Covalent attachment of cell-adhesive peptide Gly-Arg-Gly-Asp (GRGD) to poly(etheretherketone) surface by tailored silanization layers technique

Covalently functionalized poly(etheretherketone) implants with osteogenic growth peptide (OGP) to improve osteogenesis activity

“…On the basis of these observations, it can be safely concluded that surface hydroxylation of PEEK sheet was successfully achieved through selective carbonyl reduction. 10…”

“…7 Currently, in order to overcome the bio-inertness of PEEK, a large number of researches have been carried out and multiple strategies were found. [7][8][9][10] It has been demonstrated that the surface chemistry and structures are prime factors governing cell adhesion and growth. 11 One of the most important methods is the biofunctionalization of PEEK by surface modication with bioactive molecules, peptide or growth factors.…”

“…11 One of the most important methods is the biofunctionalization of PEEK by surface modication with bioactive molecules, peptide or growth factors. 9,10,12 The osteogenic growth peptide (OGP, isoelectric point ¼ 11.4, M w ¼ 1.5 kDa, Ala-Leu-Lys-Arg-Gln-Gly-Arg-Thr-Leu-Tyr-Gly-Phe-Gly-Gly) is a naturally occurring tetradecapeptide identical to the C-terminal amino acid sequence 89-102 of histone H4 (H4). 13 OGP in high abundance occurs physiologically in human and rodent serum and in serum-free medium of osteoblastic and broblastic cells.…”

Surface phosphonation treatment shows dose-dependent enhancement of the bioactivity of polyetheretherketone

“…21,22 As such, the tailoring of surface chemicals such as in the case of covalent graing of functional groups or biomolecules without the hindrance of the detachment is a promising alternative to coating techniques. Our groups and others have reported that implants with incorporated functional groups such as carboxyl, 23,24 sulfonic groups, 25 amino 26 and phosphonate groups, 27,28 or biomolecules such as RGD peptides 29 and antimicrobial peptides 30 exhibit up-regulated bone cells osteogenic activity in vitro and osseointegration in vivo.…”

Enhanced Anti-corrosion Ability and Biocompatibility of PLGA Coatings on MgZnYNd Alloy by BTSE-APTES Pre-treatment for Cardiovascular Stent

“…It was reported by Zheng et al that MC3T3-E1 attachment, stretching and proliferation were effectively enhanced on GRGD-modified PEEK surface by tailored silanization technique [76] . Besides, silane-coupling agent aminopropyl triethoxysilane was used as a very effective system for collagen immobilizing onto stainless steel to improve human mesenchymal stem cells adhesion and proliferation [27] .…”