CPMAS carbon-13 NMR study of lignin preparations from wheat straw transformed by five lignocellulose-degrading fungi

Almendros, G.; Martı́nez, Ángel T.; González, Aldo; Vila, Francisco Javier González; Fründ, R.; Lüdemann, H.‐D.

doi:10.1021/jf00019a043

Cited by 48 publications

(31 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3. The assignments of the most prominent NMR signals have been made in accordance with previous studies (Ludemann & Nimz, 1973;Maciel et al, 1985;Scalbert et al, 1986;Almendros et al, 1992). In NMR spectra for APPL extracted from inoculated wheat straw with NaOH, the signal at 31 p.p.m.…”

Section: Solid-state Cpmas 13c Nmrsupporting

confidence: 75%

See 1 more Smart Citation

Chemical characterization and spectroscopic analysis of the solubilization products from wheat straw produced by Streptomyces strains grown in solid-state fermentation

et al. 1997

View full text Add to dashboard Cite

The effects of two extraction procedures on the yield and properties of APPL (acid-precipitable polymeric lignin, or solubilized lignocellulose) produced by four streptomycetes during growth in solid-state fermentation were examined. When APPL was extracted with NaOH (0.1 M) rather than distilled water, y i e Ids increased threefold, with Streptomyces cha ttanoogensis exhibiting maximum solubilization levels [I63 mg product (g straw) '']. Alterations in the characteristics of APPL obtained during extraction with NaOH were detected using cross-polarization and magic-angle spinning (CPMAS) 13C NMR and IR spectroscopy and by GC-MS analysis after CuO oxidation, with the most significant changes detected in the cinnamic acid and lignin moieties. When APPL was extracted with NaOH, ester links between hemicellulose and lignin and between hemicellulose and cinnamic acid were cleaved, resulting in a decrease in the alkyl and carbonyl groups attached to lignin, enabling greater solubilization. Yields of APPL extracted with water were lower, but spectral characterization of this APPL suggested a possible role for actinomycete peroxidases and phenolic acid esterases in lignin solubilization. For industrial solubilization of lignocellulose, a possible role for the application of streptomycetes, or their enzymes, in alkali extraction is suggested as a means of increasing solubilization levels.Keywords : streptomycetes, acid-precipitable polymeric lignin, lignocellulose degradation INTRODUCTIONThere is considerable interest in the biological degradation of lignocellulose in the environment and its potential for industrial application. The biotechnological potential of waste biomass bioconversion has centred upon the biological and chemical treatment of lignocellulosic material to produce a range of products, including ethanol and sugars (Hartley et al., 1987). Also, the transformation of wheat straw during the growth of micro-organisms under solid-state fermentation conditions has been examined for the upgrading of straw to produce pulps and animal feed (for a review see Dart & Betts, 1991). The potential role of lignocellulosedegrading enzymes in the bleaching of pulp for paper has been investigated as an alternative or supplement to chemical bleaching techniques, which cause environmental problems through the release of chlorinecontaining effluents. Among the micro-organisms screened for the production of suitable enzymes, the actinomycetes are a group that have often been selected (Winter et al., 1991;Zhou et al., 1993).Actinomycetes produce a range of hydrolytic and oxidative enzymes which act co-operatively in degradation of the lignocellulose complex during primary growth (Ball et al., 1990). The mechanisms involved in this degradation process have not yet been fully elucidated; however, the main role attributed to these micro- organisms is solubilization rather than depolymerization and mineralization of lignin (McCarthy, 1987). The main product of solubilization of wheat straw and other lignocellulosic residues by act...

show abstract

Section: Solid-state Cpmas 13c Nmrsupporting

confidence: 75%

“…protein, aliphatic acids, cinnamic acids) (Almendros et al, 1992 ;Gamble et al, 1994;Reeves & Schmidt, 1994). The variation in this signal must be confirmed by specific determination of the above mentioned compounds in APPL from uninoculated and inoculated wheat straw.…”

Section: Solid-state Cpmas 13c Nmrmentioning

confidence: 94%

Chemical characterization and spectroscopic analysis of the solubilization products from wheat straw produced by Streptomyces strains grown in solid-state fermentation

et al. 1997

View full text Add to dashboard Cite

show abstract

“…Assignment of the principal signals of lignin in the region of the spectrum between 110 and 160 ppm (Bardet et al, 1985;Manders, 1987;Almendros et al, 1992) (Table 1) suggested that the nature of the lignin deposited in the early lignifying tissues near the intercalary meristem differed from that of the fully lignified tissues of the top of the internode. This suggests that the activities of monolignolsynthesizing enzymes are not uniform as the internode extends.…”

