Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles

Uzun, Kerem; Çevik, Emre; Şenel, Mehmet; Sözeri, H.; Baykal, A.; Abasıyanık, M. Fatih; Toprak, Muhammet S.

doi:10.1007/s11051-010-9902-9

Cited by 73 publications

(26 citation statements)

References 45 publications

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“…This leads to a less favorable configuration of the active site [45,55,56]. Besides conformational changes, the decrease in K cat can also be ascribed to steric hindrances [13,[55][56][57]. …”

Section: Immobilized Invertase Kinetic Parameters In Continuous Flowmentioning

confidence: 99%

Sucrose Hydrolysis in a Bespoke Capillary Wall-Coated Microreactor

2017

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Abstract:Microscale technology has been increasingly used in chemical synthesis up to production scale, but in biocatalysis the implementation has been proceeding at a slower pace. In this work, the design of a low cost and versatile continuous flow enzyme microreactor is described that illustrates the potential of microfluidic reactors for both the development and characterization of biocatalytic processes. The core structure of the developed reactor consists of an array of capillaries with 450 µm of inner diameter with their inner surface functionalized with (3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde where Saccharomyces cerevisiae invertase was covalently bound. The production of invert sugar syrup through enzymatic sucrose hydrolysis was used as model system. Once the microreactor assembly reproducibility and the immobilized enzyme behavior were established, the evaluation of the immobilized enzyme kinetic parameters was carried out at flow rates ranging from 20.8 to 219.0 µL·min −1 and substrate concentrations within 2.0%-10.0% (w/v). Despite the impact of immobilization on the kinetic parameters, viz. K m(app) was increased two fold and K cat showed a 14-fold decrease when compared to solution phase invertase, the immobilization proved highly robust. For a mean residence time of 48.8 min, full conversion of 5.0% (w/v) sucrose was observed over 20 days.

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Section: Immobilized Invertase Kinetic Parameters In Continuous Flowmentioning

confidence: 99%

Sucrose Hydrolysis in a Bespoke Capillary Wall-Coated Microreactor

2017

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“…The particles were dried at room temperature (23 °C). FT-IR was used to check whether the reagent was bound to the surface of the particles, in a wavenumber range 4000-600 cm -1 [24,25]. TGA analysis was carried out with heating rate of 10 ºC/min in nitrogen until 900 ºC [26,27].…”

Section: Synthesis Of Adsorbent Materialsmentioning

confidence: 99%

Arsenate removal with 3-mercaptopropanoic acid-coated superparamagnetic iron oxide nanoparticles

Morillo

Uheida

Pérez

et al. 2015

Journal of Colloid and Interface Science

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In the present work, superparamagnetic iron oxide nanoparticles (SPION) surface-coated with 3-mercaptopropanoic acid (3-MPA) were prepared and their feasibility for the removal of arsenate from dilute aqueous solutions was demonstrated. The synthesized 3-MPA-coated SPION was characterized using transmission electron microscopy (TEM), thermogravimetric analysis (TGA) and Fourier transform infra-red spectrometry (FTIR). Separation efficiency of the coated nanoparticles and the equilibrium isotherm of arsenate adsorption were investigated. The obtained results reveal the arsenate adsorption to be highly pH-dependent, and the maximum adsorption was attained in less than 60 minutes. The resulting increase of 3-MPA-coated SPION adsorption capacity to twice the adsorption capacity of SPION alone under the same conditions is attributed to the increase of active adsorption sites. An adsorption reaction is proposed. On the other hand, efficient recovery of arsenate from the loaded nanoparticles was achieved using nitric acid (HNO 3 ) solution, which also provides a concentration over the original arsenate solution. HIGHLIGHTS• Feasibility of using SPION modified with 3MPA for the removal of As(V).• The adsorbent system results in an effective sorption for selective As(V) removal.• The adsorption capacity for As(V) is higher comparing with other adsorbent systems.• Adsorption mechanism has been proposed as the most suitable considering the results.

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“…Therefore, their surface functionalization and the investigation of their physicochemical properties are very important for their diverse applications. SPIONs can be synthesized by diverse methods such as co-precipitation (Uzun et al 2010), thermal decomposition (Sun and Zeng 2002), microemulsion (Solans et al 2005), hydrothermal synthesis (Hu et al 2007) and sonochemistry (Vijayakumar et al 2000). A suitable surface functionalization and choice of solvent are crucial in order to obtain sufficient repulsive interactions to prevent agglomeration of SPIONs.…”

Section: Introductionmentioning

confidence: 99%