Counteraction of retinoic acid and 1,25-dihydroxyvitamin D3 on up-regulation of adipocyte differentiation with PPARγ ligand, an antidiabetic thiazolidinedione, in 3T3-L1 cells

Hida, Yoshifumi; Kawada, Teruo; Kayahashi, Shun; Ishihara, Tomomi; Fushiki, Tohru

doi:10.1016/s0024-3205(98)00059-9

Cited by 66 publications

(55 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In standard diet-fed animals, reduction of adiposity and body weight after tRA treatment correlated with a general down-regulation of the expression of PPAR␥2 in all the adipose depots examined. This is in agreement with previous results obtained in in vitro systems (3T3-L1 cells and brown adipocytes differentiated in primary culture), showing RA-induced down-regulation of PPAR␥ expression (21,35), and is consistent with the proposed mechanism of action of RA on preadipose cells. Thus, through binding to RAR, RA is believed to block the C/EBP␤-mediated induction of downstream genes, notably PPAR␥, at early stages of the adipogenic process (22).…”

Section: Effects Of Tra Treatmentsupporting

confidence: 93%

Changes of Adiposity in Response to Vitamin A Status Correlate with Changes of PPARγ2 Expression

et al. 2001

View full text Add to dashboard Cite

RIBOT, JOAN, FRANCISCO FELIPE, M. LUISA BONET, AND ANDREU PALOU. Changes of adiposity in response to vitamin A status correlate with changes of PPAR␥2 expression. Obes Res. 2001;9:500 -509. Objective: To gain insight into the in vivo modulation of the expression of the adipogenic transcription factors PPAR␥2, C/EBP␣, and ADD1/SREBP1c by retinoids and its relationship with whole-body adiposity. Research Methods and Procedures: Three-week-old mice were fed with standard chow or a vitamin A-deficient diet for 10 weeks. During the 4 days immediately before they were killed, the animals were treated either with all-trans retinoic acid (tRA; 100 mg/kg per day, subcutaneously) or vehicle. The specific levels of the mRNAs for the three transcription factors were analyzed in epididymal white adipose tissue (eWAT) and inguinal white adipose tissue and in brown adipose tissue (BAT). Other parameters determined were leptin and UCP2 levels in white adipose tissue depots, total cholesterol and triglyceride serum levels, energy intake, body weight, and adiposity. Results: Vitamin A-deficient diet feeding led to a marked increase of adiposity and to a small increase of body weight. Hypertrophy of white adipose tissue depots correlated with enhanced PPAR␥2 expression. Hypertrophy of BAT, in contrast, correlated with a decrease of PPAR␥2 expression that may contribute to the known reduced thermogenic potential of BAT under conditions of vitamin A restriction. Treatment with tRA triggered a reduction of adiposity and body weight that correlated with a down-regulation of PPAR␥2 expression in all adipose tissues. The effects of tRA were more pronounced in eWAT, where C/EBP␣ and ADD1/SREBP1c levels were also reduced. The response to tRA was impaired in the eWAT and BAT of animals fed the vitamin A-deficient diet. Discussion: The results emphasize the importance of retinoids as physiological regulators of adipose tissue development and function in intact animals.

show abstract

Section: Effects Of Tra Treatmentsupporting

confidence: 93%

Changes of Adiposity in Response to Vitamin A Status Correlate with Changes of PPARγ2 Expression

et al. 2001

View full text Add to dashboard Cite

show abstract

“…Overall, brown color in the normal group was the least; the most was seen in the IR group, followed by the IRϩVD3 group. Consistent with reported results in vitro (5) and in vivo (6), our results confirmed that 1,25-(OH)2D3 inhibited bone marrow pimelosis in bone marrow via down-regulation of expression of the PPAR␥ protein.…”

Section: Discussionsupporting

confidence: 93%

Inhibition of Adipogenesis Is Involved in the Protective Effects of 1,25-Dihydroxy Vitamin D3 on the Radiation-Injured Bone Marrow Microenvironment in Mice

Zhao

Chen

Zhu

et al. 2017

J Nutr Sci Vitaminol

View full text Add to dashboard Cite

SummaryTo explore the protective effects of 1,25-dihydroxy vitamin D3 (1,25-(OH)2D3) on the bone marrow microenvironment in mice after irradiation and the underlying molecular mechanisms, a total of 150 7-wk-old male BALB/c mice were randomly divided into a normal group, an irradiation (IR) group and an irradiationϩ1,25-(OH)2D3 (IRϩVD3) group. The mice in the IRϩVD3 group were treated with 6.0 Gy 60 Co␥ rays, and 1,25-(OH)2D3 (dissolved in DMSO, 2.5 g/kg) was administered once per day from 2 d before to 8 d after irradiation. Mice in the IR group were treated with the same dose of ␥ rays and an equal volume of DMSO. Subsequently, the body weights and the numbers of peripheral white blood cells (WBCs) were measured. Histological analysis of femur bone marrow was conducted to determine the proportion of adipose area as well. Finally, the expression of peroxisome proliferator-activated receptor-gamma (PPAR␥) in bone marrow was detected by immunohistochemistry. After irradiation, the percentage of adipose area in the bone marrow was significantly increased, and the WBC number and body weight were markedly reduced. Compared with irradiation alone, the co-administration of 1,25-(OH)2D3 with irradiation markedly attenuated radiation-induced adipogenesis in bone marrow, resulted in fewer bone marrow stromal cells expressing PPAR␥ and enhanced the recovery of body weight and WBCs. These results indicate that 1,25-(OH)2D3 could accelerate the recovery of body weight and WBCs in irradiated mice and protect the bone marrow by inhibiting radiation-induced adipogenesis via the down-regulation of PPAR␥ expression.

show abstract

“…So further study is needed to clarify the mechanism for the inhibition of PPARY expression. Interestingly, many other inhibitors of PPARY expression, such as vitamins, RA and 1,25(OH)2 D3 are fatsoluble (Hida et al, 1998). However, this compound of Maitake is water-soluble.…”

Section: Resultsmentioning

confidence: 99%

Effects of a Water-Soluble Bioactive Factor from Grifola frondosa, the Mushroom Maitake on Adipocyte Differentiation

Minamino

Yanaga

Masui

et al. 2004

FSTR

View full text Add to dashboard Cite

Counteraction of retinoic acid and 1,25-dihydroxyvitamin D3 on up-regulation of adipocyte differentiation with PPARγ ligand, an antidiabetic thiazolidinedione, in 3T3-L1 cells

Cited by 66 publications

References 18 publications

Changes of Adiposity in Response to Vitamin A Status Correlate with Changes of PPARγ2 Expression

Changes of Adiposity in Response to Vitamin A Status Correlate with Changes of PPARγ2 Expression

Inhibition of Adipogenesis Is Involved in the Protective Effects of 1,25-Dihydroxy Vitamin D3 on the Radiation-Injured Bone Marrow Microenvironment in Mice

Effects of a Water-Soluble Bioactive Factor from Grifola frondosa, the Mushroom Maitake on Adipocyte Differentiation

Contact Info

Product

Resources

About