Corylin suppresses metastasis of breast cancer cells by modulating miR-34c/LINC00963 target

Liu, Shourong; Wang, Li; Zhang, Run

doi:10.1080/19932820.2021.1883224

Cited by 12 publications

(5 citation statements)

References 16 publications

(20 reference statements)

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“… 28 Studies have indicated that miR-34c is closely related to bone metastasis of cancer. 29 Here, we found that the expression of miR-34c was inhibited after knockdown of OPN. However, the pathway through which the low expression of OPN mediates the suppression of miR-34c still needs to be further explored.…”

Section: Discussionmentioning

confidence: 77%

Deletion of osteopontin in non-small cell lung cancer cells affects bone metabolism by regulating miR-34c/Notch1 axis: a clue to bone metastasis

et al. 2023

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Lung cancer is prone to bone metastasis, and osteopontin (OPN) has an important significance in maintaining bone homeostasis. The goal of this study was to explore the impact of OPN level on bone metabolism and the molecular mechanism of inhibiting bone metastasis in non-small cell lung cancer (NSCLC). The expression of OPN in NSCLC was ascertained by Western blot and immunohistochemistry, and the correlation between the expression level of OPN and survival of patients was analyzed. Then the shRNA technology was applied to reduce the expression of OPN in NSCLC cells, and CCK-8 assay was carried out to investigate the effect of low expression of OPN on the proliferation of NSCLC cells. In addition, the effects of low expression of OPN on osteoclast differentiation, osteoblast generation and mineralization were studied using osteoclast precursor RAW264.7 and human osteoblast SaOS-2 cells, and whether OPN could regulate miR-34c/ Notch pathway to affect bone metabolism was further explored. The findings showed that the high level of OPN in NSCLC was closely related to the poor prognosis of patients and the abnormal proliferation of NSCLC cell lines. The suppression of OPN was beneficial to inhibit the differentiation of osteoclasts and promote the mineralization of osteoblasts. Besides, this study confirmed that the deletion of OPN can regulate bone metabolism through the regulation of miR-34c/Notch1 pathway, which will contribute to inhibiting the occurrence of osteolytic bone metastasis in NSCLC.

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Section: Discussionmentioning

confidence: 77%

Deletion of osteopontin in non-small cell lung cancer cells affects bone metabolism by regulating miR-34c/Notch1 axis: a clue to bone metastasis

et al. 2023

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“…The cell migration assays were conducted by referring to previous studies, and the specific operation was as follows [ 22 , 23 ]. The upper chamber of the 24-well transwell plate (Corning, USA) was precoated with Matrigel (BD Biosciences, USA) for invasion assays, and inserts without Matrigel were used for migration assays.…”

Section: Methodsmentioning

confidence: 99%

Prognostic values of regulatory T cells (Tregs) and Treg-related genes in gastric cancer

Zheng¹,

Li²,

Song³

et al. 2023

cejoi

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Introduction:This study attempted to investigate the potential of a risk model constructed for regulatory T cells (Tregs) and their related genes in predicting gastric cancer (GC) prognosis.Material and methods: We used flow cytometry to detect the content of CD4 + CD25 + Tregs. After detecting expression of five Treg-related genes by quantitative real-time polymerase chain reaction (qRT-PCR), Pearson analysis was employed to analyze the correlation between Tregs and related gene expression. 3-( 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation and transwell assays were used to detect the effects of a disintegrin and metalloproteinase with thrombospondin motifs 12 (ADAMTS12) on cell functions. A prognostic risk model was built after Cox regression analysis. The Kaplan-Meier method was employed to assess how Tregs, 5-gene risk scores and expression of 5 genes were correlated with the survival time.Results: A significantly increased content of Tregs was found in GC tissues (p < 0.05). 5 Tregrelated genes were significantly up-regulated in GC with a positive correlation with the content of Tregs (p < 0.05). Overexpression of ADAMTS12 significantly enhanced the viability, proliferation, migration and invasion of tumor cells. Kaplan-Meier analysis demonstrated poor overall survival and disease-free survival in the high-risk group. The results of survival analysis of Treg content and related gene expression were consistent with those of Cox analysis.Conclusions: The risk model constructed based on five Treg-related genes can enable effective prediction in the prognosis of GC patients.

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“…Pec inhibited migration and invasion of MDA-MB-231, MCF-7 and 4T1 cells in vitro through downregulation of p-stat3, MMP-2 and MMP-9 hence inhibiting stat3 signalling pathway (Li et al, 2019). Corylin reduced migration, invasion and EMT of MCF-7 and MDA-MB-231 cells through miR-34c/LIN00963 target (Liu et al, 2021).…”

Section: Inhibition Of Migration and Invasion (Anti-metastasis)mentioning

confidence: 97%

Molecular mechanisms of flavonoids and their modulatory effects against breast cancer: A scoping review

Murithi

Wambugu²,

Nyanjom

et al. 2022

F1000Res

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Background: Breast cancer is the most prevalent malignancy among women. It is a disease whose incidence and mortality rates are on the upsurge globally. Debilitating effects, cost and resistance to available chemotherapeutic interventions render them unideal. Dietary phytochemicals have been shown to have preventive and therapeutic effects. Research continues to affirm the role of flavonoids as potential chemotherapeutic agents in combating the disease. Understanding modulation of key cellular signalling pathways by flavonoids presents promising molecular targets that may be leveraged to develop better chemotherapeutic agents for breast cancer. Methods: To describe the in vitro and in vivo modulatory effects of flavonoids on molecular anti-cancer mechanisms we searched three databases. We included original articles describing modulation of cell signalling processes such as; cell cycle, apoptosis, autophagy, angiogenesis, invasion and migration which are involved in tumorigenesis. The search guidelines such as; year of publication, search strategy, study design and language informed article selection. Results: Thirty-six articles were reviewed. Modulatory effects of six subclasses of flavonoids on breast cancer tumorigenic pathways were reported. The effects included enhanced apoptosis, attenuation of; angiogenesis, cell cycle, invasion, migration and metastasis. For instance, pectolinarigenin inhibited signal transducer and activator of transcription 3 (stat3) signalling pathway in triple negative breast cancer. Whereas, sideritoflavone caused cell cycle arrest and inhibited migration in trastuzumab resistant breast cancer cells. Additionally, quercetin and ampelopsin inhibited stemness features in triple negative breast cancer cells. Conclusion: Evidently flavonoids showed significant modulatory effects on cellular signalling pathways crucial for breast cancer progression. The ability of flavonoids to act on a wide range of mechanisms as well as on aggressive breast cancer types presents an array of hope. We recommend that further studies be done to ascertain the applicability of these compounds in treatment of breast cancers.

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Corylin suppresses metastasis of breast cancer cells by modulating miR-34c/LINC00963 target

Cited by 12 publications

References 16 publications

Deletion of osteopontin in non-small cell lung cancer cells affects bone metabolism by regulating miR-34c/Notch1 axis: a clue to bone metastasis

Deletion of osteopontin in non-small cell lung cancer cells affects bone metabolism by regulating miR-34c/Notch1 axis: a clue to bone metastasis

Prognostic values of regulatory T cells (Tregs) and Treg-related genes in gastric cancer

Molecular mechanisms of flavonoids and their modulatory effects against breast cancer: A scoping review

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