Coupled oxidation of ascorbic acid and haemoglobin

Lemberg, R.; Legge, J. W.; Lockwood, W. H.

doi:10.1042/bj0350328

Cited by 53 publications

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“…The first resembles both the "haematins a" of "cytochrome a" and a pigment mentioned by Lemberg (1938). The spectroscopy of its porphyrin suggests that it may have a group attached to one of the methene carbons.…”

Section: Discussionmentioning

confidence: 91%

“…The small yield of choleglobin resulting from the action of hydrogen peroxide on. haemoglobin, and the trifling yields from oxyhaemoglobin or methaemoglobin (Lemberg, 1941) may well be due to the catalatic powers of these solutions. (Cyanide inhibits the catalatic power of solutions of haemoglobin and oxyhaemoglobin, but is far less active against that of cyan-methaemoglobin.…”

Section: Discussionmentioning

confidence: 99%

“…Its constitution, like that of choleglobin, has yet to be established. Lemberg (1941) also showed that, in the absence of oxygen, hydrogen peroxide converts haemoglobin to choleglobin to the extent of 16 p.c. of the amount of the pigment present, a yield about one-half of that obtained by the incubation of oxyhaemoglobin with ascorbic acid.…”

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confidence: 95%

“…They named this pigment "pseudo-haemoglobin." Lemberg (1941) identified it as a cyanide compound of choleglobin, the protein of which was mainly denatured during its formation. Its constitution, like that of choleglobin, has yet to be established.…”

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confidence: 99%

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On the Decomposition of Methaemoglobin by Hydrogen Peroxide

Holden

1947

Aust J Exp Biol Med

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In general the derivatives of the blood pigment that contain native protein are not very susceptible to attack by hydrogen peroxide. The accompanying catalase, or their own inherent catalatic power, by decomposing the peroxide protects them from its action. Ferrous derivatives are not, to any great extent, oxidized to ferric. Three exceptions, however, have been recorded. Barkan and Schales (1938) showed that hydrogen peroxide, in the presence of potassium cyanide, acts on oxyhaemoglobin to form inter aUa, a greenish pigment with a strong absorption band in the red region of the spectrum. They named this pigment "pseudo-haemoglobin." Lemberg (1941) identified it as a cyanide compound of choleglobin, the protein of which was mainly denatured during its formation. Its constitution, like that of choleglobin, has yet to be established. Lemberg (1941) also showed that, in the absence of oxygen, hydrogen peroxide converts haemoglobin to choleglobin to the extent of 16 p.c. of the amount of the pigment present, a yield about one-half of that obtained by the incubation of oxyhaemoglobin with ascorbic acid. Brooks (1937) mentioned that in slightly acid solution and in the presence of sodium hyposulphite and 0-05 M sodium nitrite oxygen could convert nitric oxide haemoglobin to a greenish pigment whose oxidized form had an absorption band at 615 m/i, and its reduced form at 618 m/A. Nothing further was said about its properties or constitution. Since some hydrogen peroxide was probably produced during the reaction the pigment obtained may have been a mixture derived partly from oxidative disruption of the haemoglobin and partly from its decomposition by hydrogen peroxide.The experiments described in this paper are attempts to gain further insight into the class of reactions jnst outlined. They have now to be laid aside for a considerable time and are set down in hope that they will assist some other worker interested in the chemistry of the blood pigment. EXPERIMENTAL.Experiments on the action of hydrogen peroxide and sodium nitrite on oxyhaemoglobin yielded unsatisfactory mixtures. They showed, however, that only in weakly acid solution waa there any oxidative disruption of the. prosthetic group. In neutral or alkaline solution the products of the action of sodium nitrite on oxyhaemoglobin or methaemoglobin appear to exhibit the normal catalatic activity of solutions of the blood-pigment. In acid solution this is inhibited and, on the addition of hydrogen peroxide, a greenish solution results.The course of the reaction was studied in the following manner; 5 ml. of a 15 p.c. solution of autoxidation horse methaemoglobin at pH 5-3 were mixed with an equal volume of 1 p.e. sodium nitrite at the temperature of the laboratory; 1 ml. of approximately 1 p.c. hydrogen peroxide was added. Samples were removed after one minute, three minutes and each third minute thereafter for a quarter of an hour. These were at once dropped into tubes containing 4 p.c. sodium hydroxide and a little pyridine. These alkaline solutions were reduced...

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 95%

mentioning

confidence: 99%

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On the Decomposition of Methaemoglobin by Hydrogen Peroxide

Holden

1947

Aust J Exp Biol Med

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“…The latter combines not only witlh ferrohaemoglobin and a number of haemocliromo-* In order to avoid confusion which might otherwise ensue, the terms ferrihaemoglobin and ferrohaemoglobin are used throughout this paper instead of methaemoglobin and haemoglobin (cf. Coryell, Stitt & Pauliug, 1937). gens, but also with a series of compounds intermediate between these and the bile pigments (Lemberg, Legge & Lockwood, 1941). A significant difference between ferrohaemoglobin and 'total haemoglobin' may be regarded as being due to the presence of ferrihaemoglobin, especially as the technique for the determination of 'total haemoglobin does not embrace carbon monoxide haemoglobin.…”

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confidence: 99%