Creatine kinase and its isoenzymes in dog sera

ORIGINAL RESEARCH: Electrophoretic fractionation of creatine kinase isoenzymes and macroenzymes in clinically healthy dogs and cats and preliminary evaluation in central neurologic disease

“…This decrease, however, accounts for <10% of native CK activity. Low total CK activity in our canine group was similar to that reported in previous studies, 8,11 and discrepancies between the serum CK activities in healthy animals, especially cats, in this study and other studies 18,19 may result from differences in analytical methods rather than storage artifacts. Similarly, analysis of interassay imprecision for isoenzyme fractions in pooled serum samples did not reveal a common trend (neither an increase nor a decrease of percentages) associated with prior storage at −20°C.…”

“…The limited information about CK isoenzymes in dogs is focused on CK‐MM and CK‐MB 5–13 . Only one study reported serum CK‐BB activity in dogs with neurologic disease 12 .…”

“…In healthy dogs, CK‐MM was the most abundant electrophoretic fraction, but significant CK‐BB activity was also detectable. Other studies have reported similar activities of CK‐MM or CK‐BB, 9,10 but 1 study demonstrated greater activities of CK‐BB than CK‐MM 8 . The most likely explanation for this discrepancy is that the technique used in the latter study was based on electrophoretic methods that did not differentiate macroenzymes, which are known to interfere with identification of isoenzymes 22,23 .…”

“…However, CK‐BB could have deteriorated during storage conditions and may have been subsequently detectable in those samples in which CK‐BB activity was markedly increased before freezing. Another possible explanation is individual variability, which was detected both in this and in previous studies 8–10 . Individual characteristics of the sampled populations, possibly influenced by physiologic conditions, such as exercise and diet, could have caused discrepant results.…”

Catalytic Enzyme Activity Concentration in Plasma of Man, Sheep, Dog, Cat, Rabbit, Guinea Pig, Rat and Mouse. Approach to a Quantitative Diagnostic Enzymology. I. Communication

“…Considering further the up to 60-fold higher catalytic activities of creatine kinase in platelets of small laboratory animals and dogs compared with man (12,13), it is highly probable that the high values for creatine kinase in serum of these animals originate from platelets during clotting (61,64,65). A plateletderived acid phosphatase is seen in serum but not in plasma (66).…”