Cotton Fiber Biotechnology: Potential Controls and Transgenic Improvement of Elongation and Cell Wall Thickening

Stiff, Michael R.; Tuttle, John R.; Graham, Benjamin; Haigler, Candace H.

doi:10.1007/978-3-319-44570-0_8

Cited by 10 publications

(13 citation statements)

References 108 publications

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“…Giving an insight into previously reported specificity of GhSCFP [15,34,35], the expression pattern of CpEXPA1 under GhSCFP promoter was found to be higher in Cotton boll than that of the CaMV35S. The GhSCFP, isolated from Cotton, when allowed to express in Arabidopsis,…”

Section: Plos Onementioning

confidence: 80%

Comparative analysis of Constitutive and fiber-specific promoters under the expression pattern of Expansin gene in transgenic Cotton

et al. 2020

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Promoters are specified segments of DNA that lead to the initiation of transcription of a specific gene. The designing of a gene cassette for plant transformation is significantly dependent upon the specificity of a promoter. Constitutive Cauliflower mosaic virus promoter, CaMV35S, due to its developmental role, is the most commonly used promoter in plant transformation. While Gossypium hirsutum (Gh) being fiber-specific promoter (GhSCFP) specifically activates transcription in seed coat and fiber associated genes. The Expansin genes are renowned for their versatile roles in plant growth. The overexpression of Expansin genes has been reported to enhance fiber length and fineness. Thus, in this study, a local Cotton variety was transformed with Expansin (CpEXPA1) gene, in the form of two separate cassettes, each with a different promoter, named as 35SEXPA1 and FSEXPA1 expressed under CaMV35S and GhSCFP promoters respectively. Integration and Spatiotemporal relative expression of the transgene were studied in an advanced generation. GhSCFP bearing transgene expression was significantly higher in Cotton fiber than other plant parts. While transgene with CaMV35S promoter was found to be continually expressing in all tissues but the expression was lower in fiber than that expressed under GhSCFP. The temporal expression profile was quite interesting with a gradual increasing pattern of both constructs from 1DPA (days post anthesis) to 18DPA and decreased expression from 24 to 30 DPA. Besides the relative expression of promoters, fiber cellulose quantification and fluorescence intensity were also observed. The study significantly compared the two most commonly used promoters and it is deduced from the results that the GhSCFP promoter could be used more efficiently in fiber when compared with CaMV35S which being constitutive in nature preferred for expression in all parts of the plant.

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Section: Plos Onementioning

confidence: 80%

Comparative analysis of Constitutive and fiber-specific promoters under the expression pattern of Expansin gene in transgenic Cotton

et al. 2020

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“…Initially, during 0-6 days post-anthesis (DPA), the cell wall of the seed epidermis protrudes out and starts extending. This extension/loosening of the cell wall attains a maximum length up to ∼2.6 to 3.6 cm length during 6-20 DPA (Stiff et al, 2016), which later entered an overlapping transition phase, in which loosen elongated cell wall finally stretched/compressed to stabilize the mature cell wall (18-22 DPA).…”

Section: Introductionmentioning

confidence: 99%

“…Initially, during 0–6 days post‐anthesis (DPA), the cell wall of the seed epidermis protrudes out and starts extending. This extension/loosening of the cell wall attains a maximum length up to ∼2.6 to 3.6 cm length during 6–20 DPA (Stiff et al., 2016), which later entered an overlapping transition phase, in which loosen elongated cell wall finally stretched/compressed to stabilize the mature cell wall (18–22 DPA). The development process finally enters the “deposition” phase of the secondary cell wall, which is a unique attribute of cotton fibre, because pure cellulose fibrils are deposited among the cell wall polymers to make it compact and mature (Rathgeber et al., 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, cotton fibre constitutes 94%–95% cellulose at the time of harvesting. The cellulose content further raises to 99% in industry through chemical processing (Coleman et al., 2009; Stein & Granot, 2019; Stiff et al., 2016). The quality of cotton fibre is highly influenced by the expression of genes involved in cell wall loosening.…”

Section: Introductionmentioning

confidence: 99%

“…(n = 3) [Colour figure can be viewed at wileyonlinelibrary.com] focused extensively. Considering the importance of previously reported promoter GhSCFP for fibre-specificity(Bai et al, 2014; Lianteng et al, 2011;Stiff et al, 2016), the expression pattern and effect of α-EXPA1 under the GhSCFP promoter was analysed in the present study. Expansin promotes cell wall loosening during 6-20 DPA(Bai et al, 2014).…”

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confidence: 99%

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Transformation of α‐EXPA1 gene leads to an improved fibre quality in Gossypium hirsutum

et al. 2020

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The Expansin protein is known for its multifaceted roles in plant growth, especially cell walls. However, very few studies have been done so far to assess the effects of expansin genes on Cotton fibre development. The present study is a successful effort to fill this gap, where the α-EXPA1 gene transformed into a local cotton variety, Gossypium hirsutum, through Agrobacterium-mediated transformation under Gossypium hirsutum Seed coat and Fibre-specific promoter (GhSCFP). The transgenic cotton plants underwent molecular characterization and fibre trait evaluation. Our results indicated that α-EXPA1 showed an up-regulated expression during the transition phase of secondary cell wall synthesis and resulted in improving the fibre parameters, especially micronaire value. Transgenic cotton fibre also showed a finer twisting under the Scanning electron microscope (SEM) as compared to non-transgenic cotton fibre samples. The fibre production is influenced by more than nine thousand genes, and the fibre improvement cannot be just achieved through a single gene transformation. However, α-EXPA1 is one of the potential candidates for cotton fibre research as it significantly improved the cotton fibre.

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Cotton Fiber Biosynthesis

Kim

2018

Cotton Fiber: Physics, Chemistry and Biology

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Cotton Fiber Biotechnology: Potential Controls and Transgenic Improvement of Elongation and Cell Wall Thickening

Cited by 10 publications

References 108 publications

Comparative analysis of Constitutive and fiber-specific promoters under the expression pattern of Expansin gene in transgenic Cotton

Comparative analysis of Constitutive and fiber-specific promoters under the expression pattern of Expansin gene in transgenic Cotton

Transformation of α‐EXPA1 gene leads to an improved fibre quality in Gossypium hirsutum

Cotton Fiber Biosynthesis

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