Covalent attachment of aptamer onto nanocomposite as a high performance electrochemical sensing platform: Fabrication of an ultra-sensitive ibuprofen electrochemical aptasensor

Roushani, Mahmoud; Shahdost-fard, Faezeh

doi:10.1016/j.msec.2016.05.099

Cited by 34 publications

(14 citation statements)

References 45 publications

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“…In aptasensors, redox probe is charged oppositely to aptamer so that electrostatic repulsion makes the interface very sensitive to the shielding of aptamer charge or its compensation dye to target interactions. Such impedimetric sensors were reported for determination of other low‐molecular analytes, e. g., patulin , ochratoxin A , kanamycin , ibuprofen etc. The use of conductive polymers like PANI or poly( p ‐phenylene diamine) increases sensitivity of the detection due to pH‐ or charge dependency of underlying layer.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

et al. 2019

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Mycotoxins are highly toxic metabolites of some fungi that frequently contaminate water, food and feed and hence cause several human and animal diseases. In this work, a new approach to the fast and reliable determination of aflatoxin M1 (AFM1) in water and milk has been proposed with reagent free protocol of signal measurement. For this purpose, DNA aptamer selective to AFM1 was entrapped between two thin layers of polyaniline (PANI) electrodeposited on glassy carbon electrode. The incubation of the aptasensor in the AFM1 solution results in remarkable decrease of the PANI intrinsic activity monitored by direct current voltammetry or electrochemical impedance spectroscopy. Appropriate calibration curves were linear in the range from 3 to 90 ng/L with limit of detection (LOD) 1–5 ng/L depending on the measurement mode. Mechanism of signal generation involves shielding electrostatic interactions between the PANI and aptamer in the surface layer and variation of its redox activity attributed to the emeraldine form of PANI. Selectivity of the response was proved by similar experiments with aflatoxin B1 and ochratoxin A and by comparison of the results with those obtained with non‐specific aptamer in the sensing layer. Simple protocol for milk pretreatment has been proposed for reliable detection of AFM1 on the level of its threshold limited values (20 ng/L).

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Section: Resultsmentioning

confidence: 99%

Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

et al. 2019

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“…Still, in general, the most sensitive methods comprised biological entities for highly specific biorecognition. For instance, biosensors with immobilized antibodies (immunosensor) or DNA/RNA fragments (aptasensor) achieved considerably lower values of LOD, reaching concentrations between 10 −5 to 10 −8 μmol L −1 , either using graphene [ 86 , 90 ], or CNTs [ 116 , 118 ] as signal enhancer and substrate for biomolecules attachment. It is worth noting that only one work [ 126 ] resorted to enzymatic biorecognition of pharmaceuticals, which shows a clear gap since many pharmaceutical drugs have phenolic derivatives that can act as substrates of enzymes such as laccase, tyrosinase, or hydrogen peroxidase [ 152 ].…”

Section: Overall Comparison Between Reported Nanostructured Carbonmentioning

confidence: 99%

“…Tap water was analyzed as real sample with no traces of diclofenac being found [117]. It seems evident that the most sensitive (bio)sensors are based in highly specific recognition elements such as MIP [122,128] or biological entities involving aptamers [118] and antibodies [104,116,125]. In this way, the lowest LOD (6.2 × 10 −9 µmol L −1 ) corresponded to a MIP sensor developed for 17β-estradiol detection in environmental waters.…”

