Cre-expressing neurons in the cortical white matter of Ntsr1-Cre GN220 mice

Sundberg, Sofie; Granseth, Björn

doi:10.1016/j.neulet.2018.03.053

Cited by 7 publications

(9 citation statements)

References 23 publications

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“…4,12,23 We were intrigued by the idea that non-auditory inputs could naturally play the role of our laser pulse by recruiting L6 CT neurons to spike shortly before the onset of upcoming acoustic events. We were particularly struck by the nearly perfect coexpression of the forkhead box protein P2 (FoxP2) in cortical Ntsr1-Cre+ neurons, 24,25 a protein that-in mice-is highly enriched in motor control nuclei and distributed brain regions that integrate sensory inputs with motor-related signals. 26 The selective expression of FoxP2 in Ntsr1-Cre neurons reported in other cortical areas led us to question whether the activity of L6 CT neurons might be best understood in the context of sensorimotor integration.…”

Section: Resultsmentioning

confidence: 99%

Auditory Corticothalamic Neurons Are Recruited by Motor Preparatory Inputs

et al. 2021

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Section: Resultsmentioning

confidence: 99%

Auditory Corticothalamic Neurons Are Recruited by Motor Preparatory Inputs

et al. 2021

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“…complete and specific overlap of L6 CT and Ntsr1+ neurons (Bortone et al, 2014;Kim et al, 2015;Sundberg et al, 2018).…”

Section: Functional Excitatory Input To L6bmentioning

confidence: 99%

Layer 6b Is Driven by Intracortical Long-Range Projection Neurons

et al. 2020

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“…In these neurons, ACh induces a slowly 70 desensitizing inward current in L6 PCs of prefrontal cortex through activation of α4β2 subunit 71 containing synaptic nicotinic acetylcholine receptors (nAChRs) (Alves et al, 2010;Bailey et 72 al., 2012;Hay et al, 2016;Kassam et al, 2008;Poorthuis et al, 2013). However, there are 73 very few studies focusing on the effects of muscarinic acetylcholine receptors (mAChRs) in 74 L6A neurons (Sundberg et al, 2018;Tian et al, 2014). Here, using single and paired patch-75 clamp recordings with simultaneous biocytin-filling, we investigated both muscarinic and 76 nicotinic modulation of morphologically identified excitatory neurons and their synaptic 77 connections in layer 6A of rat primary somatosensory barrel cortex.…”

Section: Introductionmentioning

confidence: 99%

“…The differences in passive and active electrophysiological properties found here 208 are in accordance with previous studies(Kumar and Ohana, 2008;Tian et al, 2014). 209Furthermore, the nuclear transcription factor Fork-head box protein P2 (FoxP2) is co-210 expressed with the neurotensin receptor 1 (NtsR1) gene, a molecular marker for CT L6A 211PCs in mice(Sundberg et al, 2018; Tasic et al, 2016). To identify the expression of FoxP2 212…”

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confidence: 99%

Cell Type-Specific Modulation of Layer 6A Excitatory Microcircuits by Acetylcholine in Rat Barrel Cortex

Yang¹,

Günter²,

Qi³

et al. 2019

Preprint

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22Acetylcholine (ACh) is known to regulate cortical activity during different behavioral states, e.g. 23 wakefulness and attention. Here we show a differential expression of muscarinic ACh receptors 24 (mAChRs) and nicotinic AChRs (nAChRs) in different layer 6A (L6A) pyramidal cell (PC) types 25 of somatosensory cortex. At low concentrations, ACh induced a persistent hyperpolarization in 26 corticocortical (CC) but a depolarization in corticothalamic (CT) L6A PCs via M4 and M1 mAChRs, 27 respectively. At ~1 mM ACh depolarized exclusively CT PCs via α4β2 subunit-containing nAChRs 28 without affecting CC PCs. Miniature EPSC frequency in CC PCs was decreased by ACh but 29 increased in CT PCs. In synaptic connections with a presynaptic CC PC, glutamate release was 30 suppressed via M4 mAChR activation but enhanced by nAChRs via α4β2 nAChRs when the 31 presynaptic neuron was a CT PC. Thus, in layer 6A the interaction of mAChRs and nAChRs results 32 in an altered excitability and synaptic release, effectively strengthening corticothalamic output 33 while weakening corticocortical synaptic signaling. 34 35 Keywords: barrel cortex, layer 6, pyramidal cells, acetylcholine, muscarinic receptors, nicotinic 36 receptors, corticocortical, corticothalamic 37Acetylcholine (ACh) has been shown to play a major role in memory processing, arousal, attention 40 and sensory signaling 1-7 . It has been demonstrated that the ACh concentration in the cerebrospinal 41 fluid increases during wakefulness and sustained attention 8,9 . In the neocortex release of ACh occurs 42 predominately via afferents originating from cholinergic neurons in the nucleus basalis of Meynert 43 of the basal forebrain 10-12 ; their terminals are densely distributed throughout all neocortical layers 13-44 15 . A classical view is that ACh invariably increases the excitability of excitatory neurons in 45 neocortex 16-20. However, a persistent hyperpolarization in layer 4 (L4) excitatory neurons was 46 found in somatosensory cortex 21,22 . This layer-specific cholinergic modulation may contribute to 47 improving the cortical signal-to-noise ratio [23][24][25] . 48Although extensive studies have been conducted on the cholinergic modulation of neocortical 49 excitatory neurons, the action of ACh on the layer 6 (L6) microcircuitry has not been systematically 50 investigated. Two main pyramidal cell (PC) classes exist in cortical layer 6, namely corticothalamic 51 (CT) and corticocortical (CC) PCs. These two neuron types differ in their axonal projection patterns, 52 dendritic morphological features, electrophysiological properties and expression of molecular 53 markers 26-30 . CC PCs have no subcortical target and send intracortical projections mainly within the 54 infra-granular layers 28 ; CT PCs, in contrast, have few axons distributed in cortex and send 55 projections directly back to the thalamus thereby contributing to a feedback control of sensory 56 input 31-35 . The question how the function of these two classes of L6 PCs is modulated by ACh has ...

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Cre-expressing neurons in the cortical white matter of Ntsr1-Cre GN220 mice

Cited by 7 publications

References 23 publications

Auditory Corticothalamic Neurons Are Recruited by Motor Preparatory Inputs

Auditory Corticothalamic Neurons Are Recruited by Motor Preparatory Inputs

Layer 6b Is Driven by Intracortical Long-Range Projection Neurons

Cell Type-Specific Modulation of Layer 6A Excitatory Microcircuits by Acetylcholine in Rat Barrel Cortex

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