Creating sub-50 nm nanofluidic junctions in a PDMS microchip via self-assembly process of colloidal silica beads for electrokinetic concentration of biomolecules

Syed, Abeer; Mangano, Lauren M.; Mao, Pan; Han, Jongyoon; Song, Yong-Ak

doi:10.1039/c4lc00895b

Cited by 53 publications

(43 citation statements)

References 27 publications

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“…Accordingly, Syed et al. presented an electrokinetic preconcentration system in which a sub‐50 nm cation‐selective nanoporous membrane was fabricated through the self‐assembly of colloidal silica beads. The ability of the nanoporous membrane to induce an ICP concentration effect was demonstrated experimentally using a DNA sample (Fig.…”

Section: Existing Sample Preconcentration Methodsmentioning

confidence: 99%

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Sample preconcentration from dilute solutions on micro/nanofluidic platforms: A review

Hou

Chiu

et al. 2017

Electrophoresis

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Biochemical detection plays a critical role in many analytical fields. For example, blood samples include many proteins with relevance to disease diagnosis and therapeutic monitoring. Foods and beverages contain a large number of chemicals and compounds which must be quantified and characterized to ensure their compliance with safety standards. Detecting trace amounts of contaminants in ambient air or water samples is essential in monitoring the environment and protecting human health. Therefore, effective techniques for performing the rapid and reliable detection of targeted analytes are required. Compared to conventional macroscale devices, microfluidic systems have many advantages, including a greater sensitivity, a faster response time, a reduced sample and reagent consumption, and a greater portability. Accordingly, many microfluidic systems for sample detection have been proposed in recent years. The performance of such devices relies on the target analyte being present in a sufficient concentration to enable its detection. In many biomedical, food testing and environmental applications, the detection limit was restricted. Thus, the sample must first be concentrated before the detection process is carried out. Accordingly, this review provides a comprehensive review of recent advances for sample preconcentration with emphasis on utilizing ion concentration polarization (ICP) effects in micro/nanofluidics platforms. We start with a brief introduction regarding the importance of preconcentration using micro/nanofluidics platforms, followed by in-depth discussions of the ICP effects for the preconcentration and applications to biomedical analysis, food testing and environmental monitoring. Finally, the article concludes with a brief perspective on the future development of the field.

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Section: Existing Sample Preconcentration Methodsmentioning

confidence: 99%

“…(d) shows that concentration factor of ∼1700 fold is achieved within around 10 min given use of 300 nm colloidal membrane. Redrawn from with permission from Royal Society of Chemistry.…”

Section: Existing Sample Preconcentration Methodsmentioning

confidence: 99%

Sample preconcentration from dilute solutions on micro/nanofluidic platforms: A review

Hou

Chiu

et al. 2017

Electrophoresis

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“…The application of nanofluidic crystal in ICP was further demonstrated in biomolecule concentration by Syed and co-workers (Figure 8(b)). 94 By using a 300 nm nanofluidic crystal as a nanojunction between two microfluidic channels, the authors were able to concentrate DNA molecules by 1700 times in 15 min and protein molecules by 100 times in 5 min. More recently, an active micromixer was developed with nanofluidic crystal, which took advantage of the strong convection generated by the non-equilibrium electrokinetics in the ion depletion zone (Figure 8(c)).…”

Section: Applicationsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Nanofluidic crystals: nanofluidics in a close-packed nanoparticle array

2017

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With various promising applications demonstrated, nanofluidics has been of broad research interest in the past decade. As nanofluidics matures from proof of concept towards practical applications, it faces two major barriers: expensive nanofabrication and ultra-low throughput. Up to date, the only material that enables nanofabrication-free, high-throughput, yet precisely controllable nanofluidic systems is the close-packed nanoparticle array, i.e. nanofluidic crystal. Recently, significant progresses in nanofluidics have been made using nanofluidic crystal, including high-current ionic diodes, high-power energy harvesters, efficient biomolecular separation, and facile biosensors. Nanofluidic crystal is seen as a key to applying nanofluidic concepts into real–world applications. In this review, we introduce the key concepts and models in nanofluidic crystal, summarize the fabrication methods, and discuss in-depth the various applications of nanofluidic crystal, highlighting its advantages in simple fabrication, low cost, flexibility, and high throughput. Finally, we provide our prospects on the future of nanofluidic crystal and its potential impacts.

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“…Self‐assembly is a simple way to fabricate NMI without the need for a clean room access, harmful chemicals, or applying high voltages. Self‐assembly of colloidal silica beads (300 nm in diameter) was reported for concentration of biomolecules by acting as a cation‐selective membrane . The device was fabricated in PDMS comprising five microchannels; a sample microchannel in the middle and a bead delivery and buffer microchannels on each side.…”

Section: Stackingmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016)

Breadmore

Wuethrich

et al. 2016

Electrophoresis

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One of the most cited limitations of capillary (and microchip) electrophoresis is the poor sensitivity. This review continues to update this series of biennial reviews, first published in Electrophoresis in 2007, on developments in the field of on-line/in-line concentration methods in capillaries and microchips, covering the period July 2014-June 2016. It includes developments in the field of stacking, covering all methods from field amplified sample stacking and large volume sample stacking, through to isotachophoresis, dynamic pH junction, and sweeping. Attention is also given to on-line or in-line extraction methods that have been used for electrophoresis.

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Creating sub-50 nm nanofluidic junctions in a PDMS microchip via self-assembly process of colloidal silica beads for electrokinetic concentration of biomolecules

Cited by 53 publications

References 27 publications

Sample preconcentration from dilute solutions on micro/nanofluidic platforms: A review

Sample preconcentration from dilute solutions on micro/nanofluidic platforms: A review

Nanofluidic crystals: nanofluidics in a close-packed nanoparticle array

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2014–2016)

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