Section: Carbon Nanotubesmentioning

confidence: 99%

Application of Nanostructured Carbon-Based Electrochemical (Bio)Sensors for Screening of Emerging Pharmaceutical Pollutants in Waters and Aquatic Species: A Review

et al. 2020

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Pharmaceuticals, as a contaminant of emergent concern, are being released uncontrollably into the environment potentially causing hazardous effects to aquatic ecosystems and consequently to human health. In the absence of well-established monitoring programs, one can only imagine the full extent of this problem and so there is an urgent need for the development of extremely sensitive, portable, and low-cost devices to perform analysis. Carbon-based nanomaterials are the most used nanostructures in (bio)sensors construction attributed to their facile and well-characterized production methods, commercial availability, reduced cost, high chemical stability, and low toxicity. However, most importantly, their relatively good conductivity enabling appropriate electron transfer rates—as well as their high surface area yielding attachment and extraordinary loading capacity for biomolecules—have been relevant and desirable features, justifying the key role that they have been playing, and will continue to play, in electrochemical (bio)sensor development. The present review outlines the contribution of carbon nanomaterials (carbon nanotubes, graphene, fullerene, carbon nanofibers, carbon black, carbon nanopowder, biochar nanoparticles, and graphite oxide), used alone or combined with other (nano)materials, to the field of environmental (bio)sensing, and more specifically, to pharmaceutical pollutants analysis in waters and aquatic species. The main trends of this field of research are also addressed.

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“…Several methods have already been reported for quantitative determination of KP, including liquid chromatography-mass spectrometry [ 3 ], UV-fluorescence [ 4 ], ion chromatography [ 5 ], flow injection with chemiluminescence [ 6 ], or electrochemical detection [ 7 ]. Both chromatographic and non-chromatographic techniques usually require rigorous sample preparation and expensive extraction methods (including solid-phase extraction) when real samples are considered.…”

Section: Introductionmentioning

confidence: 99%

“…A similar electrode was utilized by Ghoneim and Tawfik [ 10 ], and in a Britton-Robinson buffer (pH 2.0) the LOD was 0.10 ng mL –1 . Next, a setup containing glassy carbon (GC) electrode with multiwalled carbon nanotubes/ionic liquid/chitosan composite for covalent immobilization of the ibuprofen by specific aptamer was proposed [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Optical Detection of Ketoprofen by Its Electropolymerization on an Indium Tin Oxide-Coated Optical Fiber Probe

Bogdanowicz

Niedziałkowski

Sobaszek

et al. 2018

Sensors

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In this work an application of optical fiber sensors for real-time optical monitoring of electrochemical deposition of ketoprofen during its anodic oxidation is discussed. The sensors were fabricated by reactive magnetron sputtering of indium tin oxide (ITO) on a 2.5 cm-long core of polymer-clad silica fibers. ITO tuned in optical properties and thickness allows for achieving a lossy-mode resonance (LMR) phenomenon and it can be simultaneously applied as an electrode in an electrochemical setup. The ITO-LMR electrode allows for optical monitoring of changes occurring at the electrode during electrochemical processing. The studies have shown that the ITO-LMR sensor’s spectral response strongly depends on electrochemical modification of its surface by ketoprofen. The effect can be applied for real-time detection of ketoprofen. The obtained sensitivities reached over 1400 nm/M (nm·mg−1·L) and 16,400 a.u./M (a.u.·mg−1·L) for resonance wavelength and transmission shifts, respectively. The proposed method is a valuable alternative for the analysis of ketoprofen within the concentration range of 0.25–250 μg mL−1, and allows for its determination at therapeutic and toxic levels. The proposed novel sensing approach provides a promising strategy for both optical and electrochemical detection of electrochemical modifications of ITO or its surface by various compounds.

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Covalent attachment of aptamer onto nanocomposite as a high performance electrochemical sensing platform: Fabrication of an ultra-sensitive ibuprofen electrochemical aptasensor

Cited by 34 publications

References 45 publications

Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

Electrochemical Aptasensor with Layer‐by‐layer Deposited Polyaniline for Aflatoxin M1 Voltammetric Determination

Application of Nanostructured Carbon-Based Electrochemical (Bio)Sensors for Screening of Emerging Pharmaceutical Pollutants in Waters and Aquatic Species: A Review

Optical Detection of Ketoprofen by Its Electropolymerization on an Indium Tin Oxide-Coated Optical Fiber Probe

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