thought to affect the initial colonization of the fish microbiota during development (Llewellyn et al, 2014;Talwar et al, 2018). However, similar to other studies (Uren Webster et al, 2018;Wu et al, 2018), our fish gut microbiomes were distinct from the water sample microbiomes. This indicates that the fish host gut microbiome is likely largely independent of the water microbial community and that other factors such as diet and host genome may be contributing disproportionally (Talwar et al, 2018).…”

Section: Discussionsupporting

confidence: 81%

Regulation of Host Gene Expression by Gastrointestinal Tract Microbiota in Chinook Salmon (Oncorhynchus tshawytscha)

Sadeghi

Chaganti

Heath

2022

Preprint

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Differences in gut microbiome composition are linked with health, disease and ultimately host fitness; however, the molecular mechanisms underlying that relationship are not well characterized. Here, we modified the fish gut microbiota using antibiotic and probiotic feed treatments to address the effect of host microbiome on gene expression patterns. Chinook salmon (Oncorhynchus tshawytscha) gut gene expression was evaluated using whole transcriptome sequencing (RNA-Seq) on hindgut mucosa samples from individuals treated with antibiotic, probiotic and control diets to determine differentially expressed (DE) host genes. Fifty DE host genes were selected for further characterization using nanofluidic qPCR chips. We used 16S rRNA gene metabarcoding to characterize the rearing water and host gut microbiome bacterial communities. Daily administration of antibiotics and probiotics resulted in significant changes in fish gut and aquatic microbiota as well as more than 100 DE genes in the antibiotic and probiotic treatment fish, relative to healthy controls. Normal microbiota depletion by antibiotics mostly led to downregulation of different aspects of immunity and upregulation of apoptotic process. In the probiotic treatment, genes related to post-translation modification and inflammatory responses were up-regulated relative to controls. Our qPCR results revealed significant effects of treatment (antibiotic and probiotic) on rabep2, aifm3, manf, prmt3 gene transcription. Moreover, we found significant associations between members of Lactobacillaceae and Aeromonadaceae with host gene expression patterns. Overall, our analysis showed that the microbiota had significant impacts on many host signaling pathways, specifically targeting immune, developmental, and metabolic processes. Our characterization of some of the molecular mechanisms involved in microbiome-host interactions will help develop new strategies for preventing/ treating microbiome disruption-related diseases.

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Section: Discussionsupporting

confidence: 81%

Regulation of Host Gene Expression by Gastrointestinal Tract Microbiota in Chinook Salmon (Oncorhynchus tshawytscha)

Sadeghi

Chaganti

Heath

2022

Preprint

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“…The major histocompatibility complex (MHC) molecules play a central role in adaptive immunity. They are immunoglobulin super family member proteins that interact with T-cells through a specific T-cell receptor (TCR) in order to initiate immune responses [46] and both types of MH receptors (MHC I and II) are present in teleosts [47]. Following vaccination in this study, the expression of MHCI was clearly expressed in the kidney and spleen, while a minimal expression was observed in intestine tissues, indicating a powerful initiation of cellular immune response upon recognition of the recombinant cell vaccines at the early stage of vaccination.…”

Section: Discussionmentioning

confidence: 99%

Elucidating the Efficacy of Vaccination against Vibriosis in Lates calcarifer Using Two Recombinant Protein Vaccines Containing the Outer Membrane Protein K (r-OmpK) of Vibrio alginolyticus and the DNA Chaperone J (r-DnaJ) of Vibrio harveyi

