In order to develop the biological self-cleaning anti-clogging high-permeability sand filter tube, it is very important to analyze the plugging material and plugging mechanism of crude oil sand water filter. Under laboratory conditions, based on vacuum drying, condensation reflux, chromatographic separation, and other technologies, the plugging components were preliminarily analyzed. The plugging components were analyzed via XRD, infrared analysis, 1H NMR, and 13C NMR. Through analysis and testing, XRD results showed that the solid compositions were clay and sand grains. Meanwhile, the infrared analysis, 1H NMR, and 13C NMR demonstrated that the main components of the plug are asphaltene crude oil, and the proportion of aromatic components and saturated components is close.