Revealing the spatial and temporal changing characteristics and key factors driving the relationship between the supply of and demand for ecosystem services (SDES) is paramount for the effective management of regional ecosystems and the rational allocation of natural resources. This study calculated the supply of ecosystem services (ESS) in Jiangsu Province, China, in 2000, 2010, and 2020, and quantitatively evaluated the ecosystem service demand (ESD) in those years by considering land development intensity, population density, and economic conditions. The ācoupling coordination degree and relative development degreeā (CDRD) model is introduced to explore the degree of coordination and change in the relationship of supply versus demand of ecosystem services, and then to reveal the impact of various factors on the spatial differentiation characteristics of the SDES. The results show that: 1) the ESS is high in the Taihu Lake Plain, the southwest of Huaihai area and the eastern coastal zone of Jiangsu Province, yet low in the northwest and middle of Huaihai area, and along the Yangtze River Plain. The overall distribution pattern of ESD and the coordinating degree of the SDES is high in the south and north of Jiangsu Province but low in the provinceās middle. 2) The degree of coordination for the SDES increased from 0.371 to 0.415, and the relationship between supply and demand changed from one of moderate imbalance to one of basic coordination. The imbalance of supply and demand is mainly concentrated in the Lixiahe area, coastal plain area, and middle of the Huaihe River Basin, for which the predominant type is a moderate imbalanceādemand lag. Meanwhile, the analysis of influencing factors shows that there is an obvious synergistic effect between different factors. The results of this research could contribute to sustainable ecosystem management and decision-making for the construction of ecological civilization in Jiangsu Province.