Corynebacterium pyruviciproducens sp. nov., a pyruvic acid producer

Abstract

A coryneform strain, 06-1773O T (5WAL 19168 T ), derived from a groin abscess sample was characterized using phenotypic and molecular taxonomic methods. Comparative analyses revealed more than 3 % divergence of the 16S rRNA gene sequence and about 10 % divergence of the partial rpoB gene sequence from the type strain of Corynebacterium glucuronolyticum. The strain could also be differentiated from C. glucuronolyticum by a set of phenotypic properties. A DNA-DNA relatedness study between strain WAL 19168 T and C. glucuronolyticum CCUG 35055 T showed a relatedness value of 13.3 % (13.7 % on repeat analysis). The genotypic and phenotypic data show that the strain merits classification within a novel species of Corynebacterium. We propose the name Corynebacterium pyruviciproducens sp. nov. for the novel species. The type strain is 06-1773O T (5WAL 19168 T 5CCUG 57046 T 5ATCC BAA-1742 T ).Bacteria of the genus Corynebacterium have been recovered extensively from mammals and the environment (Feurer et al., 2004; Hommez et al., 1999). Except for Corynebacterium diphtheriae, the aetiological agent of diphtheria, most species of the genus are regarded as opportunistic pathogens (Paviour et al., 2002). Compared with those bacteria possessing proof of clinical pathogenicity, less emphasis has been put on Corynebacterium. Recently, however, a report demonstrated that Corynebacterium was the most prevalent bacterial genus associated with chronic wound infections in diabetics (possibly skin flora) (Dowd et al., 2008). In our investigation of the bacteriology of acute wound infections (Finegold et al., 2008), a novel aerobic, rod-shaped, Gram-positive corynebacterium was found. It is similar to Corynebacterium glucuronolyticum (Devriese et al., 2000) by phenotypic and genotypic identification. The aim of this polyphasic study was to describe the isolation and characterization of this novel organism.A groin abscess specimen was collected at Olive View-UCLA Medical Center in 2006, and studied in the Wadsworth Anaerobic Laboratory (WAL). One isolate derived from this specimen, WAL 19168 T , was analysed by phenotypic and genotypic characterization methods.Optimal growth conditions were investigated by cultivating strain WAL 19168 T aerobically and anaerobically on trypticase soy agar with 5 % sheep blood (TSA-II; Becton Dickinson Microbiology Systems) at 20, 37 and 42 u C. Lipid requirement was determined by comparison between the culture in simple brain heart infusion (BHI) broth (MP Biomedicals) and in the same medium supplemented with 1 % Tween 80 after 72 h at 37 u C (Riegel et al., 1994). Further confirmation of lipid stimulation of growth was observed by the 'response to serum' test (Coyle & Lipsky, 1990). For cellular fatty acid (CFA) and metabolic end product (ME) (short-chain volatile and non-volatile fatty acids) analyses, the strains were grown in BHI broth supplemented with 1 % glucose and 5 % rabbit serum (MP Biomedicals). CFAs of the strains were detected with a Hewlett Packard 5890 series II gas chromatograph, wh...

Recognition of Potentially Novel Human Disease-Associated Pathogens by Implementation of Systematic 16S rRNA Gene Sequencing in the Diagnostic Laboratory

“…For example, within the Corynebacterium genus, 18 novel species were validly described from 2004 to 2009. A total of 5 of these, namely, Corynebacterium freiburgense (12), Corynebacterium pyruviciproducens (26), C. mastitidis (10,18), C. massiliense (18), and C. ureicelerivorans (11,31), were also identified in our study. When we calculated phylogenetic trees based on partial 16S rRNA sequences (Fig.…”

“…Of those, 60 isolates (0.4‰; see Table S1 in the supplemental material) had a 16S rRNA gene homology of Ͻ99% to members of accepted taxa on the date of the first interpretation. A total of 11 of the 60 sequences with a 16S rRNA homology of Ͻ99% in the first-time analysis could be allocated to a species established during the study term as a novel species by others: Acinetobacter septicus (16,20), Brevibacterium ravenspurgense (17), Corynebacterium freiburgense (12), Corynebacterium massiliense (n ϭ 2) (18), C. mastitidis (10,18), C. pyruviciproducens (26), C. ureicelerivorans (11,31), Neisseria zoodegmatis (28), Paenibacillus barengoltzii (21), and the reclassified Campy- lobacter ureolyticus (previously known as Bacteroides ureolyticus) (27). We calculated dendrograms to assess phylogenetic relationships (Fig.…”

Extended Characterization of Corynebacterium pyruviciproducens Based on Clinical Strains from Canada and Switzerland

“…T (1). These observations as well as heterogeneities in the 16S rRNA sequences are presented in this report.…”

“…These observations as well as heterogeneities in the 16S rRNA sequences are presented in this report. In the species description publication, Tong and colleagues (1) reported that the C. pyruviciproducens type strain was highly sensitive to ampicillin, ceftriaxone, clindamycin, and erythromycin. Since no other information concerning the antimicrobial susceptibility of C. pyruviciproducens has been published to date, we present in this report detailed data on the antimicrobial susceptibility testing of isolates from our laboratories, including new susceptibility data for the strain 06-17730 T using an extended panel of antimicrobials.…”

“…T ϭ ATCC BAA-1742 T ϭ DSM 45585 T ) cultured from a groin abscess (1). This bacterial species has now been isolated from several clinical specimens in laboratories in Switzerland and Canada.…”