Covalent attachment of trypsin on plasma polymerized allylamine

Abbas, Abdennour; Vercaigne-Marko, Dominique; Supiot, Philippe; Bocquet, B.; Vivien, Céline; Guillochon, Didier

doi:10.1016/j.colsurfb.2009.06.007

Cited by 34 publications

(23 citation statements)

References 52 publications

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“…As a result, GA plays a role of a crosslinker between the amine groups of p-ATP molecules and the amine moieties on the side chains of the protein templates by forming unstable imine bonds in basic pH buffer solution (Figure 1a). [24] Following the immobilization of the template, the organo-siloxane monomers trimethoxypropylsilane (TMPS) and (3-aminopropyl)trimethoxysilane (APTMS), which are hydrolytically unstable, are co-polymerized onto the modified AuNR surface. While the Si-C bond and aminopropyl group cannot be cleaved, the ethoxy groups of APTMS and methoxy groups of TMPS undergo rapid hydrolysis to produce ethanol, methanol and trisilanols (Figure 1b).…”

Section: Resultsmentioning

confidence: 99%

Hot Spot‐Localized Artificial Antibodies for Label‐Free Plasmonic Biosensing

Abbas

Tian

Morrissey

et al. 2012

Adv Funct Materials

Self Cite

101

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The development of biomolecular imprinting over the last decade has raised promising perspectives in replacing natural antibodies with artificial antibodies. A significant number of reports have been dedicated to imprinting of organic and inorganic nanostructures, but very few were performed on nanomaterials with a transduction function. Herein we describe a relatively fast and efficient plasmonic hot spot-localized surface imprinting of gold nanorods using reversible template immobilization and siloxane co-polymerization. The technique enables a fine control of the imprinting process at the nanometer scale and provides a nanobiosensor with high selectivity and reusability. Proof of concept is established by the detection of neutrophil gelatinase-associated lipocalin (NGAL), a biomarker for acute kidney injury, using localized surface plasmon resonance spectroscopy. The work represents a valuable step towards plasmonic nanobiosensors with synthetic antibodies for label-free and cost-efficient diagnostic assays. We expect that this novel class of surface imprinted plasmonic nanomaterials will open up new possibilities in advancing biomedical applications of plasmonic nanostructures.

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Section: Resultsmentioning

confidence: 99%

Hot Spot‐Localized Artificial Antibodies for Label‐Free Plasmonic Biosensing

Abbas

Tian

Morrissey

et al. 2012

Adv Funct Materials

Self Cite

101

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“…As the qualitative analysis of the amines were in good agreement with the amine quantification by AO II, we concluded that the PPAam coating fabricated under 30 W present the highest amine density in these groups. In this low energy process, the monomer structure with the primary amines is best retained, whereas more fragmentation occurs in the high energy plasma polymerization process, where primary amine groups have the tendency of transforming into nitrile, amide, or imine functional groups …”

Section: Resultsmentioning

confidence: 99%

Constructing Biomimic Catalytic Coating with Controlled Nitric Oxide Release Properties by Immobilizing 3,3-Diselenodipropionic Acid on Plasma Polymerized Allylamine Film

Yang

et al. 2014

Plasma Process. Polym.

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Nitric oxide (NO) is a signaling molecule that plays a pivotal role in the diagnosis, treatment, and prevention of life threatening cardiovascular disorders. In this work, a facile method to construct catalytic coating with controlled NO release properties has been developed. The process involves in the first-step the deposition of plasma polymerized allylamine coating to introduce amine groups onto the titanium foils, followed by covalently immobilizing 3,3-diselenodipropionic acid (SeDPA), which is an organoselenium compound mimicking glutathione peroxidase-like catalytic production of NO. The NO release correlated positively with the -Se-Se-content in the functionalized film. After 15 d exposure to an NO donor, the -Se-Se-group was well retained with good biostability and ability of NO generation.

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“…Since cells interact with these adsorbed molecules and not with the underlying material surface, the properties of the protein layer influence cellular behaviour and control biocompatibility (Ratner and Bryant, 2004). Allylamine plasma polymer seems to feature high adsorptivity, as has been exemplarily demonstrated for the enzyme trypsin (Abbas et al, 2009). This could link the polyelectrolyte properties of PPAAm with a higher adsorption of biomolecules from medium and serum components (such as fibronectin) that in turn favour cell adhesion by means of integrin-mediated focal adhesion complexes.…”

Section: Introductionmentioning

confidence: 81%

Osteoblasts with impaired spreading capacity benefit from the positive charges of plasma polymerised allylamine

Kunz

Rebl²,

Quade³

et al. 2015

eCM

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Bone diseases such as osteoporosis, osteoarthritis and rheumatoid arthritis, impinge on the performance of orthopaedic implants by impairing bone regeneration. For this reason, the development of effective surface modifications supporting the ingrowth of implants in morbid bone tissue is essential. Our study is designed to elucidate if cells with restricted cell-function limiting adhesion processes benefit from plasma polymer deposition on titanium. We used the actin filament disrupting agent cytochalasin D (CD) as an experimental model for cells with impaired actin cytoskeleton. Indeed, the cell's capacity to adhere and spread was drastically reduced due to shortened actin filaments and vinculin contacts that were smaller. The coating of titanium with a positively charged nanolayer of plasma polymerised allylamine (PPAAm) abrogated these disadvantages in cell adhesion and the CD-treated osteoblasts were able to spread significantly. Interestingly, PPAAm increased spreading by causing enhanced vinculin number and contact length, but without significantly reorganising actin filaments. PPAAm with the monomer allylamine was deposited in a microwave-excited low-pressure plasma-processing reactor. Cell physiology was monitored by flow cytometry and confocal laser scanning microscopy, and the length and number of actin filaments was quantified by mathematical image processing. We showed that biomaterial surface modification with PPAAm could be beneficial even for osteoblasts with impaired cytoskeleton components. These insights into in vitro conditions may be used for the evaluation of future strategies to design implants for morbid bone tissue.

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Covalent attachment of trypsin on plasma polymerized allylamine

Cited by 34 publications

References 52 publications

Hot Spot‐Localized Artificial Antibodies for Label‐Free Plasmonic Biosensing

Hot Spot‐Localized Artificial Antibodies for Label‐Free Plasmonic Biosensing

Constructing Biomimic Catalytic Coating with Controlled Nitric Oxide Release Properties by Immobilizing 3,3-Diselenodipropionic Acid on Plasma Polymerized Allylamine Film

Osteoblasts with impaired spreading capacity benefit from the positive charges of plasma polymerised allylamine

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