Covalent enzyme coupling on cellulose acetate membranes for glucose sensor development

Sternberg, R.; Bindra, Dilbir S.; Wilson, George S.; Thévenot, Daniel

doi:10.1021/ac00175a028

Cited by 73 publications

(31 citation statements)

References 40 publications

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“…This result suggests that benzoquinone inhibits the reduction of the ubiquinone pool, similarly to its action on the homologous reaction in the chloroplast. Inactivation of the NAD-(P)H:(quinone-acceptor) oxidoreductase is probably involved in both cases, due to the bifunctional linking of p-benzoquinone to amino acid residues (14) and in line with the strong sequence homology between enzymes reported by Fearnley et al (15). Fig.…”

supporting

confidence: 56%

Mitochondrial responses to intracellular pulses of photosynthetic oxygen

Lavergne

1989

Proc. Natl. Acad. Sci. U.S.A.

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When submitting anaerobic algal cells to a series of saturating flashes, transient absorption changes of mitochondrial origin were detected, showing the characteristic flash-number dependence of photosynthetic oxygen evolution. The faster kinetic event is the oxidation of heme a3 of the cytochrome-c oxidase, which reaches a maximum at =Z3.5 ms before again being reduced within 20 ms. The oxidation of cytochrome c involves an initial submillisecond lag, and its half-time is =3.3 ms. Another component, probably indicating oxidation of heme a, is seen around 607 nm, with a kinetic behavior similar to that of cytochrome c. The fast time scale of these reactions excludes long-range diffusion and supports a direct intracellular trapping of 02. It is estimated that, under appropriate conditions, the yield of this process is >30%. The linearity of these responses with respect to the amplitude of the oxygen pulse implies that a single turnover of the cytochrome oxidase is involved. These results suggest that the intracellular oxygen pathway may be of physiological importance in green algae. On the other hand, this technique seems promising both as an alternative to polarographic detection of photosynthetic oxygen and as a means of studying the cytochrome oxidase response in vivo to single-turnover oxygen pulses.In unicellular green algae, much cell space is occupied by the two types of energy-conserving organelles, chloroplast and mitochondrion. Most often a single large chloroplast is surrounded by a number of smaller mitochondria. When energetic aspects are considered, the biosphere may be viewed as a symbiotic association of both organelles (1), so that an algal cell then appears as a microscopic model of the biosphere. Encapsulation of chloroplast and mitochondrion within a cell optimizes the efficiency of their metabolic trade; this metabolism concerns chiefly carbohydrates among other interconnecting pathways (2, 3). Another component exchanged between the organelles is molecular oxygen. In our biosphere, the main route for the oxygen cycle, obviously, is through the atmosphere. Nevertheless, one may ask whether the direct intracellular pathway could occur with significant efficiency-that is, could an algal cell in an anaerobic environment respire by trapping molecular oxygen evolved from its own chloroplast? This question may have evolutionary as well as physiological bearings: should the answer be positive, the oxygen-using respiratory pathway could have evolved in a still anoxigenic atmosphere.The evidence reported in this paper shows that, indeed, a significant fraction of photosynthetic oxygen may be used by mitochondria before the oxygen diffuses out of the cell. By studying light-induced absorption changes in anaerobic suspensions of algae, signals arising from the oxidation of mitochondrial cytochrome-c oxidase and cytochrome c were seen. This finding provides a new method both for photosynthetic oxygen detection and for studying the cytochrome oxidase response to short oxygen pulses.MATERIALS AND M...

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supporting

confidence: 56%

Mitochondrial responses to intracellular pulses of photosynthetic oxygen

Lavergne

1989

Proc. Natl. Acad. Sci. U.S.A.

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“…These membranes were extensively explored in the 1970s because of their high perm-selectivity towards anions and the fact that they can be deposited by employing film casting or dip coating method [47]. Significant work has been conducted by Sternberg et al in which they developed various methods for immobilizing glucose oxidase enzyme on a cellulose acetate membrane [48]. Their work focused on the production of thin and stable membranes.…”

Section: Enzymatic Glucose Biosensor Membranesmentioning

confidence: 99%

Application of Semipermeable Membranes in Glucose Biosensing

Kulkarni

Slaughter

2016

Membranes

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Glucose biosensors have received significant attention in recent years due to the escalating mortality rate of diabetes mellitus. Although there is currently no cure for diabetes mellitus, individuals living with diabetes can lead a normal life by maintaining tight control of their blood glucose levels using glucose biosensors (e.g., glucometers). Current research in the field is focused on the optimization and improvement in the performance of glucose biosensors by employing a variety of glucose selective enzymes, mediators and semipermeable membranes to improve the electron transfer between the active center of the enzyme and the electrode substrate. Herein, we summarize the different semipermeable membranes used in the fabrication of the glucose biosensor, that result in improved biosensor sensitivity, selectivity, dynamic range, response time and stability.

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“…32 Cellulose acetate (CA) is one of the most important esters of cellulose that has frequently been used for a large number of applications. Sternberg et al 33 prepared a glucose sensor by immobilizing GOx on CA membranes. They initially activated and coupled the membrane with bovine serum albumin (BSA) in order to increase the number of functional groups of CA and to bring a proteic environment into the enzyme.…”

Section: Nanocellulose In Glucose Biosensor Applicationsmentioning

confidence: 99%

Cellulosic Nanomaterials for Sensing Applications

Abdi

Abdullah

Tahir

et al. 2014

Materials and Energy

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This chapter provides an overview of recent progress made in the area of cellulosebased nanocomposites for sensing application. Since nanocellulose shows high tensile strength and good water dispersibility and hydrophilic properties, it has been used to improve mechanical and dispersibility properties of the materials. Porous structure of the hydrolyzed polymeric cellulose minimizes barriers to mass transport between the analyte and immobilized indicator decreases the response time. The applications and new advances covered in this chapter are the use of nanocellulose in ion exchange membrane, glucose biosensor, actuators/strain sensor, and heavy metal ion sensor. This chapter will also include the application of cellulose nanocrystals with high surface area and negative charge in biosensor application and separation technologies. The high energy interaction between binding site on the cellulose nanocrystal film and the cationic species combined with the permselective properties make the cellulose film prime candidate for application in sensor devices and permselective membranes. IntroductionRecently, nanocelluloses as a biorenewable and biocompatible resource have received great attention to the scientists due to their unique structural and physical properties such as tensile, optical, and electrical properties. Because of these properties, they have been widely studied for application in materials, actuators/sensors, drug delivery system, and biomedical science. 1 Natural cellulose fibers are bundles of microfibrils which consist of crystalline and amorphous domains. Amorphous regions can be removed by hydrolysis process left behind the crystalline cellulose, which are called nanocrystals. Cellulose nanocrystals (CNCs) are long and straight crystals of cellulose with very large modulus elasticity and strength that can be used as biodegradable additives in nanocomposite materials. 2 Using cellulose nanosized as filler elements instead of other filler materials make the product efficient, biocompatible, cost-effective and, environmental friendly for fabrication of nanocomposite structure. 197Handbook of Green Materials Downloaded from www.worldscientific.com by KAINAN UNIVERSITY on 02/05/15. For personal use only.

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Covalent enzyme coupling on cellulose acetate membranes for glucose sensor development

Cited by 73 publications

References 40 publications

Mitochondrial responses to intracellular pulses of photosynthetic oxygen

Mitochondrial responses to intracellular pulses of photosynthetic oxygen

Application of Semipermeable Membranes in Glucose Biosensing

Cellulosic Nanomaterials for Sensing Applications

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