Craniofacial chondrogenesis in organoids from human stem cell-derived neural crest cells

Foltz, Lauren; Avabhrath, Nagashree; Lanchy, Jean-Marc; Levy, Tyler; Possemato, Anthony; Ariss, Majd; Peterson, Bradley; Grimes, Mark

doi:10.1016/j.isci.2024.109585

iScience

2024

DOI: 10.1016/j.isci.2024.109585

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Craniofacial chondrogenesis in organoids from human stem cell-derived neural crest cells

Lauren Foltz,

Nagashree Avabhrath,

Jean-Marc Lanchy

et al.

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Cited by 3 publications

References 157 publications

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Research Progress in Hydrogels for Cartilage Organoids

Li,

Sheng,

et al. 2024

Adv Healthcare Materials

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The repair and regeneration of cartilage has always been a hot topic in medical research. Cartilage organoids (CORGs) are special cartilage tissue created using tissue engineering techniques outside the body. These engineered organoids tissues provide models that simulate the complex biological functions of cartilage, opening new possibilities for cartilage regenerative medicine and treatment strategies. However, it is crucial to establish suitable matrix scaffolds for the cultivation of CORGs. In recent years, utilizing hydrogel to culture stem cells and induce their differentiation into chondrocytes has emerged as a promising method for the in vitro construction of CORGs. In this review, we summarize the methods for establishing CORGs and provide an overview of the advantages and limitations of using matrigel in the cultivation of such organoids. Furthermore, we discuss the importance of cartilage tissue extracellular matrix (ECM) and alternative hydrogel substitutes for Matrigel, such as alginate, peptides, silk fibroin, and DNA derivatives, and outline the pros and cons of using these hydrogels for the cultivation of CORGs. Finally, we discuss the challenges and future directions in hydrogel research for CORGs. It is our hope that this article provides valuable references for the design and development of hydrogels for CORGs.This article is protected by copyright. All rights reserved

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Research Progress in Hydrogels for Cartilage Organoids

Li,

Sheng,

et al. 2024

Adv Healthcare Materials

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Protocol for generating human craniofacial cartilage organoids from stem-cell-derived neural crest cells

Avabhrath,

Foltz,

Grimes

2025

STAR Protocols

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Evaluation of Postoperative Outcomes Following Early and Late Palate Repair: A Preclinical Study

Aellos,

Verma,

et al. 2024

Journal of Craniofacial Surgery

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Objective: To quantitatively assess the impact of early versus late surgical intervention on midfacial growth using a mouse model. Methods: A full-thickness mucoperiosteal flap surgery was performed on newborn (P17) mice and on neonatal (P30) mice. High-resolution micro–computed tomographic imaging coupled with histomorphometric analyses was used to assess craniomaxillofacial growth. Histology and immunohistochemical analyses were used to assess cellular and molecular responses postsurgery. Results: Early surgical intervention at P17 resulted in significant midfacial growth arrest, with pronounced maxillary hypoplasia. Histomorphometric analyses revealed significant (P < 0.05) growth disruptions in the mid-palatal suture complex, including premature removal of the cartilaginous growth plate and its replacement by bone. In the suture itself, cell proliferation was significantly reduced (P < 0.05) compared with controls. The same surgical intervention performed in mice at P30 did not lead to significant midfacial growth arrest. Conclusions: Early surgical intervention in a mouse model mirrors the adverse growth outcomes in children undergoing early cleft repair. Molecular and cellular observations accompanying this midfacial growth arrest may inform therapeutic strategies to mitigate midfacial growth disturbances in patients and highlight the need for refined surgical techniques to minimize adverse growth outcomes.

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Craniofacial chondrogenesis in organoids from human stem cell-derived neural crest cells

Cited by 3 publications

References 157 publications

Research Progress in Hydrogels for Cartilage Organoids

Research Progress in Hydrogels for Cartilage Organoids

Protocol for generating human craniofacial cartilage organoids from stem-cell-derived neural crest cells

Evaluation of Postoperative Outcomes Following Early and Late Palate Repair: A Preclinical Study

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