Cosmetic Potential of Enzymatic Treated Ginseng Leaf

Lee, Hyun Sun; Lee, Hyun Jung; Cho, Hye Jin; Park, Sung Sun; Kim, Jin Man; Suh, Hyung Joo

doi:10.5142/jgr.2010.34.3.227

Cited by 10 publications

(7 citation statements)

References 35 publications

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“…The ginsenoside content of UTGL was higher than that of the control [26] . Our previous study [19] confirmed that UTGL is significantly higher in total phenolic compounds that are responsible for the antioxidant activities and inhibitory activity on elastase, compared to nontreated ginseng leaf.…”

Section: Discussionsupporting

confidence: 67%

“…Unfortunately, these compounds are strongly linked with cellulose, pectin, or β-glucan. In our previous work [19] , we found that Ultraflo L treated ginseng leaf (UTGL) improved ginsenoside recovery through liberation of polyphenols and flavonoids. Polyphenols are regarded as secondary metabolites that are synthesized in plants and function as a defense mechanism [20] .…”

Section: Introductionmentioning

confidence: 96%

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Photoprotective effects of topical ginseng leaf extract using Ultraflo L against UVB-induced skin damage in hairless mice

Hong

Lee²,

Jung

et al. 2017

Journal of Ginseng Research

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BackgroundAbnormal activation of matrix metalloproteinases (MMPs) plays an important role in UV-induced wrinkle formation, which is a major dermatological problem. This formation occurs due to the degeneration of the extracellular matrix (ECM). In this study, we investigated the cutaneous photoprotective effects of Ultraflo L treated ginseng leaf (UTGL) in hairless mice.MethodsSKH-1 hairless mice (6 weeks of age) were randomly divided into four groups (8 mice/group). UTGL formulation was applied topically to the skin of the mice for 10 weeks. The normal control group received nonvehicle and was not irradiated with UVB. The UV control (UVB) group received nonvehicle and was exposed to gradient-UVB irradiation. The groups (GA) receiving topical application of UTGL formulation were subjected to gradient-UVB irradiation on 0.5 mg/cm2 [GA-low (GA-L)] and 1.0 mg/cm2 [(GA-high (GA-H)] of dorsal skin area, respectively.ResultsWe found that topical treatment with UTGL attenuated UVB-induced epidermal thickness and impairment of skin barrier function. Additionally, UTGL suppressed the expression of MMP-2, -3, and -13 induced by UVB irradiation. Our results show that topical application of UTGL protects the skin against UVB-induced damage in hairless mice and suggest that UTGL can act as a potential agent for preventing and/or treating UVB-induced photoaging.ConclusionUTGL possesses sunscreen properties and may exhibit photochemoprotective activities inside the skin of mice. Therefore, UTGL could be used as a potential therapeutic agent to protect the skin against UVB-induced photoaging.

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Section: Discussionsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 96%

Photoprotective effects of topical ginseng leaf extract using Ultraflo L against UVB-induced skin damage in hairless mice

Hong

Lee²,

Jung

et al. 2017

Journal of Ginseng Research

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“…Ginsenosides are a special group of triterpenoid saponins in Panax ginseng , which exhibit potential anticarcinogenic, immunomodulatory, anti‐fatigue, and antiallergic activities (Christensen, ). They are usually used in pharmaceutical and health food preparations (Lee et al, ). Ginsenosides are extracted from ginseng or its hairy root, which is time consuming and labor‐intensive.…”

Section: Introductionmentioning

confidence: 99%

Optimization of a cytochrome P450 oxidation system for enhancing protopanaxadiol production in Saccharomyces cerevisiae

