Cortical and medullary lipids of normal and nephrosclerotic human kidney

Interdependence of O₂ Consumption, Renal NEFA Pattern and N-Acetylation of PAH in the Isolated Perfused Rat Kidney

“…Unfortunately, the isolated cell-free per fused rat kidney of this type contains large anoxic areas at Q ()2~0.1 mlxg"lxm in'1 and hypoxic regions in the cortex may be present even at Q o,~0.16 mlxg^xmin"1 [6,7,13], Even if we assume that due to the HES con tent ofthe perfusate, our present preparation is somewhat superior to that reported by Franke el al. [7], 0 2 supply to tissue mito chondrial space is most probably marginal at Q 0, below 0.15 mlxg"lxmin*1 [6,7,13], This assumption is supported by the fact that the amount and pattern of N E FA in the isolated kidney at Q c>2~0.13-0.15 mlxg"1 xmin-1 do not deviate significantly from those of the rat kidney in vivo or from those of other species [4,12,20], even without tak ing into account the N EFA content of the blood trapped within the kidney in situ. Lowering the O , supply raises the overall tissue N EFA concentration by increasing, especially, the portion of fatty acids with 18 C atoms.…”

“…Our in vitro experiments indicate that N EFA of the chain length o f C l4-C 20, which make up the overwhelming part of the fatty acid content ofthe renal tissue [4,12,18,20], inhibits the enzyme arylamine-N-acetyltransferase competitively ( fig. 1).…”

Contribution of Proteinuria to Progressive Renal Injury: Consequences of Tubular Uptake of Fatty Acid Bearing Albumin

“…The functional implications of these findings are unknown, but intracellular signalling may be altered. The fate of albumin-borne fatty acids in this model is in general accord with their distribution in human kidney cortical lipids [139]. Oleate is the most prevalent fatty acid of cortical triglycerides, while palmi tate is a prominent component of cortical diglycerides and phosphatidylcholines [ 139],…”

Lipid class distribution of fatty acids including conjugated linoleic acids in healthy and cancerous parts of human kidneys

“…In the literature, contradictory results are described. Druilhet et al (20) found that cortical renal lipids were composed of 58% PL and 42% NL, but the extract of normal kidney medulla contained less PL (37.7%) and more NL (62.3%). In comparison with our data, they reported markedly higher percentages of CE, Chol., and FFA, but smaller contents of TAG, SM, PC, and PE in cortical as well as medullary renal lipids.…”

“…Several factors contributed to the large variation, including the relatively small sample size (19 patients), differences in the tumor classification of these renal patients, differences in the nutritional (dietary) status of the patients, and differences in the renal zone sampled. Several authors have shown that there are differences in the distribution of individual lipid classes in the two renal zones, i.e., the cortex and medulla (20,21), but the FA composition of kidney lipids is mainly determined by the lipid fractions, and to a much lesser extent by the zones (21). These nutritional and compositional differences were minimized by comparing the adjacent healthy and cancerous tissue from each patient independently using a t-test for paired values.…”