Covalent bound sensing layers on surface acoustic wave (SAW) biosensors

Barie, N.; Rapp, M.

doi:10.1016/s0956-5663(01)00198-1

Cited by 55 publications

(25 citation statements)

References 28 publications

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“…BIA based on different physical sensor systems, e.g. surface plasmon resonance (SPR) [44], reflectometric interference spectroscopy (RIFS) [45], or surface acoustic wave technology (SAW) [46], coupled either off-line or on-line to mass spectrometry BIA-MS, offers a promising tool in BLIA [47,48]. Fig.…”

Section: Discussionmentioning

confidence: 99%

Approaches to bioresponse-linked instrumental analysis in water analysis

Brenner‐Weiß¹,

Obst²

2003

Analytical and Bioanalytical Chemistry

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A new concept based on hyphenation of biotests, for biological selection, and chemical analysis is introduced for water analysis. Biomolecular recognition components such as receptors, enzymes, and nucleic acids integrated in biological reaction chains are used for binding and selective enrichment of known and unknown biologically active substances in water samples; this is followed by identification and quantitation. The coupling of biomolecular recognition and binding to chemical analysis can be achieved either in discrete analytical steps, e.g. binding and elution of bioactive ligands from affinity columns followed by chemical analysis, or by methods capable of monitoring the binding of the ligand and simultaneous verification of its identity. This analytical strategy, denoted bioresponse-linked instrumental analysis (BLIA), enables detection of potential biological effects and identification of the analyte causing these effects. Several examples are presented.

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Section: Discussionmentioning

confidence: 99%

Approaches to bioresponse-linked instrumental analysis in water analysis

Brenner‐Weiß¹,

Obst²

2003

Analytical and Bioanalytical Chemistry

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“…Self-assembled monolayer of anti-insulin antibodies was formed on the upper (liquid facing) side of the cantilever via amino-group formed peptide coupling. 24,25 Cantilevers functionalized in such a way was exposed to insulin solutions from 68 pM (0.4 ng ml 21 ) to 1.08 nM (6.3 ng ml…”

Section: Introductionmentioning

confidence: 99%

Application of a new microcantilever biosensor resonating at the air–liquid interface for direct insulin detection and continuous monitoring of enzymatic reactions

et al. 2012

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Here we describe the application of a recently developed high-resolution microcantilever biosensor resonating at the air-liquid interface for the continuous detection of antigen-antibody and enzymesubstrate interactions. The cantilever at the air-liquid interface demonstrated 50% higher quality factor and a 5.7-fold increase in signal-to-noise-ratio (SNR) compared with one immersed in the purified water. First, a label-free detection of a low molecular weight protein (insulin, 5.8 kDa) in physiological concentration was demonstrated. The liquid facing side of the cantilever was functionalized by coating its surface with insulin antibodies, while the opposite side was exposed to air. The meniscus membrane at the micro-slit around the cantilever sustained the liquid in the microchannel. After optimizing the process of surface functionalization, the resonance frequency shift was successfully measured for insulin solutions of 0.4, 2.0, and 6.3 ng ml 21. To demonstrate additional application of the device for monitoring enzymatic protein degradation, the liquid facing microcantilever surface was coated with human recombinant SOD1 (superoxide dismutase 1) and exposed to various concentrations of proteinase K solution, and the kinetics of the SOD1 digestion was continuously monitored. The results showed that it is a suitable tool for sensitive protein detection and analysis.

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“…Biosensor research indicates that quartz (the crystalline form of SiO 2 ), a piezoelectric crystal with an inert surface, can be used for the immobilization of proteins for biodetection (Barie and Rapp, 2001). Zinc oxide (ZnO), is a material system with a higher piezoelectric coefficient (0.43 C/cm 2 ), and has a more reactive surface.…”

Section: Introductionmentioning

confidence: 99%