Creatine kinase isoenzymes in serum from cord blood and the blood of healthy full-term infants during the first three postnatal days.

Abstract

Isoenzymes of creatine kinase (ATP:creatine phosphotransferase; EC 2.7.3.2; CK) were measured by electrophoresis in serum from cord blood and skin-puncture blood taken from 45 healthy full-term infants during the first three postnatal days. Mean total CK activities (in U/L at 30 degrees C) were 185 in cord samples, 536 in samples taken between 5--8 h postnatally, 494 between 24--33 h, and 288 in the 72-100 h samples. Values for all three isoenzymes increased to a peak over this period, with the highest values generally being found in the samples taken 5--33 h after birth; the subsequent decline was most rapid for CK-BB. Serum CK isoenzymes in cord samples and those taken at 72--100 h in the 11 babies delivered by cesarian section did not differ significantly from those of babies delivered vaginally. However the postnatal increases in total CK, CK-MM, and CK-MB (but not in CK-BB) were significantly greater in those patients born by vaginal delivery. The reasons for the increases in CK isoenzymes after birth are not clear, but our results and reported studies on the ontogeny of CK suggest that CK-MB cannot be regarded as a "cardiac-specific" isoenzyme in the neonatal period.

Is the neonatal creatine phosphokinase level a reliable marker for fetal hypoxia?

“…17 A previous study reported that CPK levels were higher in patients delivered vaginally than in those delivered by cesarean. 18,19 Although there was no significant difference, we found higher levels of CPK in neonates delivered vaginally compared to those delivered by elective cesarean. This suggests that physiological uterine contractions during normal labor induced mild hypoxic stress to the fetus and may have increased CPK levels in the neonate.…”

Myocardial Ischaemia in Aphyxia Neonatorum

“…ECG's and enzyme levels were derived from a population of healthy babies described previously (8,9). The 43 babies from this population with adequate enzyme assays and ECG recordings available were included in this study.…”

“…The senim was separated within 2 hours and stored at 4°C for up to 3 days before analysis. Serum CK isoenzymes were assayed as previously described (9).…”

Association of Elevated Umbilical Cord Blood Creatine Kinase and Myoglobin Levels with the Presence of Cocaine Metabolites in Maternal Urine

“…Previous work by Jedeikin and associates demonstrated that CK in cord blood samples from vaginally delivered infants were not elevated compared to infants delivered by caesarean section; thus, we presumed route of delivery was not a confounding bias. 17 Jedeikin further found that CK levels increased after delivery, excluding a maternal source of CK from placental transit. Additionally, in the present study, the use of maternal urine testing presumed that negative tests were associated with no exposure in the newborn.…”