No abstract

Polyvinylimidazole (PVI)-grafted iron oxide nanoparticles (PVIgMNP) were prepared by grafting of telomere of PVI on the iron oxide nanoparticles. Different metal ions (Cu(2+), Zn(2+), Cr(2+), Ni(2+)) ions were chelated on polyvinylimidazole-grafted iron oxide nanoparticles, and then the metal-chelated magnetic particles were used in the adsorption of invertase. The maximum invertase immobilization capacity of the PVIgMNP-Cu(2+) beads was observed to be 142.856 mg/g (invertase/PVIgMNP) at pH 5.0. The values of the maximum reaction rate (V max) and Michaelis-Menten constant (Km) were determined for the free and immobilized enzymes. The enzyme adsorption-desorption studies, pH effect on the adsorption efficiency, affinity of different metal ions, the kinetic parameters and storage stability of free and immobilized enzymes were evaluated.

In the present study, Eupergit ® C macroporous beads were functionalized with amino triazole. Cu2+ ions were chelated on the triazole modified Eupergit ® C (EC), and subsequently, the metal chelated beads were used in the adsorption of invertase. The maximum reaction rate (Vmax) and the Michaelis-Menten constant (Km) were determined for the free and the immobilized enzymes. The characteristics of immobilized invertase such as the temperature activity curve, thermal stability, operational stability, and storage stability were evaluated. The results indicated that triazole functionalized Eupergit ® C beads were well applicable for metal adsorption and enzyme immobilization.

No abstract