2021
DOI: 10.1002/aocs.12559
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Countercurrent chromatography isolation of 11′‐γ‐tocomonoenol from pumpkin seed oil with detection of novel minor tocochromanols

Abstract: A non‐refined, organic pumpkin seed oil (PSO) was chosen for the isolation and structure verification of the rare vitamin E compound γ‐tocomonoenol (γ‐T1). Initial measurements indicated the presence of ~0.4 mg γ‐T1 per 100 g PSO. Saponification of ~2 L of PSO, followed by repeated countercurrent chromatography (CCC) with the solvent system n‐hexane/benzotrifluoride/acetonitrile (v/v/v) and silica gel column chromatography enabled the isolation of 6.8 mg γ‐T1 with a purity of 96.0% according to gas chromatogra… Show more

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Cited by 8 publications
(14 citation statements)
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“…T1s and T2s are less common in nature and can be found in lower concentration than Ts and T3s [ 14 , 16 ]. 11′-α-T1 was first isolated and purified from palm and rice bran oils in 1995 [ 69 ].…”
Section: Tocochomanols—history Terminology Structure and The Main Kno...mentioning
confidence: 99%
See 4 more Smart Citations
“…T1s and T2s are less common in nature and can be found in lower concentration than Ts and T3s [ 14 , 16 ]. 11′-α-T1 was first isolated and purified from palm and rice bran oils in 1995 [ 69 ].…”
Section: Tocochomanols—history Terminology Structure and The Main Kno...mentioning
confidence: 99%
“…Apart from this, in studies on T1s in plants with emphasis on thorough structure identification, only 11′-T1s have been structurally confirmed, and in cases where precise identification was not possible, detected T1s were assumed to be 11′-T1s ( Figure 8 ). T1s were identified in palm oil (11′-α-T1) [ 72 ], T3-rich fraction of palm oil (11′-α-T1) [ 52 ], countercurrent chromatography (CCC) fraction of palm oil (11′-α-, 11′-β-, and 11′-γ-T1) [ 73 ], in pumpkin seed oil and its CCC fraction (11′-α-, 11′-β-, 11′-γ-, and 11′-δ-T1) [ 16 ], leaves of Kalanchoe daigremontiana and Phaseolus coccineus (11′-β-, 11′-γ-, and 11′-δ-T1) [ 74 ], kiwi ( Actinidia chinensis ) peel and pulp (11′-δ-T1) [ 75 ], in cyanobacteria and microalgae (11′-α-T1 and MDT) [ 15 ] ( Figure 9 ). By analogy with T3s, T2s theoretically can have two unsaturated carbons at positions 3′ and 7′, 3′ and 11′ or 7′ and 11′ ( Figure 6 ).…”
Section: Tocochomanols—history Terminology Structure and The Main Kno...mentioning
confidence: 99%
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