Coupling of narrow-bore liquid chromatography to thin-layer chromatography

Hofstraat, Johannes W.; Engelsma, M.; Nesse, R.J. van de; Brinkman, U.A.Th.; Gooijer, C.; Velthorst, N.H.

doi:10.1016/s0003-2670(00)86151-6

Cited by 29 publications

(7 citation statements)

References 16 publications

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“…LOG CONCENTRATION (fmol) efficient than in the glass. A similar observation was made for tetracene in ref 19.…”

Section: Resultssupporting

confidence: 84%

“…A spectroscopic technique that allows for resolution of such a zone into constituent components would be particularly valuable. With this and the versatility of FLN spectrometry in mind, we have initiated studies designed to explore the feasibility of coupling FLN with the 32P-postlabeling methodology. Fluorescence line narrowing for PAH sorbed on TLC plates had previously been reported by Hofstraat et al (18,19). In this paper we report FLN spectra and a detection limit of ~10 fmol for a deoxyguanosine adduct, formed from BP, sorbed on a TLC plate.…”

Section: Introductionsupporting

confidence: 78%

“…60, NO. 24, DECEMBER 15, 1988 had previously been reported by Hofstraat et al (18,19). In this paper we report FLN spectra and a detection limit of -10 fmol for a deoxyguanosine adduct, formed from BP, sorbed on a TLC plate.…”

supporting

confidence: 68%

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Fluorescence line narrowing spectrometry of nucleoside-polycyclic aromatic hydrocarbon adducts on thin-layer chromatographic plates

Jankowiak¹,

Hayes²,

Lu³

et al. 1988

Anal. Chem.

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Fluorescence llne narrowlng spectrometry (FLNS) is applied to a polycycllc aromatic hydrocarbon-nucleoside adduct sorbed on a thin-layer chromatography (TLC) plate. A caiibration curve over more than 5 decades of concentration Is presented and yleids a detectlon ilmlt of -10 fmol. The effects of nonphotochemkal hole burning (NPHB) are investigated and dlscussed. Results Indicate that FLN spectrometry can be Interfaced wlth the s2P-postlabeflng procedure to provide for high sensltlvlty and selectivity analysis of TLC spots assoclated with fluorescing nucleotide adducts.

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“…LOG CONCENTRATION (fmol) efficient than in the glass. A similar observation was made for tetracene in ref 19.…”

Section: Resultssupporting

confidence: 84%

Section: Introductionsupporting

confidence: 78%

See 1 more Smart Citation

Fluorescence line narrowing spectrometry of nucleoside-polycyclic aromatic hydrocarbon adducts on thin-layer chromatographic plates

Jankowiak¹,

Hayes²,

Lu³

et al. 1988

Anal. Chem.

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show abstract

“…Efficient deposition of the effluent by means of a spray-jet interface for RP eluents (aqueous methanol) has been already published. 1,2,[14][15][16][17] In the present paper we represent the first results on the use of ion-pair LC-TLC-SERRS for the separation and identification of four basic dyes. The main objective of this study was to study the effect of non-volatile ion-pair reagents commonly applied in the LC of dyes on the deposition process and on the SERRS spectra.…”

Section: Introductionmentioning

confidence: 97%

At-line coupling of surface-enhanced resonance Raman spectroscopy and reversed-phase ion-pair chromatography

Seifar¹,

Dijkstra²,

Brinkman³

et al. 1999

Anal. Commun.

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“…Interface requirements for coupling microcolumn HPLC or SFC to TLC are comparatively simple. With microbore HPLC columns that operate at flow rates of 5-100 ^L/min, the complete column effluent can be deposited on the TLC plate using a modified sprayjet band applicator (29,30). For example, Jaenchen used this approach to separate a mixture of 56 pesticides by a combination of reversed-phase microbore HPLC and normal-phase automated multiple development (31).…”

Section: Multidimensional and Multimodal Tlcmentioning

confidence: 99%