Corticotrophin-releasing hormone inhibits insulin-like growth factor-I release from primary cultures of rat granulosa cells

Calogero, A. E.; Barreca, Antonina; Burrello, Nunziatina; Palermo, I.; Giordano, Giulio; D’Agata, Rosario; Vicari, Enzo

doi:10.1677/joe.0.1740493

Cited by 13 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This differs from previous findings, where CRH suppressed the release of oestrogen (and insulin-like growth factor-I) from primary cultures of rat granulosa cells [17] and was able to inhibit the IL-1 mediated production of oestrogen and progesterone using granulose-theca cells from women undergoing IVF treatment [12] This study suggests a positive correlation between successful IVF outcomes and follicular fluid levels of CRH greater than 145 pg/mL. This finding is unexpected secondary to known knowledge that the hormone inhibits thecal steroid production [12] which is required for oocyte maturation.…”

Section: Discussioncontrasting

confidence: 99%

Follicular Fluid Cortisol Releasing Hormone (CRH) Levels and Assisted Reproductive Treatment (ART) Outcomes

Lim¹,

Supramaniam²,

Mittal³

et al. 2017

OJOG

View full text Add to dashboard Cite

Purpose: Can Cortisol Releasing Hormone (CRH) levels in follicular fluid predict outcomes following assisted reproductive treatment (ART) cycles?Methods: Prospective cohort study of 50 women undergoing in vitro fertilisation (IVF)/intra-cytoplasmic sperm injection (ICSI) cycles over a two month study period. All patients were treated on the long stimulation protocol; follicular fluid was aspirated and pooled for each patient. The samples were processed appropriately and assayed using CRH radioimmunoassay (RIA). Results: This study confirmed that CRH was present in follicular fluid. The average level detected was 173 ± 9 pg/mL (mean ± standard error of mean [SEM]). The data suggests a positive correlation of CRH follicular fluid levels greater than 145 pg/mL with successful ART outcomes. Conclusion: The data indicates a positive correlation between ART outcomes and the presence of follicular fluid CRH levels greater than 145 pg/mL. The results should be interpreted with caution due to the small sample size and pooling of follicular fluid per patient. Furthermore, the pooling of follicular fluid is not representative of CRH levels in an individual follicle, and thus, mature oocyte. This study serves as a reminder to what has previously been hypothesised.

show abstract

Section: Discussioncontrasting

confidence: 99%

Follicular Fluid Cortisol Releasing Hormone (CRH) Levels and Assisted Reproductive Treatment (ART) Outcomes

Lim¹,

Supramaniam²,

Mittal³

et al. 2017

OJOG

View full text Add to dashboard Cite

show abstract

“…In addition, IGF1 exerts a synergistic role with FSH on steroidogenesis of cultured rat granulosa cells [76]. Furthermore, CRH suppressed the release of both estrogen and IGF1 in cultured rat granulosa cells, and this suppressive effect was overcome by the addition of low doses of exogenous IGF1 [36]. Taken together, the data suggest that the inhibitory effect of CRH on estrogen release was mediated by a suppression of the autocrine/paracrine action of IGF1.…”

Section: Liang Et Almentioning

confidence: 75%

“…Peripheral CRH has been identified in the reproductive system, including the ovary and testis [18]. CRH suppressed estrogen release from cultured rat granulosa cells by inhibiting release of insulin-like growth factor 1 (IGF1) [35,36], and both estrogen and IGF1 inhibited apoptosis in different cells, including granulosa cells [37][38][39]. Furthermore, recent findings support the idea that CRH participates in the stress-induced apoptosis of hippocampal neurons [40].…”

Section: Introductionmentioning

confidence: 74%

See 1 more Smart Citation

Restraint Stress Impairs Oocyte Developmental Potential in Mice: Role of CRH-Induced Apoptosis of Ovarian Cells1

