Covalent Electropolymerisation of Glucose Oxidase in Polypyrrole

Yon-Hin, B.F.Y.; Lowe, Christopher R.

doi:10.1016/b978-1-85617-242-4.50049-5

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…As already reported for electropolymerization on a Pt electrode in aqueous solution, 25 the potential at which pyrrole-3-acetic acid oxidation begins is comparable to that observed for pyrrole. Therefore,ˇ-substitution has little effect on the electrochemical properties of the aromatic nucleus, in contrast to that observed in the case of pyrrole-3-carboxylic acid, whose oxidation peak was shifted towards more positive potentials.…”

supporting

confidence: 81%

Surface (XPS, SIMS) chemical investigation on poly(pyrrole-3-acetic acid) films electrosynthesized on Ti and TiAlV substrates for the development of new bioactive substrates

Giglio

Calvano

Losito

et al. 2005

Surf. Interface Anal.

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Electropolymerization of pyrrole-3-acetic acid was performed by cyclic voltammetry on titanium and Ti90Al6V4 substrates with the aim of developing a multilayer structure for applications in advanced biomaterials. The polymeric films obtained were characterized by both XPS and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Information on the poly(pyrrole-3-acetic acid) (PPy-3-acetic) surface structure was achieved by a detailed XPS analysis of C 1s and N 1s signals. The number of COOH groups was quantified by XPS coupled to a chemical derivatization reaction in which esterification with trifluoroethanol was exploited so that the presence of fluorine (or the CF 3 component in C 1s spectra) could be used as a marker for COOH groups. As a result, it was found that more than 90% of the monomer units along PPy-3-acetic chains bear carboxylic functionalities, of which 60% are protonated and 40% are present as carboxylate groups. Some decarboxylation occurs with film ageing.The PPy-3-acetic films were also investigated by ToF-SIMS in the negative ion mode, thus obtaining, for the first time, interesting information on the structure of the top surface layers of a polymer belonging to the polypyrrole family. In particular, clusters of peaks related to PPy-3-acetic oligomers were detected and the decarboxylation phenomenon on top of the polymer surface was confirmed.

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supporting

confidence: 81%

Surface (XPS, SIMS) chemical investigation on poly(pyrrole-3-acetic acid) films electrosynthesized on Ti and TiAlV substrates for the development of new bioactive substrates

Giglio

Calvano

Losito

et al. 2005

Surf. Interface Anal.

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“…Covalent entrapment of the enzyme resulted in higher enzyme activity in the conducting polymer than that obtained previously for physical entrapment and was responsible for enhanced enzyme stability. Recently, Lowe and co-workers [63] further improved the biosensor by covalently attaching 3-carboxymethylpyrrole to the lysyl residues of glucose oxidase and copolymerized this moeity with unmodified pyrrole. An increased response to glucose due to the improved immobilized reactivity as compared to N-derivatized pyrrole-modified glucose oxidase and improved long term biosensor stability were realized.…”

Section: Immobilization Of Biocomponentsmentioning

confidence: 98%

Use of polymer films in amperometric biosensors

1995

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Different types of polymeric films implemented in various capacities in amperometric biosensor design are reviewed. Conducting and nonconducting polymer films and composite films are considered. Methods of film fabrication such as solvent casting, electropolymerization, and adsorption are presented. Film function, including prevention of interference and fouling problems, immobilization of the enzyme or active biocomponent, extension of linear range, or to address biocompatibility problems are described. The use of polymeric films as a medium to incorporate mediators, while an important function, is too vast for review here. The applications of polymer film-modified biosensors for selected analytical determinations are also presented. This review focuses upon the use of polymer films in amperometric biosensors and emphasizes innovative biosensor designs and unique applications, and omits traditional free-standing films unless they are coupled with new types of polymer films or used innovatively.

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Covalent Electropolymerisation of Glucose Oxidase in Polypyrrole

Cited by 2 publications

References 4 publications

Surface (XPS, SIMS) chemical investigation on poly(pyrrole-3-acetic acid) films electrosynthesized on Ti and TiAlV substrates for the development of new bioactive substrates

Surface (XPS, SIMS) chemical investigation on poly(pyrrole-3-acetic acid) films electrosynthesized on Ti and TiAlV substrates for the development of new bioactive substrates

Use of polymer films in amperometric biosensors

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