To achieve efficient weed control through cover cropping, the plant species chosen needs particular consideration. Combing different cover crop (CC) species in mixtures may increase the number of provided ecosystem services, including reliable suppression of weeds. We tested the weed suppression ability of single CC species and CC mixtures in a field trial during the autumn-to-winter growing season of 2016 and 2017. Anethum graveolens L. (dill), Raphanus sativus var. oleiformis Pers. (oilseed radish), Avena strigosa Schreb. (black oat), Carthamus tinctorius L. (safflower), Vicia sativa L. (vetch) and Phacelia tanacetifolia Benth. (phacelia) were sown in monocultures, as well as in mixtures with three or six species. Treatments with favorable establishment and above-average biomass yields tended to suppress weeds by showing lower weed dry matter and weed numbers. The highest weed control efficacy within the monocultures was reached in 2017 by black oat and oilseed radish with 72% and 83%, respectively. The mixture treatments reached a generally lower soil cover, aboveground dry matter and weed control efficacy (with an average of 57% in 2017). Even though mixtures were not as effective as the best performing single sown CCs, species combinations increased resilience against adverse weather conditions, an advantage to achieving efficient weed control over a long-term period. Therefore, species composition within mixtures is more relevant than the number of species included.Agronomy 2019, 9, 294 2 of 12 are left on the soil surface, they additionally act as a physical layer that small weed seedlings need to penetrate [13,14]. This slows down the development of the weed populations in spring after the main crop has already been sown [15]. Therefore, CCs are able to affect weed populations from their sowing date until a certain time after the subsequent main crop is established [16]. Naturally, the weed suppressive ability of a CC depends on several environmental influences that determine, e.g., the level and activity of allelochemicals [17], the speed of CC development and the build-up of biomass [18]. Under unfavorable conditions, a single sown CC might not be able to provide a sufficient level of weed suppression.Crop stands of single CC species are not able to buffer rapidly changing environmental conditions. Therefore, many studies have investigated the adaptability of mixtures [19][20][21]. Higher species diversity increases the likelihood that some of the species in a mixture are more productive, because they are better adapted to a certain set of environmental conditions (sampling effect) [22,23]. The CC species Vicia sativa L. and Phacelia tanacetifolia Benth. were not germinating well under high temperatures, whereas Guizotia abyssinica (L.f.) Cass. performed well [24]. Combinations of contrasting species in regard to environmental conditions, therefore, might provide resilience to weather conditions and provide stability in their service provision. The conditions that drive CC species performance are also d...