Covalent immobilization of redox protein via click chemistry and carbodiimide reaction: Direct electron transfer and biocatalysis

Microwave irradiated click reactions on silicon surfaces via derivertization of covalently grafted poly(PEGMA) brushes

“…Especially click chemistry is widely recognized in biomedical applications recently for its reaction in aqueous media and under room temperature. Either acetylene or azide moieties can be conveniently introduced onto solid surfaces [2][3][4][5][6][7][8][9][10][11][12][13][14] or combined with functional or biological molecules [4,6,[15][16][17][18][19][20][21]. Furthermore through click chemistry, a variety of probe molecules can be anchored onto solid surfaces for sensors and biochips.…”

Electrosynthesis of ERGO-NP Nanocomposite Films for Bioelectrocatalysis of Horseradish Peroxidase towards H2O2

“…[16][17][18] Thus, it is intriguing to modify conventional electrodes with favorable materials and immobilization method to entrap proteins on the electrode surface for better achievement of the DET process, toward practical biosensing devices. 19,20 A vast majority of recent research on electrochemical biosensors has focused on the incorporation of metal nanoparticles in the design of sensitive electrochemical sensors. 14 Recently, much effort has been focused on electrodeposition of nanoparticles due to their unique characteristics, such as the catalytic activities, optical, electronic, and magnetic properties that could not be observed in their bulk complement.…”

Copper(I)-Catalyzed Click Chemistry as a Tool for the Functionalization of Nanomaterials and the Preparation of Electrochemical (Bio)Sensors

“…In this context, click chemistry has been used as a versatile tool for different electrode materials functionalization using a variety of methodologies [13]. The characteristics and application of the recent configurations reported are summarized in Table 1 [10,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40]. In the following sections they are discussed according to the different functionalization strategies.…”

“…Structurally well-defined organic SAMs were formed on Au surface from a mixture of azidoundecanethiol (N 3 C 11 H 22 SH) and 1-decanethiol using 4-pentynoic acid as a bifunctional linker to immobilized hemoglobin (Hb) via click and carbodiimide reactions (Figure 2B). The Hb-functionalized Au electrode exhibited direct electron transfer, showing a pair of well-defined and quasi-reversible peaks owing to the Fe(III)/Fe(II) redox couple at about −0.210 V versus saturated calomel electrode (SCE), as well as excellent electrocatalytic activity toward O 2 and H 2 O 2 [14]. Furthermore, an electrochemical sensor for Cu(II) was constructed based on the activity of cuprous ion as catalyst in the CuAAC reaction involving a similar SAM configuration with mixed N 3 C 11 H 22 SH and octylthiol assembled on the gold electrode surface (Figure 2C).…”