Courtship song genes and speciation in sand flies

Oliveira, Sabrina Gomes de; Bottecchia, Marcelle; Bauzer, Luíz Guilherme Soares da Rocha; Souza, Nataly A.; Ward, R. D.; Kyriacou, Charalambos P.; Peixoto, Alexandre A.

doi:10.1590/s0074-02762001000300022

Cited by 19 publications

(16 citation statements)

References 24 publications

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“…The values of the Tajima parameter (D) were -0.73333 (P > 0.10) for the field population and -0.30187 (P > 0.10) for the laboratory colony, so that selection can be discounted as being responsible for the diversity encountered (Table 1) and the F ST value calculated between both populations was 0.37179 (P = 0.05), suggesting that there is a considerable degree of genetic differentiation between them. Similar values have been discovered when comparing divergent geographic populations in insect vectors of pathogens (Oliveira et al 2001). The analyses of maximum probability of the cytochrome b gene under the model HKY (Hasegawa et al 1985) produced the tree shown in Fig.…”

Section: Resultssupporting

confidence: 73%

Mitochondrial DNA divergence between wild and laboratory populations of Anopheles albimanus Wiedemann (Diptera: Culicidae)

et al. 2005

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Divergencia del ADN Mitocondrial entre Poblaciones del Campo y de Colonia de Anopheles albimanusWiedemann (Diptera: Culicidae)RESUMEN -Las colonias de laboratorio facilitan el estudio de los insectos vectores; sin embargo, se ha sugerido que tales colonias de insectos no son representativas de las poblaciones naturales, llevando en algunos casos, a interpretaciones erróneas respecto a la variación intraespecífica entre los individuos. En el presente estudio se evaluó la variabilidad del gen mitocondrial citocromo b entre una colonia de laboratorio de Anopheles albimanus Wiedemann fundada hace 20 años y la población del campo de la cual se derivó. Los análisis revelan la presencia de cinco y tres haplotipos en las poblaciones del campo y la colonia, respectivamente; los individuos del campo presentaron una mayor variabilidad que los de la colonia basada en el numero de sitios polimórficos, la diversidad haplotípica, la diversidad nucleotídica y el valor promedio de las diferencias nucleotídicas. El promedio y el número neto de sustituciones nucleotídicas por sitio entre las poblaciones y los valores calculados para el F ST (0,37179, P = 0.05) indican que existe un considerable grado de diferenciación genética entre ellas; el árbol filogenético muestra que los haplotipos de la colonia parecen derivarse de las poblaciones del campo. Estos resultados sugieren una mayor variabilidad genética existente entre los especimenes del campo en comparación con los individuos de la colonia debido en parte, al largo tiempo de colonización. PALABRAS-CLAVE: Variabilidad genética, malaria, mosquitoABSTRACT -Studies of insect vectors may be facilitated by using laboratory colonies. However, it has been suggested that the colony insects are not representative of natural populations, sometimes yealding to erroneous interpretations of the intraspecific genetic variation between the individuals. In the present study the variability of the mitochondrial gene cytochrome b was evaluated among a closed laboratory colony of Anopheles albimanus that was founded 20 years ago and the field population from which it was derived. The analyses revealed the presence of five and three nucleotide haplotypes in the wild and colony populations, respectively. Wild individuals presented greater variability than those of the colony based on the number of polymorphic sites, haplotype diversity, nucleotide diversity and mean values of nucleotide differences. The mean and net numbers of nucleotide substitutions per site between populations and the significant F ST value calculated (0,37179, P = 0.05) indicate that there is a considerable degree of genetic differentiation between them. The phylogenetic tree showed that the colony haplotypes appear to be derived from the wild population. These results suggest a great genetic variability in wild specimens compared with the laboratory ones as a consequence of a long time of colonization.

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Section: Resultssupporting

confidence: 73%

Mitochondrial DNA divergence between wild and laboratory populations of Anopheles albimanus Wiedemann (Diptera: Culicidae)

et al. 2005

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“…Most commonly, these markers are sequenced alone or coupled to one or two others. The most frequent are genes coding for lovesongs: period (Bauzer et al, 2002a,b;Mazzoni et al, 2002Mazzoni et al, , 2006Mazzoni et al, , 2008Araki et al, 2009;Salomon et al, 2010;Vigoder et al, 2010) and cacophony (Oliveira et al, 2001;Lins et al, 2002;Bottecchia et al, 2004;Mazzoni et al, 2008;Depaquit et al, 2014) Valderrama et al, 2014). Other markers have only been used one time each: copulatory courtship songs: , Reverse transcriptase (Booth et al, 1991), Non-LTR retrotransposons (Booth et al, 1994), para genes: , Ca1D , Rp49 , maxadilan (Yin et al, 2000) and SP15-Saliva protein (Elnaiem et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

Molecular systematics applied to Phlebotomine sandflies: Review and perspectives

Depaquit

2014

Infection, Genetics and Evolution

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“…This fragment includes an intron missing in Drosophila (Peixoto et al. , 1997) that is polymorphic in L. longipalpis (Oliveira et al. , 2001).…”

Section: Introductionmentioning

confidence: 99%

Molecular evolution of thecacophonyIVS6 region in sandflies

Lins

Oliveira

Souza

et al. 2002

Insect Molecular Biology

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A number of insects produce acoustic signals during courtship. Genes involved in the control of the courtship song are particularly interesting from an evolutionary viewpoint because interspecific variation in this signal is potentially important as a reproductive isolation mechanism and, as a consequence, in the speciation process. The cacophony gene was identified by a mutation affecting the "lovesong" in Drosophila melanogaster. Phlebotomine sandflies (Diptera: Psychodidae) also produce acoustic stimuli during courtship and therefore cacophony can be used as an interesting molecular marker in evolutionary studies in these important disease vectors. In this paper we have studied the molecular evolution of the IVS6 region of cacophony in sandflies. We compared the level of divergence in the exon sequences encoding this conserved domain in Drosophila and Phlebotomines. We also analysed the high level of variation in an intron that is present in sandflies but that was lost in Drosophila during evolution. The available cacophony sequences were also used for a phylogenetic analysis of some species of the Neotropical genus Lutzomyia.

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Courtship song genes and speciation in sand flies

Abstract: Lutzomyia longipalpis (Lutz & Neiva, 1912)

Cited by 19 publications

References 24 publications

Mitochondrial DNA divergence between wild and laboratory populations of Anopheles albimanus Wiedemann (Diptera: Culicidae)

Mitochondrial DNA divergence between wild and laboratory populations of Anopheles albimanus Wiedemann (Diptera: Culicidae)

Molecular systematics applied to Phlebotomine sandflies: Review and perspectives

Molecular evolution of thecacophonyIVS6 region in sandflies

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