Coselection for resistance to multiple late-generation human therapeutic antibiotics encoded on tetracycline resistance plasmids captured from uncultivated stream and soil bacteria

Herrick, James B.; Haynes, Richard K.; Heringa, Spencer; Brooks, J M; Sobota, L T

doi:10.1111/jam.12538

Cited by 19 publications

(14 citation statements)

References 45 publications

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“…In any case, the oral use of low bioavailability antibiotics in intensive animal production that do not get over MIC 90, represents a crucial risk factor for antimicrobial resistance development and therapeutic failures. Microbiota in caecum and colon is exposed to high concentrations of unabsorbed fraction of the OTC administered (>90%) (Hansen, Aarestrup, & Sørensen, 2002;Toutain, Ferran, Bousquet-Melou, Pelligand, & Lees, 2016), enhancing multidrug-resistant strains (Herrick, Haynes, Heringa, Brooks, & Sobota, 2014;Toutain et al, 2016) and drug dissemination in the environment (Cheng et al, 2014). Accounting for the seriousness of this situation and in a context where rational use of antibiotics is essential, we think that if low oral bioavailability has been demonstrated for a certain formulation, as it is the case of OTC in the present study, its oral use should not be acceptable to treat systemic infections.…”

Section: Otc Bioavailability Was Low In All Treatment Groups With Highmentioning

confidence: 99%

Impact of water hardness on oxytetracycline oral bioavailability in fed and fasted piglets

Decundo

Dieguez

Martínez

et al. 2019

Veterinary Medicine & Sci

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Water hardness is a critical factor that affects oxytetracycline dissolution by chelation with cations. These interactions may lead to impaired dosing and consequently decrease absorption. Moreover, feed present in gastrointestinal tract may interact with antibiotic and alter pharmacokinetic parameters. In the present study, dissolution profiles of an oxytetracycline veterinary formulation were assessed in purified, soft and hard water. Furthermore, oxytetracycline absolute bioavailability, after oral administration of the drug dissolved in soft or hard water, was evaluated in fed and fasted piglets. A maximum dissolution of 86% and 80% was obtained in soft and hard water, respectively, while in purified water dissolution was complete. Results from in vivo study reconfirmed oxytetracycline's very low oral bioavailability. The greatest values were attained when antibiotic was dissolved in soft water and in fasted animals. Statistically significant lower absolute bioavailability was achieved when hard water was used and/or animals were fed. Moreover, Cmax attained in all treatments was lower than MIC90 of most important swine pathogens. For these reasons, the oral use of OTC formulations, that have demonstrated low oral bioavailability, should be avoided to treat systemic diseases in pigs.

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Section: Otc Bioavailability Was Low In All Treatment Groups With Highmentioning

confidence: 99%

Impact of water hardness on oxytetracycline oral bioavailability in fed and fasted piglets

Decundo

Dieguez

Martínez

et al. 2019

Veterinary Medicine & Sci

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show abstract

“…Furthermore, these efflux pump genes and ARGs are highly homologous and functionally similar. Through the integration of intI1, OPs tolerance genes and ARGs also can be inserted into one or more of the same MGEs simultaneously (Martinaz et al, 2009;Herrick et al, 2014). Therefore, when the bacterial OPs tolerance genes were promoted, the expression of relevant ARGs was activated at the same time, thus resulting indirectly in fluctuations of soil ARG abundance influenced by bioaccessible OPs.…”

Section: Correlation Between Inti1 Agr Abundance and Pollutant Concmentioning

confidence: 99%

Positive relationship detected between soil bioaccessible organic pollutants and antibiotic resistance genes at dairy farms in Nanjing, Eastern China

Sun

et al. 2015

Environmental Pollution

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“…This approach enables characterization of all AMR genes within a microbial community (the “resistome”), which can be useful in understanding evolutionary shifts in AMR [ 1 ], as well as for detecting transfer of diverse AMR genes between hosts, environments, or uncultivable organisms [ 2 ]. AMR is an inherently ecological phenomenon, with processes including transfer of genetic elements between divergent bacteria, increased promiscuity and mutation in the face of bacterial stress and inflammation, and co-selection and co-mobility of multiple genes [ 3 – 6 ]. Shotgun metagenomics represents a tool for advancing our understanding of these interactions by enabling access to the genetic material of the microbial population as a whole [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Enrichment allows identification of diverse, rare elements in metagenomic resistome-virulome sequencing

Noyes¹,

Weinroth²,

Parker³

et al. 2017

Microbiome

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BackgroundShotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to limitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias.ResultsThe use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abundance portion of the resistome-virulome that was more diverse and compositionally different than that detected by more traditional metagenomic assays. The low-abundance portion of the resistome-virulome also contained resistance genes with public health importance, such as extended-spectrum betalactamases, that were not detected using traditional shotgun metagenomic sequencing. In addition, the use of the bait-capture and enrichment system enabled identification of rare resistance gene haplotypes that were used to discriminate between sample origins.ConclusionsThese results demonstrate that the rare resistome-virulome contains valuable and unique information that can be utilized for both surveillance and population genetic investigations of resistance. Access to the rare resistome-virulome using the bait-capture and enrichment system validated in this study can greatly advance our understanding of microbiome-resistome dynamics.Electronic supplementary materialThe online version of this article (10.1186/s40168-017-0361-8) contains supplementary material, which is available to authorized users.

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Coselection for resistance to multiple late-generation human therapeutic antibiotics encoded on tetracycline resistance plasmids captured from uncultivated stream and soil bacteria

Cited by 19 publications

References 45 publications

Impact of water hardness on oxytetracycline oral bioavailability in fed and fasted piglets

Impact of water hardness on oxytetracycline oral bioavailability in fed and fasted piglets

Positive relationship detected between soil bioaccessible organic pollutants and antibiotic resistance genes at dairy farms in Nanjing, Eastern China

Enrichment allows identification of diverse, rare elements in metagenomic resistome-virulome sequencing

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