Liang

Wei

Cheng

et al. 2013

Biology of Reproduction

View full text Add to dashboard Cite

This study examined the role of CRH-induced ovarian cell apoptosis in the restraint stress (RS)-induced impairment of oocyte competence. Oocyte percentages of apoptotic cumulus cells (CCs) did not differ between stressed and control mice before in vitro maturation (IVM) but became significantly higher in stressed mice after IVM without serum, growth factor, and hormone. The level of Bcl2 mRNA decreased significantly in mural granulosa cells (MGCs) and ovarian homogenates after RS. Whereas ovarian estradiol, testosterone, and IGF1 decreased, cortisol and progesterone increased significantly following RS. RS increased the level of CRH in serum, ovary, and oocyte while enhancing the expression of CRHR1 in CCs, MGCs, and thecal cells. RS down-regulated ovarian expression of glucocorticoid receptor and brain-derived neurotrophic factor. Furthermore, CRH supplementation to IVM medium impaired oocyte developmental potential while increasing apoptotic CCs, an effect that was completely overcome by addition of the CRHR1 antagonist antalarmin. Results suggest that RS impaired oocyte competence by increasing CRH but not glucocorticoids. Increased CRH initiated a latent apoptotic program in CCs and oocytes during their intraovarian development, which was executed later during IVM to impair oocyte competence. Thus, elevated CRH interacted with increased CRHR1 on thecal cells and MGCs, reducing the production of testosterone, estrogen, and IGF1 while increasing the level of progesterone. The imbalance between estrogen and progesterone and the decreased availability of growth factors triggered apoptosis of MGCs and facilitated CC expression of CRHR1, which interacted with the oocyte-derived CRH later during IVM to induce CC apoptosis and reduce oocyte competence.

show abstract

“…Especially in the rat, CRH suppresses estrogen production from granulosa cells in vitro (13). On the other hand, the abundant availability of CRH-R antagonists has led to extensive research focusing on the stress axis and the diseases that may be associated with stress (14).…”

mentioning

confidence: 99%

The Effect of CRH and Its Inhibitor, Antalarmin, on in Vitro Growth of Preantral Mouse Follicles, Early Embryo Development, and Steroidogenesis

et al. 2013

View full text Add to dashboard Cite

In vitro growth systems of preantral follicles allow studying the effect of various endocrine, paracrine, and autocrine factors on follicular growth and oocyte maturation. CRH is a 41-amino-acid neuropeptide responsible for endocrine, autonomic, immunological, and behavioral responses of mammals to stress and has two receptors, CRH receptor type 1 (CRH-R1) and CRH-R2. Antalarmin, a CRH-R1 antagonist, has been used to elucidate the role of CRH in stress, inflammation, and reproduction. The present study describes in vitro growth of mouse preantral follicles, early embryo development, and steroidogenesis in the presence of CRH and its antagonist antalarmin. We cultured 732 follicles in control media, 1306 in CRH 10(-7) mol/liter, and 1202 in CRH 10(-7) plus antalarmin 10(-6) mol/liter. The culture medium was assayed on alternate days for 17β-estradiol, progesterone, and β-human chorionic gonadotropin. Total RNA was extracted from preantral follicles as well as early preimplantation embryos and was assessed by real-time RT-PCR for the expression of CRH-R1 and CRH-R2 mRNAs. Hormone analysis showed that the CRH group had lower levels of 17β-estradiol, progesterone, and β-human chorionic gonadotropin as the culture progressed, in comparison with the other two groups. RT-PCR demonstrated the presence of CRH-R1 and CRH-R2 in all stages of preantral follicle culture. Morula/blastocyst-stage embryos expressed only CRH-R1. In conclusion, CRH has an inhibitory effect on in vitro fertilized oocytes, resulting from cultured preantral follicles at all stages of preimplantation embryo development. Furthermore, the presence of CRH in the culture medium inhibits steroidogenesis by preantral mouse follicles cultured in vitro.

show abstract

Corticotrophin-releasing hormone inhibits insulin-like growth factor-I release from primary cultures of rat granulosa cells

Cited by 13 publications

References 26 publications

Follicular Fluid Cortisol Releasing Hormone (CRH) Levels and Assisted Reproductive Treatment (ART) Outcomes

Follicular Fluid Cortisol Releasing Hormone (CRH) Levels and Assisted Reproductive Treatment (ART) Outcomes

Restraint Stress Impairs Oocyte Developmental Potential in Mice: Role of CRH-Induced Apoptosis of Ovarian Cells1

The Effect of CRH and Its Inhibitor, Antalarmin, on in Vitro Growth of Preantral Mouse Follicles, Early Embryo Development, and Steroidogenesis

Contact Info

Product

Resources

About