et al. 2020

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Recombinant cell vaccines expressing the OmpK and DnaJ of Vibrio were developed and subsequently, a vaccination efficacy trial was carried out on juvenile seabass (~5 cm; ~20 g). The fish were divided into 5 groups of 50 fish per group, kept in triplicate. Groups 1 and 2 were injected with 107 CFU/mL of the inactivated recombinant cells vaccines, the pET-32/LIC-OmpK and pET-32/LIC-DnaJ, respectively. Group 3 was similarly injected with 107 CFU/mL of inactivated E. coli BL21 (DE3), Group 4 with 107 CFU/mL of formalin killed whole cells V. harveyi, and Group 5 with PBS solution. Serum, mucus, and gut lavage were used to determine the antibody levels before all fish were challenged with V. harveyi, V. alginolyticus, and V. parahemolyticus, respectively on day 15 post-vaccination. There was significant increase in the serum and gut lavage antibody titers in the juvenile seabass vaccinated with r-OmpK vaccine. In addition, there was an up-regulation for TLR2, MyD88, and MHCI genes in the kidney and intestinal tissues of r-OmpK vaccinated fish. At the same time, r-OmpK triggered higher expression level of interleukin IL-10, IL-8, IL-1ß in the spleen, intestine, and kidney compared to r-DnaJ. Overall, r-OmpK and r-DnaJ triggered protection by curbing inflammation and strengthening the adaptive immune response. Vaccinated fish also demonstrated strong cross protection against heterologous of Vibrio isolates, the V. harveyi, V. alginolyticus, and V. parahaemolyticus. The fish vaccinated with r-OmpK protein were completely protected with a relative per cent of survival (RPS) of 90 percent against V. harveyi and 100 percent against V. alginolyticus and V. parahaemolyticus. A semi-quantitative PCR detection of Vibrio spp. from the seawater containing the seabass also revealed that vaccination resulted in reduction of pathogen shedding. In conclusion, our results suggest r-OmpK as a candidate vaccine molecule against multiple Vibrio strain to prevent vibriosis in marine fish.

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“…As an important green natural feed additive, Chinese herbal medicine has been widely used in aquaculture (Abarike et al, 2019; Jian & Wu, 2004; Lin et al, 2016; Mo et al, 2016; Wang, Liu et al, 2019; Wu et al, 2018), and its ability to promote growth, improve feed utilization rate and disease resistance has been confirmed by most experts and scholars. These herbal ingredients include Radix Bupleuri (Jia et al, 2019), Lycium barbarum polysaccharides (Zhang et al, 2020), ginger (Fu et al, 2017), Panax notoginseng (Sun et al, 2018), Sophora flavescens (Wu, Gong et al, 2013), garlic (Ahmadniaye Motlagh, Safari et al, 2020), Chinese yam (Meng et al, 2019), jujube (Liu et al, 2021), Scutellaria baicalensis (Xia et al, 2021), etc.…”

Section: Introductionmentioning

confidence: 99%

Combined effects of jujube, Chinese yam and astragalus on digestion, immunity and intestinal microflora of rainbow trout

Zhang

Liu

Wang

2022

Aquaculture Research

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Rainbow trout (Oncorhynchus mykiss) is an important cold-water economic fish. In recent years, with the continuous expansion of cold-water fish breeding scale, increasing water load and deteriorating water environment (Betiku et al., 2018), aquaculture fish diseases occur frequently (Brodeur et al., 2021), causing significant losses to aquaculture enterprises and individual farmers and affecting the development of cold-water fish industry and rural economy.In order to treat and prevent the occurrence of disease, antibiotics and chemical synthetic additives are generally used at present (Fraslin et al., 2020). But their use can lead not only to drug residues (Liang et al., 2018), but also to drug resistance in fish (Miller & Harbottle, 2018). In addition, after the drug remains in fish, it may be transformed into more toxic substances, which will eventually be transferred to humans through the food chain (Nnadozie & Odume, 2019), resulting in potential threats to human health and public health events (Brodeur et al., 2021). Excessive use of

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Corrigendum: Significant improvement of intestinal microbiota of gibel carp (Carassius auratus gibelio ) after traditional Chinese medicine feeding

Cited by 6 publications

References 1 publication

Regulation of Host Gene Expression by Gastrointestinal Tract Microbiota in Chinook Salmon (Oncorhynchus tshawytscha)

Regulation of Host Gene Expression by Gastrointestinal Tract Microbiota in Chinook Salmon (Oncorhynchus tshawytscha)

Elucidating the Efficacy of Vaccination against Vibriosis in Lates calcarifer Using Two Recombinant Protein Vaccines Containing the Outer Membrane Protein K (r-OmpK) of Vibrio alginolyticus and the DNA Chaperone J (r-DnaJ) of Vibrio harveyi

Combined effects of jujube, Chinese yam and astragalus on digestion, immunity and intestinal microflora of rainbow trout

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