Covalent coupling of cholic acid to aminohexylamino-Sepharose 4B and its use in affinity chromatography of serum albumin

Cholesterol-lowering effect of rice bran protein containing bile acid-binding proteins

“…Sodium cholate hydrate (Sigma-Aldrich) was coupled to EAH Sepharose 4B (GE Healthcare, Tokyo, Japan) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (Dojindo Laboratories, Kumamoto, Japan)-HCl (pH 6.4) according to the procedure described by Pattinson et al 16) RBP was dissolved in 0.02% NaN 3 containing 10 mM Tris-HCl (pH 8.0). The RBP solution was filtered by membrane filters with a 0.20 μm pore size (Advantec, Tokyo, Japan).…”

Bile Acid-binding Protein from Soybean Seed: Isolation, Partial Characterization and Insulin-stimulating Activity

“…Cholic acid was coupled to aminohexyl-Sepharose 4B using ethyl(dimethylpropyl}carbodiimide at pH 6.2~6.4, according to the procedure of Pattinson et al 8 ) Analytical procedures. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE) was carried out at a gel concentration of 8% in slab gels (0.2 x 14 x 14cm), using 0.1 M NaHPOc NaH2P04 buffer at pH 7.0, as described by Moriyama et al 9 ) The binding of bile salts to proteins was measured by the method of equilibrium dialysis in three-ch~mbered Lucite cells separated by a Visking membrane.…”

Rat liver glutathione S-transferases. A study of the structure of the basic YbYb-containing enzymes

“…The transferases have been widely studied because of their ability to bind hydrophobic nonsubstrate ligands (Ketley et al, 1975;Wolkoff et al, 1978). During the present study cholate-aminohexyl-Sepharose 4B (Pattinson et al, 1980) was used to purify a number of transferases. Pattinson (1981) reported that transferases C, A and AA bound to this affinity matrix.…”