Section: Lignin Analysis (Cp-mas 13c-nmr and Ftlr Spectroscopy)mentioning

confidence: 99%

Study of Lignification by Noninvasive Techniques in Growing Maize Internodes (An Investigation by Fourier Transform Infrared Cross-Polarization-Magic Angle Spinning 13C-Nuclear Magnetic Resonance Spectroscopy and Immunocytochemical Transmission Electron Microscopy)

1997

View full text Add to dashboard Cite

Noninvasive techniques were used for the study in situ of lignification in the maturing cell walls of the maize (Zea mays L.) stem. Within the longitudinal axis of a developing internode all of the stages of lignification can be found. The synthesis of the three types of lignins, p-hydroxyphenylpropane (H), guaiacyl (C), and syringyl (S), was investigated in situ by cross-polarization-magic angle spinning '3C-solid-state nuclear magnetic resonance, Fourier transform infrared spectroscopy, and immunocytochemical electron microscopy. The first lignin appearing in the parenchyma is of the G-type, preceeding the incorporation of S nuclei in the later stages. How- Lignin is a complex phenyl propanoid polymer, which is integrated into the wall framework of vascular plant cells. This polymer is complex and heterogeneous with respect to the relative proportions of its three constituting monolignol units (Higuchi, 1990). Variations in lignin composition occur within plant species, tissues, cell types, and also according to developmental stage (Campbell and Sederoff, 1996). The ultrastructural heterogeneity of lignin in secondary walls may be more complex than has so far been demonstrated, because it may be influenced by various factors such as the nature of phenoxy radicals, their steady-

show abstract

“…The resulting normalized 13 C CP/MAS NMR spectra are depicted in Fig. 1 (Almendros et al 1992;Gilardi et al 1995;Sun et al 2005;Zimbardi et al 1999). As can be seen, all of the acquired spectra were comprised mainly of chemical resonance values analogous to the carbohydrate region (60 to 100 ppm) and aromatic region (100 to 162 ppm).…”

Section: Structure Decomposition Analysis Of Biopretreated Corn Stovermentioning

confidence: 99%

Medium-Temperature Pyrolysis of Corn Stover Improved by Biopretreatment with White-rot Fungi

Yang¹,

Laskar²,

Ma³

et al. 2013

BioResources

View full text Add to dashboard Cite

This work investigated the ability of biopretreatment with different whiterot fungi to improve the medium-temperature pyrolysis of biomass. It was found that biopretreatment can significantly increase the production of phenols and glucopyranoside up to 2.82 and 2.94 fold, respectively. Biopretreatment can also decrease the content of carbon dioxide, propanol, and propanone, making the pyrolysis more efficient and product-oriented. Moreover, distinct bio-deconstruction mechanisms can result in different pyrolysis products. By deconstructing cellulose and modifying lignin with a minimum of demethoxyation, white-rot fungus Irpex lacteus CD2 can improve the production of acetaldehyde (up to 6.72%) and methoxyl substitutes such as dimethoxyphenyl (up to 21.59 folds). By decomposing carbohydrates, carbonxyl, and methoxyl groups, white-rot fungi Pleurotus ostreatus BP2 and Echinodontium taxodii 2538 can increase the production of D-allose (up to 3.09%) and formic acid (up to 6.98%), while decreasing the methoxyl substitutes such as 2-methoxy-4-vinylphenol (up to 70.08%).

show abstract

CPMAS carbon-13 NMR study of lignin preparations from wheat straw transformed by five lignocellulose-degrading fungi

Cited by 48 publications

References 27 publications

Chemical characterization and spectroscopic analysis of the solubilization products from wheat straw produced by Streptomyces strains grown in solid-state fermentation

Chemical characterization and spectroscopic analysis of the solubilization products from wheat straw produced by Streptomyces strains grown in solid-state fermentation

Study of Lignification by Noninvasive Techniques in Growing Maize Internodes (An Investigation by Fourier Transform Infrared Cross-Polarization-Magic Angle Spinning 13C-Nuclear Magnetic Resonance Spectroscopy and Immunocytochemical Transmission Electron Microscopy)

Medium-Temperature Pyrolysis of Corn Stover Improved by Biopretreatment with White-rot Fungi

Contact Info

Product

Resources

About