Creating Genetically Modified Marmosets

Sasaki, Erika

doi:10.1016/b978-0-12-811829-0.00020-0

Cited by 6 publications

(6 citation statements)

References 139 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Marmoset ovulation is usually defined by serum progesterone levels exceeding 10 ng/ml [15]. The average length of the ovarian cycle is 28.63 ± 1.01 days, including 8-13 days in the follicular phase [16,17]. Based on our studies, the marmoset follicular phase lasts 10.7 ± 1.3 days.…”

Section: Development Of Reproductive Biological and Assisted Reproducmentioning

confidence: 65%

Development of genetically modified nonhuman primates toward models for translational research

Sasaki

2019

Translational and Regulatory Sciences

Self Cite

View full text Add to dashboard Cite

Since the genetic, metabolic, and physiological characteristics of nonhuman primates (NHPs) are very similar to those of humans, NHPs serve as excellent animal models for biomedical research. Recent advances in genetic engineering and assisted reproductive technologies related to NHPs have enabled the development of genetically modified NHP models for human disease related studies. The common marmoset (Callithrix jacchus) is a useful laboratory animal model that is suitable for generating genetically engineered models due to its unique reproductive characteristics, such as prolific breeding, relatively short gestation period and small body size. Genetically modified marmoset models may be used as critical intermediaries to accelerate the progression of various conceptual technologies and therapeutics from the basic research stage to the clinical application stage. The last decade has witnessed the growth of genetic engineering technologies pertaining to NHPs as well as new gene modification technologies, such as gene editing. Such technological advances have enabled the development of genetically engineered NHP disease models for translational research.

show abstract

Section: Development Of Reproductive Biological and Assisted Reproducmentioning

confidence: 65%

Development of genetically modified nonhuman primates toward models for translational research

Sasaki

2019

Translational and Regulatory Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

“…Among New World monkeys there is growing interest in common marmosets ( Callithrix jacchus ), small simians (adults ~ 300 g) which have a published whole draft genome [ 27 ] and newer annotated reference assemblies. Marmosets are notable for litters of 2–4 offspring that are hematopoietic chimeras, as outgroups for catarrhine evolutionary studies, and for their potential in genetic engineering studies [ 28 , 29 ]. Regarding prosimians an ongoing initiative is investigating the translational potential of mouse lemurs ( Microcebus spp.…”

Section: Nonhuman Primate Models Of Diseasementioning

confidence: 99%

Nonhuman primate genetic models for the study of rare diseases

Vallender

Hotchkiss

Lewis

et al. 2023

Orphanet J Rare Dis

View full text Add to dashboard Cite

Pre-clinical research and development relies heavily upon translationally valid models of disease. A major difficulty in understanding the biology of, and developing treatments for, rare disease is the lack of animal models. It is important that these models not only recapitulate the presentation of the disease in humans, but also that they share functionally equivalent underlying genetic causes. Nonhuman primates share physiological, anatomical, and behavioral similarities with humans resulting from close evolutionary relationships and high genetic homology. As the post-genomic era develops and next generation sequencing allows for the resequencing and screening of large populations of research animals, naturally occurring genetic variation in nonhuman primates with clinically relevant phenotypes is regularly emerging. Here we review nonhuman primate models of multiple rare genetic diseases with a focus on the similarities and differences in manifestation and etiologies across species. We discuss how these models are being developed and how they can offer new tools and opportunities for researchers interested in exploring novel therapeutics for these and other genetic diseases. Modeling human genetic diseases in translationally relevant nonhuman primates presents new prospects for development of therapeutics and a better understanding of rare diseases. The post-genomic era offers the opportunity for the discovery and further development of more models like those discussed here.

show abstract

“…While it has been shown that iPSCs can allograft into the putamen of cynomolgus monkeys without immunosuppression [ 49 ], cjESC-derived cells were only tested using immunosuppressive agents such as tacrolimus [ 50 ]. However, it was reported that marmoset ESCs do enable allograft or autograft transplantations in the absence of immunosuppressive agents, presumably in other marmosets, and thus may facilitate a more precise assessment of the safety and efficacy of stem cell transplantation [ 51 ]. In summary, cjESCs provide important research tools for basic and applied research that could not be carried out with human ESCs (hESC) due to ethical and moral considerations.…”

Section: Marmoset Embryonic Stem Cellsmentioning

confidence: 99%

Utility of Common Marmoset (Callithrix jacchus) Embryonic Stem Cells in Liver Disease Modeling, Tissue Engineering and Drug Metabolism

Aravalli

Steer

2020

Genes

View full text Add to dashboard Cite

The incidence of liver disease is increasing significantly worldwide and, as a result, there is a pressing need to develop new technologies and applications for end-stage liver diseases. For many of them, orthotopic liver transplantation is the only viable therapeutic option. Stem cells that are capable of differentiating into all liver cell types and could closely mimic human liver disease are extremely valuable for disease modeling, tissue regeneration and repair, and for drug metabolism studies to develop novel therapeutic treatments. Despite the extensive research efforts, positive results from rodent models have not translated meaningfully into realistic preclinical models and therapies. The common marmoset Callithrix jacchus has emerged as a viable non-human primate model to study various human diseases because of its distinct features and close physiologic, genetic and metabolic similarities to humans. C. jacchus embryonic stem cells (cjESC) and recently generated cjESC-derived hepatocyte-like cells (cjESC-HLCs) could fill the gaps in disease modeling, liver regeneration and metabolic studies. They are extremely useful for cell therapy to regenerate and repair damaged liver tissues in vivo as they could efficiently engraft into the liver parenchyma. For in vitro studies, they would be advantageous for drug design and metabolism in developing novel drugs and cell-based therapies. Specifically, they express both phase I and II metabolic enzymes that share similar substrate specificities, inhibition and induction characteristics, and drug metabolism as their human counterparts. In addition, cjESCs and cjESC-HLCs are advantageous for investigations on emerging research areas, including blastocyst complementation to generate entire livers, and bioengineering of discarded livers to regenerate whole livers for transplantation.

show abstract

Creating Genetically Modified Marmosets

Cited by 6 publications

References 139 publications

Development of genetically modified nonhuman primates toward models for translational research

Development of genetically modified nonhuman primates toward models for translational research

Nonhuman primate genetic models for the study of rare diseases

Utility of Common Marmoset (Callithrix jacchus) Embryonic Stem Cells in Liver Disease Modeling, Tissue Engineering and Drug Metabolism

Contact Info

Product

Resources

About