Zhao

Bai

Liu

et al. 2016

Biotech & Bioengineering

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Ginsenosides, the major bioactive components of Panax ginseng, are regarded as promising high-value pharmaceutical compounds. In ginseng, ginsenosides are produced from their precursor protopanaxadiol. Recently, an artificial biosynthetic pathway of protopanaxadiol was built in Saccharomyces cerevisiae by introducing a P. ginseng dammarenediol-II synthase, a P. ginseng cytochrome P450-type protopanaxadiol synthase (PPDS), and a Arabidopsis thaliana NADPH-cytochrome P450 reductase (ATR1). In this engineered yeast strain, however, the low metabolic flux through PPDS resulted in a low productivity of protopanaxadiol. Moreover, health of the yeast cells was significantly affected by reactive oxygen species released by the pool coupling between PPDS and ATR1. To overcome the obstacles in protopanaxadiol production, PPDS was modified through transmembrane domain truncation and self-sufficient PPDS-ATR1 fusion construction in this study. The fusion enzymes conferred approximately 4.5-fold increase in catalytic activity, and 71.1% increase in protopanaxadiol production compared with PPDS and ATR1 co-expression. Our in vivo experiment indicated that the engineered yeast carrying fusion protein effectively converted 96.8% of dammarenediol-II into protopanaxadiol. Protopanaxadiol production in a 5 L bioreactor in fed-batch fermentation reached 1436.6 mg/L. Our study not only improved protopanaxadiol production in yeast, but also provided a generic method to improve activities of plant cytochrome P450 monooxygenases. This method is promising to be applied to other P450 systems in yeast. Biotechnol. Bioeng. 2016;113: 1787-1795. © 2016 Wiley Periodicals, Inc.

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“…The solid‐phase extraction sample (2 mL) was prepared using the C18 ODS cartridge (Waters Associates, Milford, MA, USA) as described by Lou et al . The levels of 14 major ginsenosides were analyzed using a high‐performance liquid chromatography (HPLC)‐based technique described by Lee et al . The HPLC system (Varian Prostar 200, Reno, NV, USA) was equipped with a quaternary solvent delivery system, an autosampler, and a UV detector.…”

Section: Methodsmentioning

confidence: 99%

Enzymatic hydrolysis increases ginsenoside content in Korean red ginseng (Panax ginseng CA Meyer) and its biotransformation under hydrostatic pressure

et al. 2019

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BACKGROUND Enzymatic hydrolysis and high hydrostatic pressure (HHP) are common processing techniques in the extraction of active compounds from food materials. The aim of this study was to investigate the effects of enzymatic hydrolysis combined with HHP treatments on ginsenoside metabolites in red ginseng. RESULTS The yield and changes in the levels of polyphenol and ginsenoside were measured in red ginseng treated with commercial enzymes such as Ultraflo L, Viscozyme, Cytolase PCL5, Rapidase and Econase E at atmospheric pressure (0.1 MPa), 50 MPa, and 100 MPa. β‐Glucosidase activity of Cytolase was the highest at 4258.2 mg−1, whereas Viscozyme showed the lowest activity at 10.6 mg−1. Pressure of 100 MPa did not affect the stability or the activity of the β‐glucosidase. Treatment of red ginseng with Cytolase and Econase at 100 MPa significantly increased the dry weight and polyphenol content of red ginseng, compared with treatments at 0.1 MPa and 50 MPa (P < 0.05). The amounts of ginsenoside and ginsenoside metabolites derived from red ginseng processed using Cytolase were higher than those derived from red ginseng treated with the other enzymes. Treatment with Cytolase also significantly increased the skin and intestinal permeability of red ginseng‐derived polyphenols. CONCLUSION Cytolase could be useful as an enzymatic treatment to enhance the yield of bioactive compounds from ginseng under HHP. In addition, ginsenoside metabolites obtained by Cytolase hydrolysis combined with HHP are functional substances with increased intestinal and skin permeability. © 2019 Society of Chemical Industry

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Cosmetic Potential of Enzymatic Treated Ginseng Leaf

Cited by 10 publications

References 35 publications

Photoprotective effects of topical ginseng leaf extract using Ultraflo L against UVB-induced skin damage in hairless mice

Photoprotective effects of topical ginseng leaf extract using Ultraflo L against UVB-induced skin damage in hairless mice

Optimization of a cytochrome P450 oxidation system for enhancing protopanaxadiol production in Saccharomyces cerevisiae

Enzymatic hydrolysis increases ginsenoside content in Korean red ginseng (Panax ginseng CA Meyer) and its biotransformation under hydrostatic pressure

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