COVID-19 and risk management in a tissue bank

Paggiaro, André Oliveira; Conceição, Renata Oliveira; Bianchi, Mariana Parra; Gemperli, Rolf

doi:10.6061/clinics/2020/e2132

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…Therefore, tissue banks all over the world established strict preventive measures, or in some cases stopped collecting samples. On the other hand, at the moment, no documented reports have proved any COVID-19 infection following tissue transplantation [ 20 , 21 ]. We think that our results could represent a valid basis for further studies related to the possibility of bone donation in patients who suffered and healed from COVID-19.…”

Section: Discussionmentioning

confidence: 99%

Detection of SARS-CoV-2 in Cancellous Bone of Patients with COVID-19 Disease Undergoing Orthopedic Surgery: Laboratory Findings and Clinical Applications

Guazzoni

Castelli

Polizzi

et al. 2022

IJERPH

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An emerging issue for orthopedic surgeons is how to manage patients with active or previous COVID-19 disease, avoiding any major risks for the surgeons and the O.R. personnel. This monocentric prospective observational study aims to assess the prevalence of SARS-CoV-2 viral RT-PCR RNA in cancellous bone samples in patients with active or previous COVID-19 disease. We collected data about 30 consecutive patients from our institution from January 2021 to March 2021 with active or previous COVID-19 disease. The presence of SARS-CoV-2 in the samples was determined using two different PCR-based assays. Eighteen of the thirty patients included in the study had a positive nasopharyngeal swab at the time of surgery. Twelve patients had a negative nasopharyngeal swab with a mean days since negativization of 138 ± 104 days, ranging from 23 to 331 days. Mean days of positivity to the nasal swab were 17 ± 17. Twenty-nine out of thirty (96.7%) samples were negative for the presence of SARS-CoV-2 RNA. In one sample, low SARS-CoV-2 load (Cycle threshold (Ct) 36.6.) was detected but not confirmed using an additional confirmatory assay. The conducted study demonstrates the absence of the viral genome within the analyzed cancellous bone. We think that the use of personal protection equipment (PPE) to only protect from aerosol produced during surgery, both in active and recovered patients, is not strictly necessary. We think that the use of PPE should not be employed by surgeons and the O.R. personnel to protect themselves from aerosols produced from the respiratory tract. Moreover, we think that our results could represent a valid basis for further studies related to the possibility of bone donation in patients that suffered and recovered from COVID-19.

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Section: Discussionmentioning

confidence: 99%

Detection of SARS-CoV-2 in Cancellous Bone of Patients with COVID-19 Disease Undergoing Orthopedic Surgery: Laboratory Findings and Clinical Applications

Guazzoni

Castelli

Polizzi

et al. 2022

IJERPH

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“…Data concerning the risk of SARS-CoV-2 transmission after tissue transplantation are, to date, controversial [ 12 , 13 ]. Recently, several SARS-CoV-2 inactivating procedures have been suggested in the literature for application during human tissue processing, but unfortunately, the most promising of these techniques (i.e., gamma irradiation) is not applicable to valve and PVW processing due to tissue injury [ 14 ]. Thus, in order to implement screening techniques, donor selection parameters, and to better evaluate the theoretical risk of virus transmission within valve transplantation, we tested SARS-CoV-2 presence in PVW samples, preserving pulmonary valve leaflet structures, as well as in other cardiovascular tissues collected at our tissue biobank in the period of April–May 2020 from NPS- and/or BAL-negative tissue donors considered eligible for pulmonary valve (PV) transplant following actual guidelines.…”

Section: Introductionmentioning

confidence: 99%

Presence of SARS-CoV-2 Nucleoprotein in Cardiac Tissues of Donors with Negative COVID-19 Molecular Tests

et al. 2021

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The 2019 Coronavirus disease (COVID-19) outbreak had detrimental effects on essential medical services such as organ and tissue donation. Lombardy, one of the most active Italian regions in organ/tissue procurement, has been strongly affected by the COVID-19 pandemic. To date, data concerning the risk of SARS-CoV-2 transmission after tissue transplantation are controversial. Here, we aimed to evaluate the presence/absence of SARS-CoV-2 in different cardiac tissues eligible for transplantation obtained from Lombard donors. We used cardiovascular tissues from eight donors potentially suitable for pulmonary valve transplantation. All donor subjects involved in the study returned negative results for the SARS-CoV-2 RNA molecular tests (quantitative real-time reverse-transcription PCR, qRT-PCR, and chip-based digital PCR) in nasopharyngeal swabs (NPS) or bronchoalveolar lavage (BAL). None of the eight donors included in this study revealed the presence of the SARS-CoV-2 viral genome. However, evaluation of the protein content of pulmonary vein wall (PVW) tissue revealed variable levels of SARS-CoV-2 nucleoprotein signal in all donors. Our study demonstrated for the first time, to the best of our knowledge, that viral nucleoprotein but not viral RNA was present in the examined tissue bank specimens, suggesting the need for caution and in-depth investigations on implantable tissue specimens collected during the COVID-19 pandemic period.

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“…Only emergency or semi emergency cases were done and number of procedures per patient was limited as much as possible. Use of allograft completely stopped as allograft harvesting without proper testing of the donors and protection of the harvesting personnel could increase the risk of transmission of infection (8). Electric dermatome was not used inside the operating theater due to the risk of aerosol generation (9,10).…”

Section: Introductionmentioning

confidence: 99%

Burn Care in Kirtipur Hospital before and during COVID 19 Pandemic: Survey of Psychological Issues and Practice Behaviours of Burn Care Personnel

Nakarmi¹,

Basnet²,

Karki³

et al. 2021

J. Bangladesh Coll. Phys.

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Introduction: The COVID 19 pandemic has adversely impacted all aspects of health care throughout the world. Burn care in lower and middle income countries has suffered the most. We aimed to compare burn care at Kirtipur Hospital before and during the pandemic and explore psychological issues and practice behaviour among burn care worker (BCW). Methods: Retrospective data analysis of burn patients admitted during April to August of 2019 and 2020 was done. Internet based survey of BCW was done. Results: Burn admissions, demographics and characteristics did not change. Fewer surgeries were undertaken in 2020. Almost half of the BCW worked 12 hours shift or longer. Most were working on half pay. Nearly everyone was using hand sanitizers, hand washing and masks. Few had access to level II personal protective equipments (PPE) to see suspected patients; fewer had access to face shield, KN 95 masks and boot. Even gloves and caps were scarce. Many feared getting themselves or family members infected. Majority realized the need of clear hospital policy on how they would be managed when infected. Conclusion: Number, types, severity and mortality did not change. Number of surgeries decreased. Issues like pay cuts, longer working hours and lack of PPEs were reported by majority. J Bangladesh Coll Phys Surg 2021; 39: 241-248

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COVID-19 and risk management in a tissue bank

Cited by 4 publications

References 7 publications

Detection of SARS-CoV-2 in Cancellous Bone of Patients with COVID-19 Disease Undergoing Orthopedic Surgery: Laboratory Findings and Clinical Applications

Detection of SARS-CoV-2 in Cancellous Bone of Patients with COVID-19 Disease Undergoing Orthopedic Surgery: Laboratory Findings and Clinical Applications

Presence of SARS-CoV-2 Nucleoprotein in Cardiac Tissues of Donors with Negative COVID-19 Molecular Tests

Burn Care in Kirtipur Hospital before and during COVID 19 Pandemic: Survey of Psychological Issues and Practice Behaviours of Burn Care Personnel

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