Pump-As-Turbine (PAT) technology is a smart solution to produce energy in a sustainable way at small scale, e.g., through its exploitation in classical Water Distribution Networks (WDNs). PAT application may actually represent a suitable solution to obtain both pressure regulation and electrical energy production. This technology enables one to significantly reduce both design and maintenance costs if compared to traditional turbine applications. In this work, the potential hydropower generation was evaluated through laboratory tests focused on the characterization of a pump working in reverse mode, i.e., as a PAT. Both hydrodynamic (pressure and discharge) and mechanical (rotational speed and torque) conditions were varied during the tests, with the aim to identify the most efficient PAT configurations and provide useful hints for possible real-world applications. The experimental findings confirm the good performances of the PAT system, especially when rotational speed and water demand are, respectively, larger than 850 rpm and 8 L/s, thus leading to efficiencies greater than 50%. Such findings were applied to a small municipality, where daily distribution of pressure and discharge were recorded upstream of the local WDN, where a Pressure Reducing Valve (PRV) is installed. Under the hypothesis of PRV replacement with the tested PAT, three different scenarios were studied, based on the mean recorded water demand and each characterized by specific values of PAT rotational speed. The best performances were observed for the largest tested speeds (1050 and 1250 rpm), which lead to pressure drops smaller than those actually due to the PRV, thus guaranteeing the minimum pressure for users, but also to mechanical powers smaller than 100 W. When a larger mean water demand is assumed, much better performances are reached, especially for large speeds (1250 rpm) that lead to mechanical powers larger than 1 kW combined to head drops a bit larger than those observed using the PRV. A suitable design is thus fundamental for the real-world PAT application.