Covalently bound DNA on naked iron oxide nanoparticles: Intelligent colloidal nano-vector for cell transfection

Porcine Dental Epithelial Cells Differentiated in a Cell Sheet Constructed by Magnetic Nanotechnology

“…This result was similar to previous research which reported the uptake of MNPs in different cell types [ 27 , 28 , 29 ]. The result obtained in the present study and the previous reports [ 27 , 28 , 29 ] suggest that MNPs can be used as carriers to deliver biologically active agents, such as growth factors and nanovectors, to odontogenic cells for control of histodifferentiation and organ generation in vivo [ 30 , 31 ]. We further investigated the cytotoxicity of MNPs in DECs and DMCs.…”

The surface reactivity of iron oxide nanoparticles as a potential hazard for aquatic environments: A study on Daphnia magna adults and embryos

“…Available zeta-potential values ( ζ ) of nanostructured iron oxides, at neutral pH, are generally close to zero 53 , explaining the very low colloidal stability of the suspensions of these nanomaterials. Conversely, naked SAMNs present a remarkable positive ζ value (+38.7 ± 8.7 mV, conductivity 0.00347 mS cm −1 ) 42 , responsible for the strong electrostatic repulsion between nanoparticles and conferring a unique stability to naked SAMNs as colloidal suspensions in water. In fact, in a manner similar to hydrous ferric oxides, the coordination of water molecules on SAMN surface leads to positively charged OH 2 +0.5 groups 54 .…”

“…Surface Active Maghemite Nanoparticles (SAMNs) stand out among iron oxide nanomaterials for their exceptional colloidal stability in the absence of any coating or surface modifier, leading to excellent cellular uptake 39 , low toxicity 40 , excellent contrast agent properties for magnetic resonance imaging (MRI) 41 , and their application as magnetic carriers for biomolecules 42 . These unusual characteristics can be usefully applied to deepen our knowledge on the effects of metal oxide nanomaterials on biological systems, without any interference by organic or inorganic shells.…”

Gene Therapy in Cancer Treatment: Why Go Nano?

“…To improve internalization and lysosomal release, functionalization with PEI has been used [184]. However, other authors proposed to transfer DNA coated by nude nanoparticles using an intelligent colloidal nanovector for transfection in equine peripheral blood-derived mesenchymal stem cells with success [185]. Moreover, the efficiency of DNA transfer increases using a magnetic field which delivers the nanoparticles through the cell compartments increasing the DNA delivery efficiency [186].…”