Creating a model of diseased artery damage and failure from healthy porcine aorta

Abstract

Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/) eprints@whiterose.ac.uk https://eprints.whiterose.ac.uk/ Reuse Unless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request.Creating a model of diseased artery damage and failure from healthy porcine aorta AbstractLarge quantities of diseased tissue are required in the research and development of new generations of medical devices, for example for use in physical testing. However, these are difficult to obtain. In contrast, porcine arteries are readily available as they are regarded as waste. Therefore, reliable means of creating from porcine tissue physical models of diseased human tissue that emulate well the associated mechanical changes would be valuable. To this end, we studied the effect on mechanical response of treating porcine thoracic aorta with collagenase, elastase and glutaraldehyde. The alterations in mechanical and failure properties were assessed via uniaxial tension testing. A constitutive model composed of the Gasser-Ogden-Holzapfel model, for elastic response, and a continuum damage model, for the failure, was also employed to provide a further basis for comparison [1,2]. For the concentrations used here it was found that: collagenase treated samples showed decreased fracture stress in the axial direction only; elastase treated samples showed increased fracture stress in the circumferential direction only; and glutaraldehyde samples showed no change in either direction. With respect to the proposed constitutive model, both collagenase and elastase had a strong effect on the fibre-related terms. The model more closely captured the tissue response in the circumferential direction, due to the smoother and sharper transition from damage initiation to complete failure in this direction. Finally, comparison of the results with those of tensile tests on diseased tissues suggests these treatments indeed provide a basis for creation of physical models of diseased arteries.

Measurement of Friction-induced Changes in Pig Aorta Fibre Organization by Non-invasive Imaging as a Model for Detecting the Tissue Response to Endovascular Catheters

“…previously reported that collagenase and elastase treatment of porcine aorta alters the uniaxial tension response [19]. Therefore this may provide insight into the change observed in that study.…”

“…To achieve this we used an experimental system, developed previously by the authors [19] to determine methodologies for applying loads reproducibly and analysing the response of the tissue to normal and frictional loading through non-invasive imaging.…”

“…Noble et al performed uniaxial tensile testing of collagenase and elastase treated samples to create a model of diseased aorta [19]. They report weakening of collagenase treated samples in the axial direction.…”

“…However this may be due to the loss of remaining structure such that no clear fibre angle could be detected. Noble et al previously reported that collagenase and elastase treatment of porcine aorta alters the uniaxial tension response [19].…”

In Silico Performance of a Recellularized Tissue-Engineered Transcatheter Aortic Valve

“…1 available in the Supplemental Materials on the ASME Digital Collection) [21]. In the latter technique, a standard fitting procedure was performed, [22,23] and these parameters are then placed into a finite element simulation of the equibiaxial loading performed in the biaxial testing. Predicted PK1 stress components from the FE model (P FE ii Þ and the analytical solution (P calc ii ) are then utilized to find a correction vector at each time point t according to…”