Cotesia plutellae Bracovirus Genome and Its Function in Altering Insect Physiology

Kim, Yong Gyun; Choi, Jae Young; Je, Yeon Ho

doi:10.1016/s1226-8615(08)60351-9

Cited by 50 publications

(22 citation statements)

References 99 publications

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“…A viral histone H4 (CpBV-H4) has been identified from a polydnavirus (PDV) called Cotesia plutellae bracovirus (CpBV) (4)(5)(6). PDVs are symbiotic to some hymenopteran insect species and help a successful parasitization by host wasps of their target lepidopteran insect hosts (7).…”

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confidence: 99%

“…An endoparasitoid wasp, Cotesia plutellae, parasitizes young larvae of the diamondback moth, Plutella xylostella, and alters the host immune and developmental program with the help of parasitic factors, such as teratocytes, ovarian proteins, venom proteins, and CpBV (6). A full genome sequence of an episomal form of CpBV suggests 157 candidate genes, including CpBV-H4 (17).…”

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confidence: 99%

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A Viral Histone H4 Joins to Eukaryotic Nucleosomes and Alters Host Gene Expression

Hepat

Song

Lee

et al. 2013

J Virol

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c A viral histone H4 (CpBV-H4) is encoded in a polydnavirus, Cotesia plutellae bracovirus. Its predicted amino acid sequence is highly homologous to that of host insect histone H4 except for an extended N-terminal tail containing 38 amino acids with nine lysine residues. Its expression induces an immunosuppression of target insects by suppressing immune-associated genes, presumably through an epigenetic control. This study analyzed its molecular interaction with eukaryotic host nucleosomes and subsequent regulation of host gene expression. Purified recombinant CpBV-H4 could associate with nucleosomal components (H2A, H2B, H3, and H4) and form an octamer. Transient expression of CpBV-H4 in an insect, Tribolium castaneum, was performed by microinjection of a recombinant expression vector and confirmed by both reverse transcriptase PCR (RT-PCR) and immunoblotting assays. Under this transient expression condition, total RNAs were extracted and read by a deep-sequencing technique. Annotated transcripts were classified into different gene ontology (GO) categories and compared with those of control insects injected with a truncated CpBV-H4. Target genes manipulated by CpBV-H4 expression showing significant differences (fold changes > 10 9 ) included all GO categories, including development and immune-associated genes. When the target genes were physically mapped, they were found to be scattered on entire chromosomes of T. castaneum. In addition, chromatin immunoprecipitation against CpBV-H4 determined 16 nucleosome sites (P < 10 ؊5 ) of the viral histone incorporation, which were noncoding regions near DNA-binding and inducible genes. These findings suggest that the viral histone H4 alters host gene expression by a direct molecular interaction with insect nucleosomes.

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confidence: 99%

mentioning

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A Viral Histone H4 Joins to Eukaryotic Nucleosomes and Alters Host Gene Expression

Hepat

Song

Lee

et al. 2013

J Virol

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“…Its symbiotic PDV is the C. plutellae bracovirus (CpBV). Consisting of at least 27 segments, the genome of CpBV is estimated to be at least 470 kb (7). Protein tyrosine phosphatases (PTPs) are present in the largest CpBV gene family, which consists of at least 39 putative genes (8).…”

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confidence: 99%

A SERI technique reveals an immunosuppressive activity of a serine-rich protein encoded in Cotesia plutellae bracovirus

Barandoc

Park²,

Kim³

2010

BMB Reports

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“…이 때 기주 혈강에 옮겨진 PDV 입자들은 기주 세포에 침입하 고, 기주 유전자 발현 시스템을 이용하여 바이러스 자신의 유전자들을 발현시키게 된다. PDV 유래 다양한 유전자 산 물은 기주의 면역을 억제하고, 발육을 지연시켜 숙주 기생 봉의 발육을 도모하게 된다 (Kim et al, 2007).…”

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Mass Production of a Recombinant Baculovirus Expressing CpBV-ELP1 and Control of the Beet Armyworm, Spodoptera exigua

Park

Kim²

2015

The Korean Journal of Pesticide Science

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Cotesia plutellae bracovirus (CpBV) is a polydnavirus symbiotic to C. plutellae parasitizing young larvae of the diamondback moth, Plutella xylostella. Several CpBV genes play important roles in suppressing immune responses of the parasitized larvae. This study tested a hypothesis that the CpBV genes inducing host immunosuppression could be applied to develop a potent recombinant baculovirus. Based on a previous study, a recombinant baculovirus expressing CpBV-ELP1 (AcMNPV-ELP1) was selected and multiplied using larvae of the beet armyworm, Spodoptera exigua. The recombinant viruses were produced in a yield of 5 × 10 10 polyhedral inclusion body (PIB)/larva. The cultured AcMNPV-ELP1 exhibited a much higher pathogenicity against S. exigua larvae. However, its insecticidal activity was varied among larval instars of S. exigua, in which first and late instars were high susceptible. Spray of the recombinant baculovirus (5 × 10 6 PIB/mL) exhibited higher control efficacy ( 88%) against S. exigua larvae infesting cabbage than a chemical insecticide, tebufenozide, at 7 days after treatment. These results indicate that AcMNPV-ELP1 mass-cultured using host insect system is highly pathogenic and can be applied to develop a novel microbial control agent. Key words

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Cotesia plutellae Bracovirus Genome and Its Function in Altering Insect Physiology

Cited by 50 publications

References 99 publications

A Viral Histone H4 Joins to Eukaryotic Nucleosomes and Alters Host Gene Expression

A Viral Histone H4 Joins to Eukaryotic Nucleosomes and Alters Host Gene Expression

A SERI technique reveals an immunosuppressive activity of a serine-rich protein encoded in Cotesia plutellae bracovirus

Mass Production of a Recombinant Baculovirus Expressing CpBV-ELP1 and Control of the Beet Armyworm, Spodoptera exigua

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