Coupling a gamma-ray detector with asymmetrical flow field flow fractionation (AF4): Application to a drug-delivery system for alpha-therapy

Huclier‐Markai, Sandrine; Du, Alicia Grivaud-Le; Montavon, Gilles; Mougin-Degraef, Marie; Barbet, Jacques

doi:10.1016/j.chroma.2018.08.065

Cited by 8 publications

(4 citation statements)

References 24 publications

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“…But by targeting elements like sulfur, present in biological molecules in a known amount, it was successfully employed for the quantification of monoclonal antibodies, free and particle-conjugated [135][136][137]. Recently, it was reported the first example of a γ-ray detector coupled to AF4 for the quantification of liposomes-encapsulated 212 Pb/ 212 Bi radionuclides for cancer treatment [138]. γ-ray detection allowed reaching concentrations in the fmol range, well below the limits of detection of other elemental analysis techniques.…”

Section: Choice Of the Detectormentioning

confidence: 99%

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Quattrini

Berrecoso

Crecente‐Campo

et al. 2021

Drug Deliv. and Transl. Res.

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The importance of polymeric nanocarriers in the field of drug delivery is ever-increasing, and the accurate characterization of their properties is paramount to understand and predict their behavior. Asymmetric flow field-flow fractionation (AF4) is a fractionation technique that has gained considerable attention for its gentle separation conditions, broad working range, and versatility. AF4 can be hyphenated to a plurality of concentration and size detectors, thus permitting the analysis of the multifunctionality of nanomaterials. Despite this potential, the practical information that can be retrieved by AF4 and its possible applications are still rather unfamiliar to the pharmaceutical scientist. This review was conceived as a primer that clearly states the “do’s and don’ts” about AF4 applied to the characterization of polymeric nanocarriers. Aside from size characterization, AF4 can be beneficial during formulation optimization, for drug loading and drug release determination and for the study of interactions among biomaterials. It will focus mainly on the advances made in the last 5 years, as well as indicating the problematics on the consensus, which have not been reached yet. Methodological recommendations for several case studies will be also included. Graphical abstract

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Section: Choice Of the Detectormentioning

confidence: 99%

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Quattrini

Berrecoso

Crecente‐Campo

et al. 2021

Drug Deliv. and Transl. Res.

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“…By adding MALS and DLS downstream of the separation, AF4 provided information on particle shape and morphology by measuring their shape factor, which allowed them to identify subtle differences in complexes between positively charged F-AmP peptides with both the negatively charged POs (palmitoyloleoylphosphatidylcholine) and DL-AUVs (dilaurylphosphatidylcholine-anionic unilamellar vesicles). Huclier-Markai et al, used AF4 with MALS and a gamma ray detector to monitor the liposome size together with the incorporation of the high energy alpha emitter ( 212 Bi) [ 55 ]. Considering 212Bi’s short half-life, it can only be delivered using labelled carrier particles (most notably, liposomes) that would rapidly accumulate in the target tumor.…”

Section: Applications Of Fff In Nanoparticle Drug Delivery Systemsmentioning

confidence: 99%

The Power of Field-Flow Fractionation in Characterization of Nanoparticles in Drug Delivery

Bian¹,

Gobalasingham

Purchel

et al. 2023

Molecules

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Asymmetric-flow field-flow fractionation (AF4) is a gentle, flexible, and powerful separation technique that is widely utilized for fractionating nanometer-sized analytes, which extend to many emerging nanocarriers for drug delivery, including lipid-, virus-, and polymer-based nanoparticles. To ascertain quality attributes and suitability of these nanostructures as drug delivery systems, including particle size distributions, shape, morphology, composition, and stability, it is imperative that comprehensive analytical tools be used to characterize the native properties of these nanoparticles. The capacity for AF4 to be readily coupled to multiple online detectors (MD-AF4) or non-destructively fractionated and analyzed offline make this technique broadly compatible with a multitude of characterization strategies, which can provide insight on size, mass, shape, dispersity, and many other critical quality attributes. This review will critically investigate MD-AF4 reports for characterizing nanoparticles in drug delivery, especially those reported in the last 10–15 years that characterize multiple attributes simultaneously downstream from fractionation.

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“…The development of optical trap flow cells for Raman microspectroscopy and coupling to AF4 and CF3 allowed the identification of latex and polymethylmethacrylate particles based on chemical composition, opening new possibilities in the analysis of nanoplastics in food and environmental matrixes [52]. The coupling on an γ-ray detector improved our capability of studying the interaction between radioactive components and the samples of biological/biomedical interest, such as liposomes, as well as their evolution over time [53]. Finally, the online coupling of NTA with AF4 [54] allowed us to obtain the particle count information in a simple in a fast way without suffering from the intrinsic problems of batch mode analysis (i.e., sample polydispersity, instrument sensitivity, and operator error).…”

Section: Novel Couplingsmentioning

confidence: 99%

Field-Flow Fractionation in Molecular Biology and Biotechnology

Giordani,

Marassi,

Placci

et al. 2023

Molecules

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Field-flow fractionation (FFF) is a family of single-phase separative techniques exploited to gently separate and characterize nano- and microsystems in suspension. These techniques cover an extremely wide dynamic range and are able to separate analytes in an interval between a few nm to 100 µm size-wise (over 15 orders of magnitude mass-wise). They are flexible in terms of mobile phase and can separate the analytes in native conditions, preserving their original structures/properties as much as possible. Molecular biology is the branch of biology that studies the molecular basis of biological activity, while biotechnology deals with the technological applications of biology. The areas where biotechnologies are required include industrial, agri-food, environmental, and pharmaceutical. Many species of biological interest belong to the operational range of FFF techniques, and their application to the analysis of such samples has steadily grown in the last 30 years. This work aims to summarize the main features, milestones, and results provided by the application of FFF in the field of molecular biology and biotechnology, with a focus on the years from 2000 to 2022. After a theoretical background overview of FFF and its methodologies, the results are reported based on the nature of the samples analyzed.

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Coupling a gamma-ray detector with asymmetrical flow field flow fractionation (AF4): Application to a drug-delivery system for alpha-therapy

Cited by 8 publications

References 24 publications

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

The Power of Field-Flow Fractionation in Characterization of Nanoparticles in Drug Delivery

Field-Flow Fractionation in Molecular Biology and Biotechnology

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