Coupling of Semiconductor Nanowires with Neurons and Their Interfacial Structure

Lee, Ki Young; Shim, Sojung; Kim, Il Soo; Oh, Hyun‐Taek; Kim, Sunoh; Ahn, Jae Pyeong; Park, Seung Han; Rhim, Hyewhon; Choi, Heon Jin

doi:10.1007/s11671-009-9498-0

Cited by 32 publications

(43 citation statements)

References 27 publications

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“…The research activity on semiconducting nanowires ͑NWs͒ is one of the most rapidly developing area in material science due the variety of important applications which, in the last years, has been envisaged in the fields of electronics, 1 optoelectronics, 2 nanomedicine, 3 and bioelectronics. 4 Due to their compatibility with the existing Si-based microelectronics, Si, 5 but also Ge and SiGe NWs have been the subject of intense experimental [6][7][8][9][10][11] and theoretical [12][13][14][15][16][17][18][19][20][21][22][23] investigations.…”

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confidence: 99%

Ab initiooptoelectronic properties of SiGe nanowires: Role of many-body effects

Palummo¹,

Amato

Ossicini³

2010

Phys. Rev. B

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The self-energy and electron-hole interaction corrections to the one-particle approximation for SiGe nanowires have been calculated for different geometries and diameters. We show that, at fixed nanowire diameter and orientation, the self-energy corrections for the SiGe nanowires can be obtained as a weighted average, on the relative composition of one type of atom with respect to the total numbers of atoms in the unit cell, of the corrections for the pure ͑Si and Ge͒ nanowires, thus circumventing cumbersome computations and allowing a direct and practical determination of the electronic band gap. Moreover we show that particular geometrical configurations are at the origin of an enhancement of the optical oscillator strength that should be important for optoelectronic applications.

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mentioning

confidence: 99%

Ab initiooptoelectronic properties of SiGe nanowires: Role of many-body effects

Palummo¹,

Amato

Ossicini³

2010

Phys. Rev. B

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“…As CVDgrown Si nanowires have high-aspect-ratios (<10 3 ) and are sufficiently rigid to be mechanically manipulated, their nanometer scale diameters and high-aspect-ratios allow them to access the interiors of living cells without an external force for penetration. Previous studies have shown that CVD-grown vertical nanowires spontaneously penetrate the cell membrane when cells are placed on top of the nanowires [26][27][28][29][30][31] . The biocompatibility of small diameter nanowires is a critical element for the in situ study of cellular processes of living cells 26 .…”

Section: Resultsmentioning

confidence: 99%

Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array

Yoo

Kwak

Kwon

et al. 2020

Sci Rep

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Continuous recording of intracellular activities in single cells is required for deciphering rare, dynamic and heterogeneous cell responses, which are missed by population or brief single-cell recording. Even if the field of intracellular recording is constantly proceeding, several technical challenges are still remained to conquer this important approach. Here, we demonstrate long-term intracellular recording by combining a vertical nanowire multi electrode array (VNMEA) with optogenetic stimulation to minimally disrupt cell survival and functions during intracellular access and measurement. We synthesized small-diameter and high-aspect-ratio silicon nanowires to spontaneously penetrate into single cells, and used light to modulate the cell's responsiveness. The light-induced intra-and extracellular activities of individual optogenetically-modified cells were measured simultaneously, and each cell showed distinctly different measurement characteristics according to the cell-electrode configuration. Intracellular recordings were achieved continuously and reliably without signal interference and attenuation over 24 hours. The integration of two controllable techniques, vertically grown nanowire electrodes and optogenetics, expands the strategies for discovering the mechanisms for crucial physiological and dynamic processes in various types of cells.Critical cellular dynamics, including transcriptional change, protein synthesis, receptor replacement, and synaptic plasticity in neural cells, take place over time periods ranging from several hours to days 1 . A common approach for discerning these cellular mechanisms in the single cell level is to measure its intracellular electrical activity using sharp microelectrodes or patch-clamping. However, as intracellular recording using these conventional electrodes is achieved by tearing the cell membrane, disruption of cell integrity limits the recording duration to several hours 2 , providing only brief 'snapshots' of cellular dynamics during limited experimental sessions 3 . Thus, such intermittent recordings have limitations in tracking single-unit activity over timescales relevant for most developmental and learning processes, or for pharmaceutical drug screening over long periods.Three-dimensional micro/nanostructure electrodes have shown superior feasibility for the electrophysiological study of single cells by accessing the cell interior and recent technologies allow simultaneous observations of the intracellular activity of individual cells in neuronal populations with high temporal/spatial resolution 4-17 . However, despite their advantages of high-sensitivity and minimal invasiveness, the reported time durations of intracellular recording using micro/nanostructure-based electrodes have not exceeded 80 min 4 . These intracellular recordings have been demonstrated by employing one of two agents: (i) external poration force achieved by a burst of electrical (electroporation) or optical (optoporation) 4 pulses applied through electrodes to increase the permeability ...

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“…Moreover, our previous study indicated that Si nanowires are bio-compatible to excitable cells (hippocampal neurons) and are thus safe for interfacing [26]. …”

Section: Resultsmentioning

confidence: 99%

“…Based on the findings of previous studies [26,27], the length (3 to 4 μm) and the diameter (60 to 100 nm) of the nanowires were set to optimum cell interfacing conditions.…”

Section: Methodsmentioning

confidence: 99%

Vertical nanowire probes for intracellular signaling of living cells

Lee

Kim

et al. 2014

Nanoscale Res Lett

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The single living cell action potential was measured in an intracellular mode by using a vertical nanoelectrode. For intracellular interfacing, Si nanowires were vertically grown in a controlled manner, and optimum conditions, such as diameter, length, and nanowire density, were determined by culturing cells on the nanowires. Vertical nanowire probes were then fabricated with a complimentary metal-oxide-semiconductor (CMOS) process including sequential deposition of the passivation and electrode layers on the nanowires, and a subsequent partial etching process. The fabricated nanowire probes had an approximately 60-nm diameter and were intracellular. These probes interfaced with a GH3 cell and measured the spontaneous action potential. It successfully measured the action potential, which rapidly reached a steady state with average peak amplitude of approximately 10 mV, duration of approximately 140 ms, and period of 0.9 Hz.

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Coupling of Semiconductor Nanowires with Neurons and Their Interfacial Structure

Cited by 32 publications

References 27 publications

Ab initiooptoelectronic properties of SiGe nanowires: Role of many-body effects

Ab initiooptoelectronic properties of SiGe nanowires: Role of many-body effects

Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array

Vertical nanowire probes for intracellular signaling of living cells

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